HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 11, 7943-7951, March 16, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/11/7943    most recent M009836200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Diamond, P. Articles by Coles, L. S. Search for Related Content PubMed PubMed Citation Articles by Diamond, P. Articles by Coles, L. S.

Cold Shock Domain Factors Activate the Granulocyte-Macrophage Colony-stimulating Factor Promoter in Stimulated Jurkat T Cells^*

Peter Diamond^§, M. Frances Shannon^¶, Mathew A. Vadas, and Leeanne S. Coles

From the  Division of Human Immunology, Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Frome Road, Adelaide, South Australia, 5000 and the ^¶ Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra, Australian Capital Territory 2601, Australia

Cold shock domain (CSD) family members have been shown to play roles in either transcriptional activation or repression of many genes in various cell types. We have previously shown that CSD proteins dbpAv and dbpB (also known as YB-1) act to repress granulocyte-macrophage colony-stimulating factor transcription in human embryonic lung (HEL) fibroblasts via binding to single-stranded DNA regions across the promoter. Here we show that the same CSD factors are involved in granulocyte-macrophage colony-stimulating factor transcriptional activation in Jurkat T cells. Unlike the mechanisms of CSD repression in HEL fibroblasts, CSD-mediated activation in Jurkat T cells is not mediated through DNA binding but presumably through protein-protein interactions via the C terminus of the CSD protein with transcription factors such as RelA/NF-B p65. We demonstrate that Jurkat T cells lack truncated CSD factor subtypes present in HEL fibroblasts, which raises the possibility that the cellular content of CSD proteins may determine their final role as activators or repressors of transcription.

This article has been cited by other articles:

				C. R.C. van Roeyen, F. Eitner, S. Martinkus, S. R. Thieltges, T. Ostendorf, D. Bokemeyer, B. Luscher, J. M. Luscher-Firzlaff, J. Floege, and P. R. Mertens Y-Box Protein 1 Mediates PDGF-B Effects in Mesangioproliferative Glomerular Disease J. Am. Soc. Nephrol., October 1, 2005; 16(10): 2985 - 2996. [Abstract] [Full Text] [PDF]

				L. S. Coles, P. Diamond, L. Lambrusco, J. Hunter, J. Burrows, M. A. Vadas, and G. J. Goodall A novel mechanism of repression of the vascular endothelial growth factor promoter, by single strand DNA binding cold shock domain (Y-box) proteins in normoxic fibroblasts Nucleic Acids Res., November 15, 2002; 30(22): 4845 - 4854. [Abstract] [Full Text] [PDF]

				J. T. Norman, G. E. Lindahl, K. Shakib, A. En-Nia, E. Yilmaz, and P. R. Mertens The Y-box Binding Protein YB-1 Suppresses Collagen alpha 1(I) Gene Transcription via an Evolutionarily Conserved Regulatory Element in the Proximal Promoter J. Biol. Chem., August 3, 2001; 276(32): 29880 - 29890. [Abstract] [Full Text] [PDF]