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J. Biol. Chem., Vol. 276, Issue 16, 12513-12519, April 20, 2001
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From the Unidad de Biofísica (Consejo Superior de
Investigaciones Científicas-UPV/EHU), and Departamento de
Bioquímica, Universidad del País Vasco/Euskal Herriko
Unibertsitatea, Aptdo. 644, Bilbao 48080, Spain
Escherichia coli
Glycophorin as a Receptor for Escherichia coli
-Hemolysin in Erythrocytes*
,
-hemolysin (HlyA)
can lyse both red blood cells (RBC) and liposomes. However, the cells
are lysed at HlyA concentrations 1-2 orders of magnitude lower than
liposomes (large unilamellar vesicles). Treatment of RBC with trypsin,
but not with chymotrypsin, reduces the sensitivity of RBC toward HlyA to the level of the liposomes. Since glycophorin, one of the main proteins in the RBC surface, can be hydrolyzed by trypsin much more
readily than by chymotrypsin, the possibility was tested of a specific
binding of HlyA to glycophorin. With this purpose, a number of
experiments were performed. (a) HlyA was preincubated with
purified glycophorin, after which it was found to be inactive against
both RBC and liposomes. (b) Treatment of RBC with an
anti-glycophorin antibody protected the cells against HlyA lysis.
(c) Immobilized HlyA was able to bind glycophorin present
in a detergent lysate of RBC ghosts. (d) Incorporation of
glycophorin into pure phosphatidylcholine liposomes increased
notoriously the sensitivity of the vesicles toward HlyA.
(e) Treatment of the glycophorin-containing liposomes with
trypsin reverted the vesicles to their original low sensitivity. The
above results are interpreted in terms of glycophorin acting as a
receptor for HlyA in RBC. The binding constant of HlyA for glycophorin
was estimated, in RBC at sublytic HlyA concentrations, to be 1.5 × 10
9 M.
*
This work was supported by Direccion General de
Investigacion Cientifica y Tecnica Grant PB96-0171, Basque Government
Grant EX99/05, and University of the Basque Country Grant G03/98.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of a fellowship from the Basque government.
§
To whom correspondence should be addressed. Tel.: 34-94-6012542;
Fax: 34-94-4648850; E-mail: gbzoseth@lg.ehu.es.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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