HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 12, 8904-8909, March 23, 2001

This Article Full Text Full Text (PDF) An addition or correction has been published All Versions of this Article: 276/12/8904    most recent M009527200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jankowski, J. Articles by Schlüter, H. Search for Related Content PubMed PubMed Citation Articles by Jankowski, J. Articles by Schlüter, H. Social Bookmarking                      What's this?

Dinucleotides as Growth-promoting Extracellular Mediators
PRESENCE OF DINUCLEOSIDE DIPHOSPHATES Ap₂A, Ap₂G, AND Gp₂G IN RELEASABLE GRANULES OF PLATELETS^*

Joachim Jankowski, Joost Hagemann, Martin Tepel, Markus van der Giet, Nina Stephan, Lars Henning, Helena Gouni-Berthold^§, Agapios Sachinidis^¶, Walter Zidek, and Hartmut Schlüter

From the  Medizinische Klinik IV, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Berlin 12200, the ^§ Medizinische Universitätspoliklinik, Bonn D-53111, and the ^¶ Institut für Neurophysiologie, Universität zu Köln, Köln D-50931, Germany

Dinucleoside diphosphates, Ap₂A, Ap₂G, and Gp₂G represent a new class of growth-promoting extracellular mediators, which are released from granules after activation of platelets. The presence of theses substances was shown after purification from a platelet concentrate. The substances were identified by UV spectrometry, retention time comparison with authentic substances, matrix-assisted laser desorption/ionization mass spectrometry, post-source-decay matrix-assisted laser desorption/ionization mass spectrometry, and enzymatic analysis. Ap₂A, Ap₂G, and Gp₂G have growth-stimulating effects on vascular smooth muscle cells in nanomolar concentrations as shown by [³H]thymidine incorporation measurements. The calculated EC₅₀ (log M; mean ± S.E.) values were 6.07 ± 0.14 for Ap₂A, 6.27 ± 0.25 for Ap₂G, and 6.91 ± 0.44 for Gp₂G. At least 61.5 ± 4.3% of the dinucleoside polyphosphates are released by platelet activation. The intraplatelet concentrations suggest that, in the close environment of a platelet thrombus, similar dinucleoside polyphosphate concentrations can be found as in platelets. Intraplatelet concentration can be estimated in the range of 1/20 to 1/100 of the concentration of ATP. In conclusion, Ap₂A, Ap₂G, and Gp₂G derived from releasable granules of human platelets may play a regulatory role in vascular smooth muscle growth as growth-promoting mediators.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. Magnone, G. Basile, D. Bruzzese, L. Guida, M. G. Signorello, M. P. Chothi, S. Bruzzone, E. Millo, A.-D. Qi, R. A. Nicholas, et al. Adenylic Dinucleotides Produced by CD38 Are Negative Endogenous Modulators of Platelet Aggregation J. Biol. Chem., September 5, 2008; 283(36): 24460 - 24468. [Abstract] [Full Text] [PDF]

				S. Bruzzone, G. Dodoni, N. Kaludercic, G. Basile, E. Millo, A. De Flora, F. Di Lisa, and E. Zocchi Mitochondrial Dysfunction Induced by a Cytotoxic Adenine Dinucleotide Produced by ADP-ribosyl Cyclases from cADPR J. Biol. Chem., February 16, 2007; 282(7): 5045 - 5052. [Abstract] [Full Text] [PDF]

				T. F. Walseth A multifunctional enzyme adds to its repertoire PNAS, October 18, 2005; 102(42): 14941 - 14942. [Full Text] [PDF]

				J. Luo, V. Jankowski, N. Gungar, J. Neumann, W. Schmitz, W. Zidek, H. Schluter, and J. Jankowski Endogenous Diadenosine Tetraphosphate, Diadenosine Pentaphosphate, and Diadenosine Hexaphosphate in Human Myocardial Tissue Hypertension, May 1, 2004; 43(5): 1055 - 1059. [Abstract] [Full Text] [PDF]

				J. Jankowski, V. Jankowski, U. Laufer, M. van der Giet, L. Henning, M. Tepel, W. Zidek, and H. Schluter Identification and Quantification of Diadenosine Polyphosphate Concentrations in Human Plasma Arterioscler. Thromb. Vasc. Biol., July 1, 2003; 23(7): 1231 - 1238. [Abstract] [Full Text] [PDF]

				G. Gabriels, K. H. Rahn, E. Schlatter, and M. Steinmetz Mesenteric and renal vascular effects of diadenosine polyphosphates (APnA) Cardiovasc Res, October 1, 2002; 56(1): 22 - 32. [Abstract] [Full Text] [PDF]