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Originally published In Press as doi:10.1074/jbc.M100501200 on January 24, 2001

J. Biol. Chem., Vol. 276, Issue 16, 12764-12768, April 20, 2001
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Effects of Histone Acetylation on the Solubility and Folding of the Chromatin Fiber*

Xiaoying Wang, Cheng He, Susan C. Moore, and Juan AusióDagger

From the Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia V8W 3P6, Canada

The folding ability of chromatin fractions containing approximately identical nucleosome numbers and the same linker histone composition, but with different extents of core histone acetylation, were analyzed by analytical ultracentrifugation. It was found that the acetylated fractions consistently exhibited a relatively small but significantly lower extent of compaction than that of their native nonacetylated counterparts. This was regardless of the extent of the size distribution heterogeneity of the fractions analyzed. Furthermore the acetylated chromatin fibers exhibited an enhanced solubility in both NaCl and MgCl2, which is neither the result of a differential binding affinity of the linker histones to chromatin nor of an alteration in the relative amounts of the histone H1 variants.

* This work was supported by Medical Research Council of Canada Grant MT-13104 (to J. A.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Biochemistry and Microbiology, University of Victoria, P. O. Box 3055, Petch Bldg., 220, Victoria, British Columbia V8W 3P6, Canada. Tel.: 250-721-8863; Fax: 250-721-8855; E-mail: jausio@uvic.ca.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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