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J. Biol. Chem., Vol. 276, Issue 10, 6950-6958, March 9, 2001
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,,
From the Expression of the structural genes for alcohol
and aldehyde dehydrogenase, alcA and aldA,
respectively, enables the fungus Aspergillus nidulans to
grow on ethanol. The pathway-specific transcriptional activator AlcR
mediates the induction of ethanol catabolism in the presence of a
coinducing compound. Ethanol catabolism is further subject to negative
control mediated by the general carbon catabolite repressor CreA. Here
we show that, in contrast to alcA and alcR, the
aldA gene is not directly subject to CreA repression. A
single cis-acting element mediates AlcR activation of
aldA. Furthermore, we show that the induction of the
alc gene system is linked to in situ aldehyde
dehydrogenase activity. In aldA loss-of-function mutants,
the alc genes are induced under normally noninducing
conditions. This pseudo-constitutive expression correlates with the
nature of the mutations, suggesting that this feature is caused by an
intracellular accumulation of a coinducing compound. Conversely,
constitutive overexpression of aldA results in suppression
of induction in the presence of ethanol. This shows unambiguously that
acetaldehyde is the sole physiological inducer of ethanol catabolism.
We hypothesize that the intracellular acetaldehyde concentration is the
critical factor governing the induction of the alc gene system.
Institut de Génétique et
Microbiologie, CNRS UMR 8621, Université Paris-Sud XI, Centre
Universitaire d'Orsay, Bâtiment 409, F-91405 Orsay Cedex, France
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF260123, AF260124, AF260125, and AF260126.
§ Supported by the Erasmus student exchange program. Present address: Fachbereich Biologie, Philipps-Universität Marburg, Karl-von Frischstrasse, D-35032 Marburg, Germany. ¶ Present address: Laboratoire de Neurogénétique Moléculaire, E. 9913, GENOPOLE, 2 Rue Gaston Crémieux, CP5724, F-91057 Evry Cedex, France.
To whom correspondence should be addressed. Tel.: 33 1 69156328; Fax: 33 1 69157808; E-mail: felenbok@igmors.u-psud.fr.
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