JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M008494200 on October 6, 2000

J. Biol. Chem., Vol. 276, Issue 1, 729-737, January 5, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/1/729    most recent
M008494200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pedraza-Alva, G.
Right arrow Articles by Rosenstein, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pedraza-Alva, G.
Right arrow Articles by Rosenstein, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Regulation of Cbl Molecular Interactions by the Co-receptor Molecule CD43 in Human T Cells*

Gustavo Pedraza-AlvaDagger , Sansana Sawasdikosol§, Yun Cai Liu, Lily Beatriz MéridaDagger , Mario Ermesto Cruz-MuñozDagger , Fabian Oceguera-YañezDagger ||, Steven J. Burakoff§, and Yvonne RosensteinDagger **

From the Dagger  Instituto de Biotecnología/Universidad Nacional Autónoma de México, Apartado Postal 510-3, Cuernavaca, MOR 62250, Mexico, the § Dana Farber Cancer Institute, Boston, Massachusetts 02115, and the  La Jolla Institute for Allergy and Immunology, La Jolla, California 92121

CD43, one of the most abundant glycoproteins on the T cell surface, has been implicated in selection and maturation of thymocytes and migration, adhesion, and activation of mature T cells. The adapter molecule Cbl has been shown to be a negative regulator of Ras. Furthermore, it may also regulate intracellular signaling through the formation of several multi-molecular complexes. Here we investigated the role of Cbl in the CD43-mediated signaling pathway in human T cells. Unlike T cell receptor signaling, the interaction of the adapter protein Cbl with Vav and phosphatidylinositol 3-kinase, resulting from CD43-specific signals, is independent of Cbl tyrosine phosphorylation, suggesting an alternative mechanism of interaction. CD43 signals induced a Cbl serine phosphorylation-dependent interaction with the tau -isoform of 14-3-3. protein. Protein kinase C-mediated Cbl serine phosphorylation was required for this interaction, because the PKC inhibitor RO-31-8220 prevented it, as well as 14-3-3 dimerization. Moreover, mutation of Cbl serine residues 619, 623, 639, and 642 abolished the interaction between Cbl and 14-3-3. Overexpression of Cbl in Jurkat cells inhibited the CD43-dependent activation of the mitogen-activated protein kinase (MAPK) pathway and AP-1 transcriptional activity, confirming nevertheless a negative role for Cbl in T cell signaling. However, under normal conditions, PKC activation resulting from CD43 engagement was required to activate the MAPK pathway, suggesting that phosphorylation of Cbl on serine residues by PKC and its association with 14-3-3 molecules may play a role in preventing the Cbl inhibitory effect on the Ras-MAPK pathway. These data suggest that by inducing its phosphorylation on serine residues, CD43-mediated signals may regulate the molecular associations and functions of the Cbl adapter protein.

* This work was supported by Grant IN217498 from Dirección General de Apoyo al Personal Académico/Universidad Nacional Autónoma de México and Grant 25307-M from Consejo Nacional de Ciencia y Tecnologia, México.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| Present address: Kyoto University Faculty of Medicine. Sakyo-ku, Kyoto 606-8315, Japan.

** To whom correspondence should be addressed: Instituto de Biotecnología/UNAM, APDO Postal 510-3, Cuernavaca, MOR 62250, Mexico. Tel.: 52-73-291606; Fax: 52-73-172388; E-mail: yvonne@ibt.unam.mx.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
P. D. Mody, J. L. Cannon, H. S. Bandukwala, K. M. Blaine, A. B. Schilling, K. Swier, and A. I. Sperling
Signaling through CD43 regulates CD4 T-cell trafficking
Blood, October 15, 2007; 110(8): 2974 - 2982.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. M. Hatle, W. Neveu, O. Dienz, S. Rymarchyk, R. Barrantes, S. Hale, N. Farley, K. M. Lounsbury, J. P. Bond, D. Taatjes, et al.
Methylation-Controlled J Protein Promotes c-Jun Degradation To Prevent ABCB1 Transporter Expression
Mol. Cell. Biol., April 15, 2007; 27(8): 2952 - 2966.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Farley, G. Pedraza-Alva, D. Serrano-Gomez, V. Nagaleekar, A. Aronshtam, T. Krahl, T. Thornton, and M. Rincon
p38 Mitogen-Activated Protein Kinase Mediates the Fas-Induced Mitochondrial Death Pathway in CD8+ T Cells.
Mol. Cell. Biol., March 1, 2006; 26(6): 2118 - 2129.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. C. Fuhlbrigge, S. L. King, R. Sackstein, and T. S. Kupper
CD43 is a ligand for E-selectin on CLA+ human T cells
Blood, February 15, 2006; 107(4): 1421 - 1426.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Kyoizumi, T. Ohara, Y. Kusunoki, T. Hayashi, K. Koyama, and N. Tsuyama
Expression Characteristics and Stimulatory Functions of CD43 in Human CD4+ Memory T Cells: Analysis Using a Monoclonal Antibody to CD43 That Has a Novel Lineage Specificity
J. Immunol., June 15, 2004; 172(12): 7246 - 7253.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
E. Layseca-Espinosa, G. Pedraza-Alva, J. L. Montiel, R. del Rio, N. A. Fierro, R. Gonzalez-Amaro, and Y. Rosenstein
T cell aggregation induced through CD43: intracellular signals and inhibition by the immunomodulatory drug leflunomide
J. Leukoc. Biol., December 1, 2003; 74(6): 1083 - 1093.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
M. E. Cruz-Munoz, E. Salas-Vidal, N. Salaiza-Suazo, I. Becker, G. Pedraza-Alva, and Y. Rosenstein
The CD43 Coreceptor Molecule Recruits the {zeta}-Chain as Part of Its Signaling Pathway
J. Immunol., August 15, 2003; 171(4): 1901 - 1908.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. M. Onami, L. E. Harrington, M. A. Williams, M. Galvan, C. P. Larsen, T. C. Pearson, N. Manjunath, L. G. Baum, B. D. Pearce, and R. Ahmed
Dynamic Regulation of T Cell Immunity by CD43
J. Immunol., June 15, 2002; 168(12): 6022 - 6031.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Cermak, S. Simova, A. Pintzas, V. Horejsi, and L. Andera
Molecular Mechanisms Involved in CD43-mediated Apoptosis of TF-1 Cells. ROLES OF TRANSCRIPTION, Daxx EXPRESSION, AND ADHESION MOLECULES
J. Biol. Chem., March 1, 2002; 277(10): 7955 - 7961.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.