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Originally published In Press as doi:10.1074/jbc.M010611200 on January 8, 2001

J. Biol. Chem., Vol. 276, Issue 14, 10745-10752, April 6, 2001
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A Novel Member of the Bacterial-Archaeal Regulator Family Is a Nonspecific DNA-binding Protein and Induces Positive Supercoiling*

Alessandra NapoliDagger , Mamuka Kvaratskelia§, Malcolm F. White§, Mosé RossiDagger , and Maria CiaramellaDagger

From the Dagger  Institute of Protein Biochemistry and Enzymology, Consiglio Nazionale delle Ricerche, Via Marconi 10, 80125 Naples, Italy and the § Centre for Biomolecular Science, St. Andrews University, KY16 9ST, St. Andrews, United Kingdom

In hyperthermophilic Archaea genomic DNA is from relaxed to positively supercoiled in vivo because of the action of the enzyme reverse gyrase, and this peculiarity is believed to be related to stabilization of DNA against denaturation. We report the identification and characterization of Smj12, a novel protein of Sulfolobus solfataricus, which is homologous to members of the so-called Bacterial-Archaeal family of regulators, found in multiple copies in Eubacteria and Archaea. Whereas other members of the family are sequence-specific DNA- binding proteins and have been implicated in transcriptional regulation, Smj12 is a nonspecific DNA-binding protein that stabilizes the double helix and induces positive supercoiling. Smj12 is not abundant, suggesting that it is not a general architectural protein, but rather has a specialized function and/or localization. Smj12 is the first protein with the described features identified in Archaea and might participate in control of superhelicity during DNA transactions.

* This work was partially supported by the European Union project "Extremophiles as cell factories" and by the CNR Special Program "Biomolecole per la salute umana."The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ133494.

To whom correspondence should be addressed. Tel.: 390817257246; Fax: 390812396525; E-mail: ciaramel@dafne.ibpe.na.cnr.it.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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