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Originally published In Press as doi:10.1074/jbc.M008696200 on December 4, 2000

J. Biol. Chem., Vol. 276, Issue 13, 9662-9669, March 30, 2001
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Cell Cycle Regulation of the Murine cdc25B Promoter
ESSENTIAL ROLE FOR NUCLEAR FACTOR-Y AND A PROXIMAL REPRESSOR ELEMENT*

Kathrin Körner, Valérie Jérôme, Thorsten Schmidt, and Rolf MüllerDagger

From the Institute of Molecular Biology and Tumor Research (IMT), Philipps University, Emil-Mannkopff-Strasse 2, 35033 Marburg, Germany

Expression of the cdc25B gene is up-regulated late during cell cycle progression (S/G2). We have cloned the murine cdc25B promoter to identify elements involved in transcriptional regulation. A detailed structure-function analysis led to the identification of several elements that are located upstream of a canonical Inr motif at the site of transcription initiation and are involved in transcriptional activation and regulation. Activation of the promoter is largely mediated by NF-Y and Sp1/3 interacting with one and four proximal binding sites, respectively. In addition, NF-Y plays an essential role in cell cycle regulation in conjunction with a repressor element (cell cycle-regulated repressor) located ~30 nucleotides upstream of the putative Inr element and overlapping a consensus TATA motif. The cell cycle-regulated repressor is unrelated to the previously described cell cycle-regulated repressor elements. Taken together, our observations suggest that expression of the cdc25B gene is controlled through a novel mechanism of cell cycle-regulated transcription.

* This work was supported by grants from the Deutsche Forschungsgemeinschaft (SFB397/C1, Mu601/9-2).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ296019.

Dagger To whom correspondence and requests for reprints should be addressed: Institut für Molekularbiologie und Tumorforschung (IMT), Emil-Mannkopff-Strasse 2, 35033 Marburg, Germany. Tel.: 49 6421 28 66236; Fax: 49 6421 28 68923; E-mail: mueller@imt.uni-marburg.de.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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