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J. Biol. Chem., Vol. 276, Issue 15, 11531-11538, April 13, 2001
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From the Graduate Program in Genetics and Molecular Biology and
Department of Biochemistry, Emory University School of Medicine,
Atlanta, Georgia 30322
In vitro, transcript elongation by
RNA polymerase II is impeded by DNA sequences, DNA-bound proteins, and
small ligands. Transcription elongation factor SII (TFIIS) assists RNA
polymerase II to transcribe through these obstacles. There is however,
little direct evidence that SII-responsive arrest sites function in
living cells nor that SII facilitates readthrough in vivo.
Saccharomyces cerevisiae strains lacking elongation factor
SII and/or containing a point mutation in the second largest subunit of
RNA polymerase II, which slows the enzyme's RNA elongation rate, grow
slowly and have defects in mRNA metabolism, particularly in the
presence of nucleotide-depleting drugs. Here we have examined
transcriptional induction in strains lacking SII or containing the slow
polymerase mutation. Both mutants and a combined double mutant were
defective in induction of GAL1 and ENA1. This
was not due to an increase in mRNA degradation and was independent
of any drug treatment, although treatment with the nucleotide-depleting
drug 6-azauracil exacerbated the effect preferentially in the mutants.
These data are consistent with mutants in the Elongator complex, which
show slow inductive responses. When a potent in vitro
arrest site was transcribed in these strains, there was no perceptible
effect upon mRNA accumulation. These data suggest that an
alternative elongation surveillance mechanism exists in
vivo to overcome arrest.
To whom correspondence should be addressed: Dept. of Biochemistry,
Emory University School of Medicine, Rollins Research Center, 1510 Clifton Rd., Atlanta, GA 30322. Tel.: 404-727-3361; Fax: 404-727-3452;
E-mail: dreines@emory.edu.
This article has been cited by other articles:
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H.-J. Kim, S.-H. Jeong, J.-H. Heo, S.-J. Jeong, S.-T. Kim, H.-D. Youn, J.-W. Han, H.-W. Lee, and E.-J. Cho mRNA Capping Enzyme Activity Is Coupled to an Early Transcription Elongation Mol. Cell. Biol., July 15, 2004; 24(14): 6184 - 6193. [Abstract] [Full Text] [PDF]
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