|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 15, 11712-11718, April 13, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the In mammalian cells, intracellular sphingosine
1-phosphate (S1P) can stimulate calcium release from intracellular
organelles, resulting in the activation of downstream signaling
pathways. The budding yeast Saccharomyces cerevisiae
expresses enzymes that can synthesize and degrade S1P and related
molecules, but their possible role in calcium signaling has not yet
been tested. Here we examine the effects of S1P accumulation on
calcium signaling using a variety of yeast mutants. Treatment of yeast
cells with exogenous sphingosine stimulated Ca2+
accumulation through two distinct pathways. The first pathway required
the Cch1p and Mid1p subunits of a Ca2+ influx channel,
depended upon the function of sphingosine kinases (Lcb4p and Lcb5p),
and was inhibited by the functions of S1P lyase (Dpl1p) and the S1P
phosphatase (Lcb3p). The biologically inactive stereoisomer of
sphingosine did not activate this Ca2+ influx pathway,
suggesting that the active S1P isomer specifically stimulates a
calcium-signaling mechanism in yeast. The second Ca2+
influx pathway stimulated by the addition of sphingosine was not
stereospecific, was not dependent on the sphingosine kinases, occurred
only at higher doses of added sphingosine, and therefore was likely to
be nonspecific. Mutants lacking both S1P lyase and phosphatase
(dpl1 lcb3 double mutants) exhibited constitutively high
Ca2+ accumulation and signaling in the absence of added
sphingosine, and these effects were dependent on the sphingosine
kinases. These results show that endogenous S1P-related molecules can
also trigger Ca2+ accumulation and signaling. Several
stimuli previously shown to evoke calcium signaling in wild-type cells
were examined in lcb4 lcb5 double mutants. All of the
stimuli produced calcium signals independent of sphingosine kinase
activity, suggesting that phosphorylated sphingoid bases might serve as
messengers of calcium signaling in yeast during an unknown cellular response.
Calcium Influx and Signaling in Yeast Stimulated by Intracellular
Sphingosine 1-Phosphate Accumulation*
,
Department of Biology, Johns Hopkins
University, Baltimore, Maryland 21218, the § Children's
Hospital Oakland Research Institute, Oakland, California 94609, and the
¶ Department of Biochemistry, University of Kentucky College of
Medicine, Lexington, Kentucky 40536
*
This work was supported in part by the Searle Scholars
Program/The Chicago Community Trust (to K. W. C.) and National
Institutes of Health Grants CA77528 (to J. D. S.), GM41302 (to
R. C. D.), and GM53082 (to K. W. C.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Biology, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218. Tel.: 410-516-7844; Fax: 410-516-5213; E-mail: kwc@jhunix.hcf.jhu.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Morgan, P. McCourt, L. Rankin, E. Swain, L. M. Rice, and J. T. Nickels Jr. Altering Sphingolipid Metabolism in Saccharomyces cerevisiae Cells Lacking the Amphiphysin Ortholog Rvs161 Reinitiates Sugar Transporter Endocytosis Eukaryot. Cell, May 1, 2009; 8(5): 779 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mukhopadhyay, K. S. Howell, H. Riezman, and G. Capitani Identifying Key Residues of Sphinganine-1-phosphate Lyase for Function in Vivo and in Vitro J. Biol. Chem., July 18, 2008; 283(29): 20159 - 20169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ruiz and J. Arino Function and Regulation of the Saccharomyces cerevisiae ENA Sodium ATPase System Eukaryot. Cell, December 1, 2007; 6(12): 2175 - 2183. [Full Text] [PDF]
|
|
|
|
|
|
S. Iwaki, T. Sano, T. Takagi, M. Osumi, A. Kihara, and Y. Igarashi Intracellular Trafficking Pathway of Yeast Long-chain Base Kinase Lcb4, from Its Synthesis to Its Degradation J. Biol. Chem., September 28, 2007; 282(39): 28485 - 28492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Daquinag, M. Fadri, S. Y. Jung, J. Qin, and J. Kunz The Yeast PH Domain Proteins Slm1 and Slm2 Are Targets of Sphingolipid Signaling during the Response to Heat Stress Mol. Cell. Biol., January 15, 2007; 27(2): 633 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Brace, R. L. Lester, R. C. Dickson, and C. M. Rudin SVF1 Regulates Cell Survival by Affecting Sphingolipid Metabolism in Saccharomyces cerevisiae Genetics, January 1, 2007; 175(1): 65 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bultynck, V. L. Heath, A. P. Majeed, J.-M. Galan, R. Haguenauer-Tsapis, and M. S. Cyert Slm1 and Slm2 Are Novel Substrates of the Calcineurin Phosphatase Required for Heat Stress-Induced Endocytosis of the Yeast Uracil Permease Mol. Cell. Biol., June 15, 2006; 26(12): 4729 - 4745. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Naetzker, N. Hagen, and G. van Echten-Deckert Activation of p38 mitogen-activated protein kinase and partial reactivation of the cell cycle by cis-4-methylsphingosine direct postmitotic neurons towards apoptosis Genes Cells, March 1, 2006; 11(3): 269 - 279. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sano, A. Kihara, F. Kurotsu, S. Iwaki, and Y. Igarashi Regulation of the Sphingoid Long-chain Base Kinase Lcb4p by Ergosterol and Heme: STUDIES IN PHYTOSPHINGOSINE-RESISTANT MUTANTS J. Biol. Chem., November 4, 2005; 280(44): 36674 - 36682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Maceyka, H. Sankala, N. C. Hait, H. Le Stunff, H. Liu, R. Toman, C. Collier, M. Zhang, L. S. Satin, A. H. Merrill Jr., et al. SphK1 and SphK2, Sphingosine Kinase Isoenzymes with Opposing Functions in Sphingolipid Metabolism J. Biol. Chem., November 4, 2005; 280(44): 37118 - 37129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kihara, F. Kurotsu, T. Sano, S. Iwaki, and Y. Igarashi Long-Chain Base Kinase Lcb4 Is Anchored to the Membrane through Its Palmitoylation by Akr1 Mol. Cell. Biol., November 1, 2005; 25(21): 9189 - 9197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Iwaki, A. Kihara, T. Sano, and Y. Igarashi Phosphorylation by Pho85 Cyclin-dependent Kinase Acts as a Signal for the Down-regulation of the Yeast Sphingoid Long-chain Base Kinase Lcb4 during the Stationary Phase J. Biol. Chem., February 25, 2005; 280(8): 6520 - 6527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Germann, E. Swain, L. Bergman, and J. T. Nickels Jr. Characterizing the Sphingolipid Signaling Pathway That Remediates Defects Associated with Loss of the Yeast Amphiphysin-like Orthologs, Rvs161p and Rvs167p J. Biol. Chem., February 11, 2005; 280(6): 4270 - 4278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Viladevall, R. Serrano, A. Ruiz, G. Domenech, J. Giraldo, A. Barcelo, and J. Arino Characterization of the Calcium-mediated Response to Alkaline Stress in Saccharomyces cerevisiae J. Biol. Chem., October 15, 2004; 279(42): 43614 - 43624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Saba and T. Hla Point-Counterpoint of Sphingosine 1-Phosphate Metabolism Circ. Res., April 2, 2004; 94(6): 724 - 734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Reiss, B. Oskouian, J. Zhou, V. Gupta, P. Sooriyakumaran, S. Kelly, E. Wang, A. H. Merrill Jr., and J. D. Saba Sphingosine-phosphate Lyase Enhances Stress-induced Ceramide Generation and Apoptosis J. Biol. Chem., January 9, 2004; 279(2): 1281 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Uemura, A. Kihara, J.-i. Inokuchi, and Y. Igarashi Csg1p and Newly Identified Csh1p Function in Mannosylinositol Phosphorylceramide Synthesis by Interacting with Csg2p J. Biol. Chem., November 14, 2003; 278(46): 45049 - 45055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Itagaki and C. J. Hauser Sphingosine 1-Phosphate, a Diffusible Calcium Influx Factor Mediating Store-operated Calcium Entry J. Biol. Chem., July 18, 2003; 278(30): 27540 - 27547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kihara and Y. Igarashi Identification and Characterization of a Saccharomyces cerevisiae Gene, RSB1, Involved in Sphingoid Long-chain Base Release J. Biol. Chem., August 9, 2002; 277(33): 30048 - 30054. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Spiegel and S. Milstien Sphingosine 1-Phosphate, a Key Cell Signaling Molecule J. Biol. Chem., July 12, 2002; 277(29): 25851 - 25854. [Full Text] [PDF]
|
|
|
|
|
|
E. Swain, K. Baudry, J. Stukey, V. McDonough, M. Germann, and J. T. Nickels Jr. Sterol-dependent Regulation of Sphingolipid Metabolism in Saccharomyces cerevisiae J. Biol. Chem., July 12, 2002; 277(29): 26177 - 26184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bonilla and K. W. Cunningham Calcium Release and Influx in Yeast: TRPC and VGCC Rule Another Kingdom Sci. Signal., April 9, 2002; 2002(127): pe17 - pe17. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |