|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 16, 12645-12653, April 20, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Molecular and Cellular Pharmacology,
University of Miami Medical Center, Miami, Florida 33149
Endothelin-1 (ET-1) is a peptide hormone
with potent vasoconstrictor properties which is synthesized and
secreted predominantly by vascular endothelial cells. Its production is
regulated by numerous stimuli including ischemia and hypoxia, and the
enhanced levels that occur during myocardial ischemia may contribute to the progression of heart failure. We reported previously a preliminary characterization of a hypoxia-inducible factor-1 (HIF-1) binding site
in the human ET-1 promoter which contributed to the activation of ET-1
expression in endothelial cells. We report here that the HIF-1 binding
site alone is not sufficient for the response to hypoxia but requires
an additional 50 base pairs of flanking sequence that includes binding
sites for the factors activator protein-1 (AP-1), GATA-2, and
CAAT-binding factor (NF-1). Mutation of any one of these sites or the
HIF-1 site eliminated induction by hypoxia. Mutations of the AP-1 and
GATA-2 sites, but not the HIF-1 site, were complemented by
overexpressing AP-1, GATA-2, HIF-1
Molecular Regulation of the Endothelin-1 Gene by
Hypoxia
CONTRIBUTIONS OF HYPOXIA-INDUCIBLE FACTOR-1, ACTIVATOR
PROTEIN-1, GATA-2, AND p300/CBP*
, or the activator protein
p300/CBP, restoring the response to hypoxia. Binding studies in
vitro confirmed physical associations among GATA-2, AP-1, and
HIF-1 factors. Overexpression or depletion of p300/CBP modulated the
level of ET-1 promoter expression as well as the endogenous ET-1
transcript but did not change the fold induction by hypoxia in either
case. Regulation of the ET-1 promoter by hypoxia in
non-endothelial cells required overexpression of GATA-2 and HIF-1.
The results support essential roles for AP-1, GATA-2, and NF-1 in
stabilizing the binding of HIF-1 and promoting recruitment of p300/CBP
to the ET-1 hypoxia response complex.
*
This work was supported by Grant HL44578 from the National
Institutes of Health (to K. A. W.) and by a postdoctoral fellowship from the American Heart Association, Florida Affiliate (to K. Y.). A
preliminary report of this work has been published in abstract form
(Yamashita, K., Discher, D. J., Hu, J., Bishopric, N. H., and
Webster, K. A. (1999) Circulation 98, 74).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Molecular and
Cellular Pharmacology, Rosenstiel Medical Science Bldg., Rm. 6038, University of Miami Medical Center, 1600 NW 10th Ave., Miami, FL
33136. Tel.: 305-243-6779; Fax: 305-243-3082; E-mail: kwebster@chroma.med.miami.edu.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Belaidi, M. Joyeux-Faure, C. Ribuot, S. H. Launois, P. Levy, and D. Godin-Ribuot Major role for hypoxia inducible factor-1 and the endothelin system in promoting myocardial infarction and hypertension in an animal model of obstructive sleep apnea. J. Am. Coll. Cardiol., April 14, 2009; 53(15): 1309 - 1317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Peled, A. Shaish, A. Katav, S. Greenberger, I. Barshack, R. Tal, L. Bangio, E. Breitbart, and D. Harats Systemic Administration of a Conditionally Replicating Adenovirus, Targeted to Angiogenesis, Reduced Lung Metastases Burden in Cotton Rats Clin. Cancer Res., March 1, 2009; 15(5): 1664 - 1673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. O. Leonard, K. Howell, S. F. Madden, C. M. Costello, D. G. Higgins, C. T. Taylor, and P. McLoughlin Hypoxia Selectively Activates the CREB Family of Transcription Factors in the In Vivo Lung Am. J. Respir. Crit. Care Med., November 1, 2008; 178(9): 977 - 983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. N. Ivey, D. Sutcliffe, J. Richardson, R. I. Clyman, J. A. Garcia, and D. Srivastava Transcriptional Regulation During Development of the Ductus Arteriosus Circ. Res., August 15, 2008; 103(4): 388 - 395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Patel, C. S. Gonsalves, P. Malik, and V. K. Kalra Placenta growth factor augments endothelin-1 and endothelin-B receptor expression via hypoxia-inducible factor-1{alpha} Blood, August 1, 2008; 112(3): 856 - 865. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Gomez, M. J. Moreno, R. M. Baldrich, and A. Hernandez Endothelin-1 Molecular Ribonucleic Acid Expression in Pulmonary Hypertensive and Nonhypertensive Chickens Poult. Sci., July 1, 2008; 87(7): 1395 - 1401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. de Frutos, L. Duling, D. Alo, T. Berry, O. Jackson-Weaver, M. Walker, N. Kanagy, and L. Gonzalez Bosc NFATc3 is required for intermittent hypoxia-induced hypertension Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2382 - H2390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Lund, L. N. Agbor, N. Zhang, A. Baker, H. Zhao, G. D. Fink, N. L. Kanagy, and M. K. Walker Loss of the Aryl Hydrocarbon Receptor Induces Hypoxemia, Endothelin-1, and Systemic Hypertension at Modest Altitude Hypertension, March 1, 2008; 51(3): 803 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Ribatti, M. T. Conconi, and G. G. Nussdorfer Nonclassic Endogenous Novel Regulators of Angiogenesis Pharmacol. Rev., June 1, 2007; 59(2): 185 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Khandekar, W. Brandt, Y. Zhou, S. Dagenais, T. W. Glover, N. Suzuki, R. Shimizu, M. Yamamoto, K.-C. Lim, and J. D. Engel A Gata2 intronic enhancer confers its pan-endothelia-specific regulation Development, May 1, 2007; 134(9): 1703 - 1712. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Chauhan, S. Hahn, S. Gartner, C. A. Pardo, S. K. Netesan, J. McArthur, and A. Nath Molecular programming of endothelin-1 in HIV-infected brain: role of Tat in up-regulation of ET-1 and its inhibition by statins FASEB J, March 1, 2007; 21(3): 777 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Patiar and A. L Harris Role of hypoxia-inducible factor-1{alpha} as a cancer therapy target Endocr. Relat. Cancer, December 1, 2006; 13(Supplement_1): S61 - S75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Luo, C. Jiang, A. J. Belanger, G. Y. Akita, S. C. Wadsworth, R. J. Gregory, and K. A. Vincent A Constitutively Active Hypoxia-Inducible Factor-1{alpha}/VP16 Hybrid Factor Activates Expression of the Human B-Type Natriuretic Peptide Gene Mol. Pharmacol., June 1, 2006; 69(6): 1953 - 1962. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Miyamoto, T. Kawamura, T. Morimoto, K. Ono, H. Wada, Y. Kawase, A. Matsumori, R. Nishio, T. Kita, and K. Hasegawa Histone Acetyltransferase Activity of p300 Is Required for the Promotion of Left Ventricular Remodeling After Myocardial Infarction in Adult Mice In Vivo Circulation, February 7, 2006; 113(5): 679 - 690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kobayashi, H. Oku, M. Fukuhara, S. Kojima, A. Komori, M. Ichikawa, K. Katsumura, M. Kobayashi, T. Sugiyama, and T. Ikeda Endothelin-1 Enhances Glutamate-Induced Retinal Cell Death, Possibly through ETA Receptors Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4684 - 4690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Carpenter, S. Schomberg, and K. R. Stenmark Endothelin-mediated increases in lung VEGF content promote vascular leak in young rats exposed to viral infection and hypoxia Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L1075 - L1082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wu, F. Iwata, J. A. Grass, C. S. Osborne, L. Elnitski, P. Fraser, O. Ohneda, M. Yamamoto, and E. H. Bresnick Molecular Determinants of NOTCH4 Transcription in Vascular Endothelium Mol. Cell. Biol., February 15, 2005; 25(4): 1458 - 1474. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Govindaraju, H. Teoh, Q. Hamid, P. Cernacek, and M. E. Ward Interaction between endothelial heme oxygenase-2 and endothelin-1 in altered aortic reactivity after hypoxia in rats Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H962 - H970. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B. Waypa and P. T. Schumacker Hypoxic pulmonary vasoconstriction: redox events in oxygen sensing J Appl Physiol, January 1, 2005; 98(1): 404 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. G von Lueder, H. Kjekshus, T. Edvardsen, E. Oie, S. Urheim, L. E. Vinge, M. S. Ahmed, O. A Smiseth, and H. Attramadal Mechanisms of elevated plasma endothelin-1 in CHF: congestion increases pulmonary synthesis and secretion of endothelin-1 Cardiovasc Res, July 1, 2004; 63(1): 41 - 50. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Seki, K.-H. Hong, J. Yun, S.-J. Kim, and S. P. Oh Isolation of a Regulatory Region of Activin Receptor-Like Kinase 1 Gene Sufficient for Arterial Endothelium-Specific Expression Circ. Res., April 30, 2004; 94(8): e72 - e77. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Hopfl, O. Ogunshola, and M. Gassmann HIFs and tumors--causes and consequences Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2004; 286(4): R608 - R623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pichiule, J. C. Chavez, and J. C. LaManna Hypoxic Regulation of Angiopoietin-2 Expression in Endothelial Cells J. Biol. Chem., March 26, 2004; 279(13): 12171 - 12180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Tai, G. B. Robb, and P. A. Marsden Endothelial Nitric Oxide Synthase: A New Paradigm for Gene Regulation in the Injured Blood Vessel Arterioscler. Thromb. Vasc. Biol., March 1, 2004; 24(3): 405 - 412. [Abstract] [Full Text]
|
|
|
|
|
|
K. A. Seta and D. E. Millhorn Functional genomics approach to hypoxia signaling J Appl Physiol, February 1, 2004; 96(2): 765 - 773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. LaVoie The Role of GATA in Mammalian Reproduction Experimental Biology and Medicine, December 1, 2003; 228(11): 1282 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Coulet, S. Nadaud, M. Agrapart, and F. Soubrier Identification of Hypoxia-response Element in the Human Endothelial Nitric-oxide Synthase Gene Promoter J. Biol. Chem., November 21, 2003; 278(47): 46230 - 46240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yim, S. M. Choi, Y. Choi, N. Lee, J. Chung, and H. Park Insulin and Hypoxia Share Common Target Genes but Not the Hypoxia-inducible Factor-1{alpha} J. Biol. Chem., October 3, 2003; 278(40): 38260 - 38268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sorokin and D. E. Kohan Physiology and pathology of endothelin-1 in renal mesangium Am J Physiol Renal Physiol, October 1, 2003; 285(4): F579 - F589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Webster Evolution of the coordinate regulation of glycolytic enzyme genes by hypoxia J. Exp. Biol., September 1, 2003; 206(17): 2911 - 2922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Li, S. Oparil, J.-Z. Sun, J. A. Thompson, and Y.-F. Chen Fibroblast growth factor mediates hypoxia-induced endothelin-A receptor expression in lung artery smooth muscle cells J Appl Physiol, August 1, 2003; 95(2): 643 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Rodriguez-Pascual, M. Redondo-Horcajo, and S. Lamas Functional Cooperation Between Smad Proteins and Activator Protein-1 Regulates Transforming Growth Factor-{beta}-Mediated Induction of Endothelin-1 Expression Circ. Res., June 27, 2003; 92(12): 1288 - 1295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Favier, P.-F. Plouin, P. Corvol, and J.-M. Gasc Angiogenesis and Vascular Architecture in Pheochromocytomas : Distinctive Traits in Malignant Tumors Am. J. Pathol., October 1, 2002; 161(4): 1235 - 1246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Adamy, P. Oliviero, S. Eddahibi, L. Rappaport, J.-L. Samuel, E. Teiger, and C. Chassagne Cardiac modulations of ANG II receptor expression in rats with hypoxic pulmonary hypertension Am J Physiol Heart Circ Physiol, August 1, 2002; 283(2): H733 - H740. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Spinella, L. Rosano, V. Di Castro, P. G. Natali, and A. Bagnato Endothelin-1 Induces Vascular Endothelial Growth Factor by Increasing Hypoxia-inducible Factor-1alpha in Ovarian Carcinoma Cells J. Biol. Chem., July 26, 2002; 277(31): 27850 - 27855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Earley and T. C. Resta Estradiol attenuates hypoxia-induced pulmonary endothelin-1 gene expression Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L86 - L93. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H. J. Pannen New Insights Into the Regulation of Hepatic Blood Flow After Ischemia and Reperfusion Anesth. Analg., June 1, 2002; 94(6): 1448 - 1457. [Full Text] [PDF]
|
|
|
|
|
|
S. Earley, L. D. Nelin, L. G. Chicoine, and B. R. Walker Hypoxia-induced pulmonary endothelin-1 expression is unaltered by nitric oxide J Appl Physiol, March 1, 2002; 92(3): 1152 - 1158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Alfranca, M. D. Gutierrez, A. Vara, J. Aragones, F. Vidal, and M. O. Landazuri c-Jun and Hypoxia-Inducible Factor 1 Functionally Cooperate in Hypoxia-Induced Gene Transcription Mol. Cell. Biol., January 1, 2002; 22(1): 12 - 22. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Aitsebaomo, M. L. Kingsley-Kallesen, Y. Wu, T. Quertermous, and C. Patterson Vezf1/DB1 Is an Endothelial Cell-specific Transcription Factor That Regulates Expression of the Endothelin-1 Promoter J. Biol. Chem., October 12, 2001; 276(42): 39197 - 39205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Kline, Y.-J. Peng, D. J. Manalo, G. L. Semenza, and N. R. Prabhakar Defective carotid body function and impaired ventilatory responses to chronic hypoxia in mice partially deficient for hypoxia-inducible factor 1alpha PNAS, January 22, 2002; 99(2): 821 - 826. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |