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Originally published In Press as doi:10.1074/jbc.M010244200 on November 27, 2000

J. Biol. Chem., Vol. 276, Issue 14, 11302-11309, April 6, 2001
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Subunit II of Cytochrome c Oxidase in Chlamydomonad Algae Is a Heterodimer Encoded by Two Independent Nuclear Genes*

Xochitl Pérez-MartínezDagger , Anaid AntaramianDagger , Miriam Vázquez-AcevedoDagger , Soledad FunesDagger , Elena Tolkunova§, Jacques d'Alayer, Manuel G. Claros||, Edgar Davidson§, Michael P. King§, and Diego González-HalphenDagger **

From the Dagger  Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Apartado Postal 70-243, México 04510, D.F. Mexico, § Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107,  Laboratoire de Microséquençage des Protéines, Département des Biotechnologies, Institut Pasteur, 75724 Paris Cedex 15, France, and || Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga Spain

The mitochondrial genomes of Chlamydomonad algae lack the cox2 gene that encodes the essential subunit COX II of cytochrome c oxidase. COX II is normally a single polypeptide encoded by a single mitochondrial gene. In this work we cloned two nuclear genes encoding COX II from both Chlamydomonas reinhardtii and Polytomella sp. The cox2a gene encodes a protein, COX IIA, corresponding to the N-terminal portion of subunit II of cytochrome c oxidase, and the cox2b gene encodes COX IIB, corresponding to the C-terminal region. The cox2a and cox2b genes are located in the nucleus and are independently transcribed into mRNAs that are translated into separate polypeptides. These two proteins assemble with other cytochrome c oxidase subunits in the inner mitochondrial membrane to form the mature multi-subunit complex. We propose that during the evolution of the Chlorophyte algae, the cox2 gene was divided into two mitochondrial genes that were subsequently transferred to the nucleus. This event was evolutionarily distinct from the transfer of an intact cox2 gene to the nucleus in some members the Leguminosae plant family.

* This work was supported by National Institutes of Health Grants TW01176 and HL59646, Consejo Nacional de Ciencia y Tecnologia, Mexico Grant 27754N, and Dirección General de Apoyo al Personal Académico, Universidad Nacional Autónoma de México Grant IN202598.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF305078 (Polytomella sp. cox2a cDNA), AF305079 (Polytomella sp. cox2b cDNA), AF305080 (C. reinhardtii cox2a cDNA), AF305540 (C. reinhardtii cox2b cDNA), AF305541 (Polytomella sp. genomic cox2a), AF305542 (Polytomella sp. genomic cox2b), and AF305543 (C. reinhardtii genomic cox2b).

** To whom correspondence should be addressed. Tel.: 011-525-622-5620; Fax: 011-525-622-5611 or 011-525-548-0387; E-mail: dhalphen@ifisiol.unam.mx.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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