| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 12, 9413-9420, March 23, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
and
From the Laboratory of Molecular Genetics, NIEHS, National
Institutes of Health, Research Triangle Park,
North Carolina 27709
This study examines the interaction between
Escherichia coli MutS,L and E. coli RuvAB
during E. coli RecA-promoted strand exchange. RuvAB is a
branch migration complex that stimulates heterologous strand exchange.
Previous studies indicate that RuvAB increases the rate at which
heteroduplex products are formed by RecA, that RuvA and RuvB are
required for this stimulation, and that RuvAB does not stimulate
homologous strand exchange. This study indicates that MutS,L inhibit
the formation of full-length heteroduplex DNA between M13-fd DNA
in the presence of RuvAB, such that less than 2% of the linear
substrate is converted to product. Inhibition depends on the time at
which MutS,L are added to the reaction and is strongest when MutS,L are
added during initiation. The kinetics of the strand exchange reaction
suggest that MutS,L directly inhibit RuvAB-dependent branch
migration in the absence of RecA. The inhibition requires the formation of base-base mismatches and ATP utilization; no effect on
RuvAB-promoted strand exchange is seen if an ATP-deficient mutant of
MutS (MutS501) is included in the reaction instead of wild-type MutS.
These results are consistent with a role for MutS,L in maintaining
genomic stability and replication fidelity.
Permanent address: Inst. of Biochemistry and Biophysics,
Polish Academy of Science, Pawinskiego 5a, 02-106 Warsaw, Poland.
§
To whom correspondence and reprint requests should be addressed.
Tel.: 919-541-0670; Fax: 919-541-7593; E-mail:
worth@niehs.nih.gov.
This article has been cited by other articles:
|
|
 |
|
  M. Elez, M. Radman, and I. Matic The frequency and structure of recombinant products is determined by the cellular level of MutL PNAS, May 22, 2007; 104(21): 8935 - 8940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Yang, E. B. Goldsmith, Y. Lin, B. C. Waldman, V. Kaza, and A. S. Waldman Genetic Exchange Between Homeologous Sequences in Mammalian Chromosomes Is Averted by Local Homology Requirements for Initiation and Resolution of Recombination Genetics, September 1, 2006; 174(1): 135 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Calmann, A. Nowosielska, and M. G. Marinus Separation of mutation avoidance and antirecombination functions in an Escherichia coli mutS mutant Nucleic Acids Res., February 24, 2005; 33(4): 1193 - 1200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Goldfarb and E. Alani Distinct Roles for the Saccharomyces cerevisiae Mismatch Repair Proteins in Heteroduplex Rejection, Mismatch Repair and Nonhomologous Tail Removal Genetics, February 1, 2005; 169(2): 563 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Meier and W. Wackernagel Impact of mutS Inactivation on Foreign DNA Acquisition by Natural Transformation in Pseudomonas stutzeri J. Bacteriol., January 1, 2005; 187(1): 143 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Calmann and M. G. Marinus MutS inhibits RecA-mediated strand exchange with platinated DNA substrates PNAS, September 28, 2004; 101(39): 14174 - 14179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Jelesko, K. Carter, W. Thompson, Y. Kinoshita, and W. Gruissem Meiotic Recombination Between Paralogous RBCSB Genes on Sister Chromatids of Arabidopsis thaliana Genetics, February 1, 2004; 166(2): 947 - 957. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Costantino and D. L. Court Enhanced levels of {lambda} Red-mediated recombinants in mismatch repair mutants PNAS, December 23, 2003; 100(26): 15748 - 15753. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
