Advertisement
JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M009963200 on December 15, 2000

J. Biol. Chem., Vol. 276, Issue 12, 9166-9173, March 23, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/12/9166    most recent
M009963200v1
Right arrow Submit a Letter to Editor
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Karihaloo, A.
Right arrow Articles by Cantley, L. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Karihaloo, A.
Right arrow Articles by Cantley, L. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Differential MAPK Pathways Utilized for HGF- and EGF-dependent Renal Epithelial Morphogenesis*

Anil KarihalooDagger §, Dawn A. O'Rourke§||, Christian NickelDagger , Katherine Spokes**, and Lloyd G. CantleyDagger

From the Dagger  School of Medicine, Yale University, New Haven, Connecticut 06520, || ACLARA Biosciences, Mountain View, California 94043, and ** Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215

Cells derived from the inner medullary collecting duct undergo in vitro branching tubulogenesis to both the c-met receptor ligand hepatocyte growth factor (HGF) as well as epidermal growth factor (EGF) receptor ligands. In contrast, many other cultured renal epithelial cells respond in this manner only to HGF, suggesting that these two receptors may use independent signaling pathways during morphogenesis. We have therefore compared the signaling pathways for mIMCD-3 cell morphogenesis in response to EGF and HGF. Inhibition of the p42/44 mitogen-activated protein kinase (MAPK) pathway with the mitogen-activated protein kinase kinase (MKK1) inhibitor PD98059 (50 µM) markedly inhibits HGF-induced cell migration with only partial inhibition of EGF-induced cell motility. Similarly, HGF-dependent, but not EGF-dependent, branching morphogenesis was more greatly inhibited by the MKK1 inhibitor. Examination of EGF-stimulated cells demonstrated that extracellular-regulated kinase 5 (ERK5) was activated in response to EGF but not HGF, and that activation of ERK5 was only 60% inhibited by 50 µM PD98059. In contrast, the MKK inhibitor U0126 markedly inhibited both ERK1/2 and ERK5 activation and completely prevented HGF- and EGF-dependent migration and branching process formation. Expression of dominant negative ERK5 (dnBMK1) likewise inhibited EGF-dependent branching process formation, but did not affect HGF-dependent branching process formation. Our results indicate that activation of the ERK1/ERK2 signaling pathway is critical for HGF-induced cell motility/morphogenesis in mIMCD-3 cells, whereas ERK5 appears to be required for EGF-dependent morphogenesis.

* This work was supported by National Institutes of Health Grant DK54911 (to L. G. C.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Both authors contributed equally to this work.

To whom correspondence should be addressed: Yale University School of Medicine, 333 Cedar St., LMP 2093, New Haven, CT 06520. Tel.: 203-785-7111; Fax: 203-785-7068; E-mail: anil.karihaloo@yale.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
S. Ishibe, A. Karihaloo, H. Ma, J. Zhang, A. Marlier, M. Mitobe, A. Togawa, R. Schmitt, J. Czyczk, M. Kashgarian, et al.
Met and the epidermal growth factor receptor act cooperatively to regulate final nephron number and maintain collecting duct morphology
Development, January 15, 2009; 136(2): 337 - 345.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
V. Arnoux, M. Nassour, A. L'Helgoualc'h, R. A. Hipskind, and P. Savagner
Erk5 Controls Slug Expression and Keratinocyte Activation during Wound Healing
Mol. Biol. Cell, November 1, 2008; 19(11): 4738 - 4749.
[Abstract] [Full Text] [PDF]

Home page
 LupusHome page
G Ferraccioli and G Romano
Renal interstitial cells, proteinuria and progression of lupus nephritis: new frontiers for old factors
Lupus, June 1, 2008; 17(6): 533 - 540.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
L. C. Gahring, A. V. Osborne-Hereford, G. A. Vasquez-Opazo, and S. W. Rogers
Tumor Necrosis Factor {alpha} Enhances Nicotinic Receptor Up-regulation via a p38MAPK-dependent Pathway
J. Biol. Chem., January 11, 2008; 283(2): 693 - 699.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. Tsuda, I. J. Davis, P. Argani, N. Shukla, G. G. McGill, M. Nagai, T. Saito, M. Lae, D. E. Fisher, and M. Ladanyi
TFE3 Fusions Activate MET Signaling by Transcriptional Up-regulation, Defining Another Class of Tumors as Candidates for Therapeutic MET Inhibition
Cancer Res., February 1, 2007; 67(3): 919 - 929.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Ishibe, J. E. Haydu, A. Togawa, A. Marlier, and L. G. Cantley
Cell Confluence Regulates Hepatocyte Growth Factor-Stimulated Cell Morphogenesis in a {beta}-Catenin-Dependent Manner
Mol. Cell. Biol., December 15, 2006; 26(24): 9232 - 9243.
[Abstract] [Full Text] [PDF]

Home page
 Drug Metab. Dispos.Home page
S. K. Kim, M. A. Abdelmegeed, and R. F. Novak
THE MITOGEN-ACTIVATED PROTEIN KINASE KINASE (MEK) INHIBITOR PD98059 ELEVATES PRIMARY CULTURED RAT HEPATOCYTE GLUTATHIONE LEVELS INDEPENDENT OF INHIBITING MEK
Drug Metab. Dispos., April 1, 2006; 34(4): 683 - 689.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
I. V. Yosypiv, M. Schroeder, and S. S. El-Dahr
Angiotensin II Type 1 Receptor-EGF Receptor Cross-Talk Regulates Ureteric Bud Branching Morphogenesis
J. Am. Soc. Nephrol., April 1, 2006; 17(4): 1005 - 1014.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. H. Grimm, A. Karihaloo, Y. Cai, S. Somlo, L. G. Cantley, and M. J. Caplan
Polycystin-2 Regulates Proliferation and Branching Morphogenesis in Kidney Epithelial Cells
J. Biol. Chem., January 6, 2006; 281(1): 137 - 144.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
R. R. Mattingly, J. M. Kraniak, J. T. Dilworth, P. Mathieu, B. Bealmear, J. E. Nowak, J. A. Benjamins, M. A. Tainsky, and J. J. Reiners Jr.
The Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Kinase Inhibitor PD184352 (CI-1040) Selectively Induces Apoptosis in Malignant Schwannoma Cell Lines
J. Pharmacol. Exp. Ther., January 1, 2006; 316(1): 456 - 465.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Karihaloo, S. A. Karumanchi, W. L. Cantley, S. Venkatesha, L. G. Cantley, and S. Kale
Vascular Endothelial Growth Factor Induces Branching Morphogenesis/Tubulogenesis in Renal Epithelial Cells in a Neuropilin-Dependent Fashion
Mol. Cell. Biol., September 1, 2005; 25(17): 7441 - 7448.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Y. Nishikawa, Y. Doi, H. Watanabe, T. Tokairin, Y. Omori, M. Su, T. Yoshioka, and K. Enomoto
Transdifferentiation of Mature Rat Hepatocytes into Bile Duct-Like Cells in Vitro
Am. J. Pathol., April 1, 2005; 166(4): 1077 - 1088.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. A. Bromann, H. Korkaya, C. P. Webb, J. Miller, T. L. Calvin, and S. A. Courtneidge
Platelet-derived Growth Factor Stimulates Src-dependent mRNA Stabilization of Specific Early Genes in Fibroblasts
J. Biol. Chem., March 18, 2005; 280(11): 10253 - 10263.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. A. Gwira, F. Wei, S. Ishibe, J. M. Ueland, J. Barasch, and L. G. Cantley
Expression of Neutrophil Gelatinase-associated Lipocalin Regulates Epithelial Morphogenesis in Vitro
J. Biol. Chem., March 4, 2005; 280(9): 7875 - 7882.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Marchetti, C. Gimond, J.-C. Chambard, T. Touboul, D. Roux, J. Pouyssegur, and G. Pages
Extracellular Signal-Regulated Kinases Phosphorylate Mitogen-Activated Protein Kinase Phosphatase 3/DUSP6 at Serines 159 and 197, Two Sites Critical for Its Proteasomal Degradation
Mol. Cell. Biol., January 15, 2005; 25(2): 854 - 864.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Karihaloo, S. Kale, N. D. Rosenblum, and L. G. Cantley
Hepatocyte Growth Factor-Mediated Renal Epithelial Branching Morphogenesis Is Regulated by Glypican-4 Expression
Mol. Cell. Biol., October 1, 2004; 24(19): 8745 - 8752.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Li, E. R. Gerrard Jr., and D. F. Balkovetz
Evidence for ERK1/2 phosphorylation controlling contact inhibition of proliferation in Madin-Darby canine kidney epithelial cells
Am J Physiol Cell Physiol, August 1, 2004; 287(2): C432 - C439.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. J. Han, W. J. Sigurdson, P. A. Nickerson, and M. Taub
Both mitogen activated protein kinase and the mammalian target of rapamycin modulate the development of functional renal proximal tubules in matrigel
J. Cell Sci., May 1, 2004; 117(9): 1821 - 1833.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Klingenberg, D. Gunduz, F. Hartel, K. Bindewald, M. Schafer, H. M. Piper, and T. Noll
MEK/MAPK as a signaling element in ATP control of endothelial myosin light chain
Am J Physiol Cell Physiol, April 1, 2004; 286(4): C807 - C812.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. C. Hu, D. Wasserman, S. Hartwig, and N. D. Rosenblum
p38MAPK Acts in the BMP7-dependent Stimulatory Pathway during Epithelial Cell Morphogenesis and Is Regulated by Smad1
J. Biol. Chem., March 26, 2004; 279(13): 12051 - 12059.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. M. Ueland, J. Gwira, Z.-X. Liu, and L. G. Cantley
The chemokine KC regulates HGF-stimulated epithelial cell morphogenesis
Am J Physiol Renal Physiol, March 1, 2004; 286(3): F581 - F589.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
B.-e Xu, S. Stippec, L. Lenertz, B.-H. Lee, W. Zhang, Y.-K. Lee, and M. H. Cobb
WNK1 Activates ERK5 by an MEKK2/3-dependent Mechanism
J. Biol. Chem., February 27, 2004; 279(9): 7826 - 7831.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. K. Brar, A. K. Jonassen, E. M. Egorina, A. Chen, A. Negro, M. H. Perrin, O. D. Mjos, D. S. Latchman, K.-F. Lee, and W. Vale
Urocortin-II and Urocortin-III Are Cardioprotective against Ischemia Reperfusion Injury: An Essential Endogenous Cardioprotective Role for Corticotropin Releasing Factor Receptor Type 2 in the Murine Heart
Endocrinology, January 1, 2004; 145(1): 24 - 35.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. G. Christensen, R. Schreck, J. Burrows, P. Kuruganti, E. Chan, P. Le, J. Chen, X. Wang, L. Ruslim, R. Blake, et al.
A Selective Small Molecule Inhibitor of c-Met Kinase Inhibits c-Met-Dependent Phenotypes in Vitro and Exhibits Cytoreductive Antitumor Activity in Vivo
Cancer Res., November 1, 2003; 63(21): 7345 - 7355.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
H. Zhao, W. Tian, C. Tai, and D. M. Cohen
Hypertonic induction of COX-2 expression in renal medullary epithelial cells requires transactivation of the EGFR
Am J Physiol Renal Physiol, August 1, 2003; 285(2): F281 - F288.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Hunger-Glaser, E. P. Salazar, J. Sinnett-Smith, and E. Rozengurt
Bombesin, Lysophosphatidic Acid, and Epidermal Growth Factor Rapidly Stimulate Focal Adhesion Kinase Phosphorylation at Ser-910: REQUIREMENT FOR ERK ACTIVATION
J. Biol. Chem., June 13, 2003; 278(25): 22631 - 22643.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. J. Cameron, S. Malik, M. Akaike, N. Lerner-Marmarosh, C. Yan, J.-D. Lee, J.-i. Abe, and J. Yang
Regulation of Epidermal Growth Factor-induced Connexin 43 Gap Junction Communication by Big Mitogen-activated Protein Kinase 1/ERK5 but Not ERK1/2 Kinase Activation
J. Biol. Chem., May 9, 2003; 278(20): 18682 - 18688.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. E. Hammond, S. Carter, J. McCullough, S. Urbe, G. Vande Woude, and M. J. Clague
Endosomal Dynamics of Met Determine Signaling Output
Mol. Biol. Cell, April 1, 2003; 14(4): 1346 - 1354.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. F. Yu, Z.-X. Liu, and L. G. Cantley
ERK Negatively Regulates the Epidermal Growth Factor-mediated Interaction of Gab1 and the Phosphatidylinositol 3-Kinase
J. Biol. Chem., May 24, 2002; 277(22): 19382 - 19388.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z.-X. Liu, C. F. Yu, C. Nickel, S. Thomas, and L. G. Cantley
Hepatocyte Growth Factor Induces ERK-dependent Paxillin Phosphorylation and Regulates Paxillin-Focal Adhesion Kinase Association
J. Biol. Chem., March 15, 2002; 277(12): 10452 - 10458.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
D. E. Kling, H. K. Lorenzo, A. M. Trbovich, T. B. Kinane, P. K. Donahoe, and J. J. Schnitzer
Pre- and Postnatal Lung Development, Maturation, and Plasticity: MEK-1/2 inhibition reduces branching morphogenesis and causes mesenchymal cell apoptosis in fetal rat lungs
Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L370 - L378.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Karihaloo, S. A. Karumanchi, J. Barasch, V. Jha, C. H. Nickel, J. Yang, S. Grisaru, K. T. Bush, S. Nigam, N. D. Rosenblum, et al.
Endostatin regulates branching morphogenesis of renal epithelial cells and ureteric bud
PNAS, October 12, 2001; (2001) 221205198.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. F. Yu, B. Roshan, Z.-X. Liu, and L. G. Cantley
ERK Regulates the Hepatocyte Growth Factor-mediated Interaction of Gab1 and the Phosphatidylinositol 3-Kinase
J. Biol. Chem., August 24, 2001; 276(35): 32552 - 32558.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Karihaloo, S. A. Karumanchi, J. Barasch, V. Jha, C. H. Nickel, J. Yang, S. Grisaru, K. T. Bush, S. Nigam, N. D. Rosenblum, et al.
Endostatin regulates branching morphogenesis of renal epithelial cells and ureteric bud
PNAS, October 23, 2001; 98(22): 12509 - 12514.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement