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Originally published In Press as doi:10.1074/jbc.M009288200 on December 13, 2000

J. Biol. Chem., Vol. 276, Issue 12, 8643-8647, March 23, 2001
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Isolation and Characterization of a Urobilinogenoidic Chlorophyll Catabolite from Hordeum vulgare L.*

Fosca Gattoni Losey and Norbert EngelDagger

From the Institute of Organic Chemistry, University of Fribourg, Chemin du Musée 9, CH-1700 Fribourg, Switzerland

A new type of chlorophyll catabolite was isolated from extracts of de-greened primary leaves of barley (Hordeum vulgare cv. Lambic). Its constitution was elucidated by one-dimensional and two-dimensional {1H,13C}-homo- and heteronuclear NMR spectroscopic techniques and by high resolution mass spectroscopy. The isolated catabolite, a water-soluble, colorless, and nonfluorescent linear tetrapyrrole, resembles urobilinogen in which one of the propionic side chains forms a five membered isocylic ring system, indicating its origin from the chlorophylls.


* This work was supported by the Swiss National Science Foundation (Project No. 2000-50725.97/1).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed. Tel.: 41-26-300-8785; Fax: 41-26-300-9739; E-mail: norbert.engel@unifr.ch.


Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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