JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/jbc.M010423200 on January 22, 2001

J. Biol. Chem., Vol. 276, Issue 16, 13198-13208, April 20, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/16/13198    most recent
M010423200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ma, Z.
Right arrow Articles by Turk, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ma, Z.
Right arrow Articles by Turk, J.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Studies of Insulin Secretory Responses and of Arachidonic Acid Incorporation into Phospholipids of Stably Transfected Insulinoma Cells That Overexpress Group VIA Phospholipase A2 (iPLA2beta ) Indicate a Signaling Rather Than a Housekeeping Role for iPLA2beta *

Zhongmin Ma, Sasanka Ramanadham, Mary Wohltmann, Alan Bohrer, Fong-Fu Hsu, and John TurkDagger

From the Mass Spectrometry Resource, Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

A cytosolic 84-kDa group VIA phospholipase A2 (iPLA2beta ) that does not require Ca2+ for catalysis has been cloned from several sources, including rat and human pancreatic islet beta -cells and murine P388D1 cells. Many potential iPLA2beta functions have been proposed, including a signaling role in beta -cell insulin secretion and a role in generating lysophosphatidylcholine acceptors for arachidonic acid incorporation into P388D1 cell phosphatidylcholine (PC). Proposals for iPLA2beta function rest in part on effects of inhibiting iPLA2beta activity with a bromoenol lactone (BEL) suicide substrate, but BEL also inhibits phosphatidate phosphohydrolase-1 and a group VIB phospholipase A2. Manipulation of iPLA2beta expression by molecular biologic means is an alternative approach to study iPLA2beta functions, and we have used a retroviral construct containing iPLA2beta cDNA to prepare two INS-1 insulinoma cell clonal lines that stably overexpress iPLA2beta . Compared with parental INS-1 cells or cells transfected with empty vector, both iPLA2beta -overexpressing lines exhibit amplified insulin secretory responses to glucose and cAMP-elevating agents, and BEL substantially attenuates stimulated secretion. Electrospray ionization mass spectrometric analyses of arachidonic acid incorporation into INS-1 cell PC indicate that neither overexpression nor inhibition of iPLA2beta affects the rate or extent of this process in INS-1 cells. Immunocytofluorescence studies with antibodies directed against iPLA2beta indicate that cAMP-elevating agents increase perinuclear fluorescence in INS-1 cells, suggesting that iPLA2beta associates with nuclei. These studies are more consistent with a signaling than with a housekeeping role for iPLA2beta in insulin-secreting beta -cells.


* This work was supported by National Institutes of Health Grants R37-DK-34388, P41-RR00954, PO1-HL57278, P60-DK20579, and P30-DK56341; by Career Development Award 2-1999-55 (to Z. M.) from the Juvenile Diabetes Foundation; and by a grant from the American Diabetes Association.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Box 8127, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, MO 63110. Tel.: 314-362-8190; Fax: 314-362-8188; E-mail: jturk@imgate.wustl.edu.


Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Am. J. Physiol. Endocrinol. Metab.Home page
S. Bao, D. A. Jacobson, M. Wohltmann, A. Bohrer, W. Jin, L. H. Philipson, and J. Turk
Glucose homeostasis, insulin secretion, and islet phospholipids in mice that overexpress iPLA2{beta} in pancreatic {beta}-cells and in iPLA2{beta}-null mice
Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E217 - E229.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Endocrinol. Metab.Home page
V. Poitout
Phospholipid hydrolysis and insulin secretion: a step toward solving the Rubik's cube
Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E214 - E216.
[Full Text] [PDF]


Home page
J. Cell Sci.Home page
X. H. Zhang, C. Zhao, and Z. A. Ma
The increase of cell-membranous phosphatidylcholines containing polyunsaturated fatty acid residues induces phosphorylation of p53 through activation of ATR
J. Cell Sci., December 1, 2007; 120(23): 4134 - 4143.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Bao, Y. Li, X. Lei, M. Wohltmann, W. Jin, A. Bohrer, C. F. Semenkovich, S. Ramanadham, I. Tabas, and J. Turk
Attenuated Free Cholesterol Loading-induced Apoptosis but Preserved Phospholipid Composition of Peritoneal Macrophages from Mice That Do Not Express Group VIA Phospholipase A2
J. Biol. Chem., September 14, 2007; 282(37): 27100 - 27114.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
D. A. Jacobson, C. R. Weber, S. Bao, J. Turk, and L. H. Philipson
Modulation of the Pancreatic Islet beta-Cell-delayed Rectifier Potassium Channel Kv2.1 by the Polyunsaturated Fatty Acid Arachidonate
J. Biol. Chem., March 9, 2007; 282(10): 7442 - 7449.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
K. Seleznev, C. Zhao, X. H. Zhang, K. Song, and Z. A. Ma
Calcium-independent Phospholipase A2 Localizes in and Protects Mitochondria during Apoptotic Induction by Staurosporine
J. Biol. Chem., August 4, 2006; 281(31): 22275 - 22288.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Bao, H. Song, M. Wohltmann, S. Ramanadham, W. Jin, A. Bohrer, and J. Turk
Insulin Secretory Responses and Phospholipid Composition of Pancreatic Islets from Mice That Do Not Express Group VIA Phospholipase A2 and Effects of Metabolic Stress on Glucose Homeostasis
J. Biol. Chem., July 28, 2006; 281(30): 20958 - 20973.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. M. Jenkins, W. Yan, D. J. Mancuso, and R. W. Gross
Highly Selective Hydrolysis of Fatty Acyl-CoAs by Calcium-independent Phospholipase A2beta: ENZYME AUTOACYLATION AND ACYL-CoA-MEDIATED REVERSAL OF CALMODULIN INHIBITION OF PHOSPHOLIPASE A2 ACTIVITY
J. Biol. Chem., June 9, 2006; 281(23): 15615 - 15624.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Bao, A. Bohrer, S. Ramanadham, W. Jin, S. Zhang, and J. Turk
Effects of Stable Suppression of Group VIA Phospholipase A2 Expression on Phospholipid Content and Composition, Insulin Secretion, and Proliferation of INS-1 Insulinoma Cells
J. Biol. Chem., January 6, 2006; 281(1): 187 - 198.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. M. Moran, R. M. L. Buller, J. McHowat, J. Turk, M. Wohltmann, R. W. Gross, and J. A. Corbett
Genetic and Pharmacologic Evidence That Calcium-independent Phospholipase A2{beta} Regulates Virus-induced Inducible Nitric-oxide Synthase Expression by Macrophages
J. Biol. Chem., July 29, 2005; 280(30): 28162 - 28168.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
Z. Wang, S. Ramanadham, Z. A. Ma, S. Bao, D. J. Mancuso, R. W. Gross, and J. Turk
Group VIA Phospholipase A2 Forms a Signaling Complex with the Calcium/Calmodulin-dependent Protein Kinase II{beta} Expressed in Pancreatic Islet {beta}-Cells
J. Biol. Chem., February 25, 2005; 280(8): 6840 - 6849.
[Abstract] [Full Text] [PDF]


Home page
Mol. Endocrinol.Home page
K. Song, X. Zhang, C. Zhao, N. T. Ang, and Z. A. Ma
Inhibition of Ca2+-Independent Phospholipase A2 Results in Insufficient Insulin Secretion and Impaired Glucose Tolerance
Mol. Endocrinol., February 1, 2005; 19(2): 504 - 515.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
R. Perez, R. Melero, M. A. Balboa, and J. Balsinde
Role of Group VIA Calcium-independent Phospholipase A2 in Arachidonic Acid Release, Phospholipid Fatty Acid Incorporation, and Apoptosis in U937 Cells Responding to Hydrogen Peroxide
J. Biol. Chem., September 24, 2004; 279(39): 40385 - 40391.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. Bao, D. J. Miller, Z. Ma, M. Wohltmann, G. Eng, S. Ramanadham, K. Moley, and J. Turk
Male Mice That Do Not Express Group VIA Phospholipase A2 Produce Spermatozoa with Impaired Motility and Have Greatly Reduced Fertility
J. Biol. Chem., September 10, 2004; 279(37): 38194 - 38200.
[Abstract] [Full Text] [PDF]


Home page
DiabetesHome page
P. M. Jones, C. J. Burns, V. D. Belin, H. M. Roderigo-Milne, and S. J. Persaud
The Role of Cytosolic Phospholipase A2 in Insulin Secretion
Diabetes, February 1, 2004; 53(90001): S172 - 178.
[Abstract] [Full Text]


Home page
DiabetesHome page
S. Ramanadham, H. Song, S. Bao, F.-F. Hsu, S. Zhang, Z. Ma, C. Jin, and J. Turk
Islet Complex Lipids: Involvement in the Actions of Group VIA Calcium-Independent Phospholipase A2 in {beta}-Cells
Diabetes, February 1, 2004; 53(90001): S179 - 185.
[Abstract] [Full Text]


Home page
DiabetesHome page
S. Bao, C. Jin, S. Zhang, J. Turk, Z. Ma, and S. Ramanadham
{beta}-Cell Calcium-Independent Group VIA Phospholipase A2 (iPLA2{beta}): Tracking iPLA2{beta} Movements in Response to Stimulation With Insulin Secretagogues in INS-1 Cells
Diabetes, February 1, 2004; 53(90001): S186 - 189.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
D. Arnette, T. B. Gibson, M. C. Lawrence, B. January, S. Khoo, K. McGlynn, C. A. Vanderbilt, and M. H. Cobb
Regulation of ERK1 and ERK2 by Glucose and Peptide Hormones in Pancreatic {beta} Cells
J. Biol. Chem., August 29, 2003; 278(35): 32517 - 32525.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
Z. Guo, W. Su, Z. Ma, G. M. Smith, and M. C. Gong
Ca2+-independent Phospholipase A2 Is Required for Agonist-induced Ca2+ Sensitization of Contraction in Vascular Smooth Muscle
J. Biol. Chem., January 10, 2003; 278(3): 1856 - 1863.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
L. B. Maggi Jr., J. M. Moran, A. L. Scarim, D. A. Ford, J.-W. Yoon, J. McHowat, R. M. L. Buller, and J. A. Corbett
Novel Role for Calcium-independent Phospholipase A2 in the Macrophage Antiviral Response of Inducible Nitric-oxide Synthase Expression
J. Biol. Chem., October 4, 2002; 277(41): 38449 - 38455.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. M. Jenkins, X. Han, D. J. Mancuso, and R. W. Gross
Identification of Calcium-independent Phospholipase A2 (iPLA2) beta , and Not iPLA2gamma , as the Mediator of Arginine Vasopressin-induced Arachidonic Acid Release in A-10 Smooth Muscle Cells. ENANTIOSELECTIVE MECHANISM-BASED DISCRIMINATION OF MAMMALIAN iPLA2s
J. Biol. Chem., August 30, 2002; 277(36): 32807 - 32814.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Endocrinol. Metab.Home page
Z. Ma, S. Zhang, J. Turk, and S. Ramanadham
Stimulation of insulin secretion and associated nuclear accumulation of iPLA2beta in INS-1 insulinoma cells
Am J Physiol Endocrinol Metab, April 1, 2002; 282(4): E820 - E833.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.