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J. Biol. Chem., Vol. 276, Issue 16, 12485-12488, April 20, 2001
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, and
From the Tsukuba Life Science Center, The Institute of
Physical and Chemical Research (RIKEN), Koyadai 3-1, Tsukuba, Ibaraki
305-0074, Japan
We isolated a novel gene termed
interleukin (IL)-1-inducible nuclear ankyrin-repeat protein
(INAP), of which expression was specifically induced by IL-1 in OP9
stromal cells. The INAP has ankyrin-repeat motifs and shares weak amino
acid sequence homology with Bcl-3 and other I
B family members. The
human genomic INAP gene found in the NCBI data base is located
at chromosome 3q3.11. Northern blot analyses revealed that INAP was not
expressed in any examined tissues without stimulation, but INAP
expression was rapidly and transiently induced by IL-1 although not by
tumor necrosis factor 
nor by phorbol 12-myristate 13-acetate in OP9 cells. Immunoblots with anti-INAP-specific antibody demonstrated that
INAP was rapidly and specifically produced by IL-1 stimulation and was
predominantly localized in the nucleus. Immunofluorescence stainings
showed that the INAP newly synthesized by IL-1 stimulation was promptly
translocated into the nucleus, and FLAG-tagged INAP forcibly expressed
in NIH/3T3 cells was also specifically localized in the nucleus. The
possible interaction of INAP with RelA/p65, NF-B1/p50, NF-B2/p52,
C/EBP
, and retinoid X receptor was examined, but we could detect
none of these interactions in the nuclear extracts of IL-1-stimulated
cells. Unlike Bcl-3 and other IB family members, INAP may play a
unique role in IL-1-induced specific gene expression and/or signal
transduction in the nucleus.
The nucleotide sequence(s) reported in this paper has been submitted to the DDBJ/GenBankTM/EBI Data Bank with accession number(s) AB026551.
Present address: Dept. of Biological Sciences, Tokyo Inst. of
Technology, Yokohama, Japan.
§
To whom correspondence should be addressed. Tel.: 81-298-36-9075;
Fax: 81-298-36-9090; E-mail: todokoro@rtc.riken.go.jp.
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