| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 10, 6897-6900, March 9, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
,, and§¶
From the Departments of Intestinal-enriched Krüppel-like
factor (IKLF or KLF5) belongs to the family of mammalian
Krüppel-like transcription factors. Previous studies indicate
that expression of IKLF is enriched in the proliferating
crypt epithelial cells of the intestinal tract. However, the biological
function of IKLF is unknown. In the current study, we have shown that
the level of IKLF mRNA was nearly undetectable in
serum-deprived NIH3T3 fibroblasts but became acutely and significantly
increased upon the addition of fetal bovine serum or the phorbol ester,
PMA. This induction required protein synthesis because it was prevented
by cycloheximide. Transfection of IKLF into NIH3T3 cells
resulted in the formation of foci in a manner similar to that caused by
the activated Ha-ras oncogene. Constitutive
expression of IKLF in transfected NIH3T3 cells
significantly increased the rate of proliferation when compared with
cells transfected with an empty vector. The growth of
IKLF-transfected cells was no longer inhibited by cell-cell
contact or by low serum content. Moreover, these cells proliferated in
an anchorage-independent fashion. We conclude that
IKLF encodes a delayed early response gene product that
positively regulates cellular proliferation and may give rise to a
transformed phenotype when overexpressed.
Medicine and
§ Biological Chemistry, The Johns Hopkins University School
of Medicine, Baltimore, Maryland 21205
This article has been cited by other articles:
|
|
 |
|
  B. G. Goldstein, H.-H. Chao, Y. Yang, Y. A. Yermolina, J. W. Tobias, and J. P. Katz Overexpression of Kruppel-like factor 5 in esophageal epithelia in vivo leads to increased proliferation in basal but not suprabasal cells Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1784 - G1792. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhang, A. Bialkowska, R. Rusovici, S. Chanchevalap, H. Shim, J. P. Katz, V. W. Yang, and C. C. Yun Lysophosphatidic Acid Facilitates Proliferation of Colon Cancer Cells via Induction of Kruppel-like Factor 5 J. Biol. Chem., May 25, 2007; 282(21): 15541 - 15549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. X. Du, C. C. Yun, A. Bialkowska, and V. W. Yang Protein Inhibitor of Activated STAT1 Interacts with and Up-regulates Activities of the Pro-proliferative Transcription Factor Kruppel-like Factor 5 J. Biol. Chem., February 16, 2007; 282(7): 4782 - 4793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Yang, B. G. Goldstein, H. Nakagawa, and J. P. Katz Kruppel-like factor 5 activates MEK/ERK signaling via EGFR in primary squamous epithelial cells FASEB J, February 1, 2007; 21(2): 543 - 550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Fumoto, T. Yamaguchi, F. Hirose, and T. Osumi Orphan Nuclear Receptor Nur77 Accelerates the Initial Phase of Adipocyte Differentiation in 3T3-L1 Cells by Promoting Mitotic Clonal Expansion J. Biochem., February 1, 2007; 141(2): 181 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Sur, B. Rozell, V. Jaks, A. Bergstrom, and R. Toftgard Epidermal and craniofacial defects in mice overexpressing Klf5 in the basal layer of the epidermis J. Cell Sci., September 1, 2006; 119(17): 3593 - 3601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Zhu, L. Gu, H. W. Findley, C. Chen, J.-T. Dong, L. Yang, and M. Zhou KLF5 Interacts with p53 in Regulating Survivin Expression in Acute Lymphoblastic Leukemia J. Biol. Chem., May 26, 2006; 281(21): 14711 - 14718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Tong, K. Czerwenka, G. Heinze, M. Ryffel, E. Schuster, A. Witt, S. Leodolter, and R. Zeillinger Expression of KLF5 is a Prognostic Factor for Disease-Free Survival and Overall Survival in Patients with Breast Cancer Clin. Cancer Res., April 15, 2006; 12(8): 2442 - 2448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chanchevalap, M. O. Nandan, B. B. McConnell, L. Charrier, D. Merlin, J. P. Katz, and V. W. Yang Kruppel-like factor 5 is an important mediator for lipopolysaccharide-induced proinflammatory response in intestinal epithelial cells Nucleic Acids Res., February 25, 2006; 34(4): 1216 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Suzuki, K. Aizawa, T. Matsumura, and R. Nagai Vascular Implications of the Kruppel-Like Family of Transcription Factors Arterioscler. Thromb. Vasc. Biol., June 1, 2005; 25(6): 1135 - 1141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. W. Bateman, D. Tan, R. G. Pestell, J. D. Black, and A. R. Black Intestinal Tumor Progression Is Associated with Altered Function of KLF5 J. Biol. Chem., March 26, 2004; 279(13): 12093 - 12101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Usui, N. Sugimoto, N. Takuwa, S. Sakagami, S. Takata, S. Kaneko, and Y. Takuwa Blood Lipid Mediator Sphingosine 1-Phosphate Potently Stimulates Platelet-derived Growth Factor-A and -B Chain Expression through S1P1-Gi-Ras-MAPK-dependent Induction of Kruppel-like Factor 5 J. Biol. Chem., March 26, 2004; 279(13): 12300 - 12311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Aizawa, T. Suzuki, N. Kada, A. Ishihara, K. Kawai-Kowase, T. Matsumura, K. Sasaki, Y. Munemasa, I. Manabe, M. Kurabayashi, et al. Regulation of Platelet-derived Growth Factor-A Chain by Kruppel-like Factor 5: NEW PATHWAY OF COOPERATIVE ACTIVATION WITH NUCLEAR FACTOR-{kappa}B J. Biol. Chem., January 2, 2004; 279(1): 70 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakamura, F. Unda, S. de-Vega, A. Vilaxa, S. Fukumoto, K. M. Yamada, and Y. Yamada The Kruppel-like Factor Epiprofin Is Expressed by Epithelium of Developing Teeth, Hair Follicles, and Limb Buds and Promotes Cell Proliferation J. Biol. Chem., January 2, 2004; 279(1): 626 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Miyamoto, T. Suzuki, S. Muto, K. Aizawa, A. Kimura, Y. Mizuno, T. Nagino, Y. Imai, N. Adachi, M. Horikoshi, et al. Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain Mol. Cell. Biol., December 1, 2003; 23(23): 8528 - 8541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang and C. T. Teng Phosphorylation of Kruppel-like factor 5 (KLF5/IKLF) at the CBP interaction region enhances its transactivation function Nucleic Acids Res., April 15, 2003; 31(8): 2196 - 2208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Chiambaretta, L. Blanchon, B. Rabier, W. W.-Y. Kao, J. J. Liu, B. Dastugue, D. Rigal, and V. Sapin Regulation of Corneal Keratin-12 Gene Expression by the Human Kruppel-like Transcription Factor 6 Invest. Ophthalmol. Vis. Sci., November 1, 2002; 43(11): 3422 - 3429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. T. Dang, W. Zhao, C. S. Mahatan, D. E. Geiman, and V. W. Yang Opposing effects of Kruppel-like factor 4 (gut-enriched Kruppel-like factor) and Kruppel-like factor 5 (intestinal-enriched Kruppel-like factor) on the promoter of the Kruppel-like factor 4 gene Nucleic Acids Res., July 1, 2002; 30(13): 2736 - 2741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Zhang, M. C. Moncrieffe, J. Kaczynski, V. Ellenrieder, F. G. Prendergast, and R. Urrutia A Conserved {alpha}-Helical Motif Mediates the Interaction of Sp1-Like Transcriptional Repressors with the Corepressor mSin3A Mol. Cell. Biol., August 1, 2001; 21(15): 5041 - 5049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Bieker Kruppel-like Factors: Three Fingers in Many Pies J. Biol. Chem., September 7, 2001; 276(37): 34355 - 34358. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
