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J. Biol. Chem., Vol. 276, Issue 12, 8643-8647, March 23, 2001
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From the Institute of Organic Chemistry, University of Fribourg,
Chemin du Musée 9, CH-1700 Fribourg, Switzerland
A new type of chlorophyll catabolite was isolated
from extracts of de-greened primary leaves of barley (Hordeum
vulgare cv. Lambic). Its constitution was elucidated by
one-dimensional and two-dimensional
{1H,13C}-homo- and heteronuclear NMR
spectroscopic techniques and by high resolution mass spectroscopy. The
isolated catabolite, a water-soluble, colorless, and nonfluorescent
linear tetrapyrrole, resembles urobilinogen in which one of the
propionic side chains forms a five membered isocylic ring system,
indicating its origin from the chlorophylls.
Isolation and Characterization of a Urobilinogenoidic Chlorophyll
Catabolite from Hordeum vulgare L.*
*
This work was supported by the Swiss National Science
Foundation (Project No. 2000-50725.97/1).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.: 41-26-300-8785;
Fax: 41-26-300-9739; E-mail: norbert.engel@unifr.ch.
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