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J. Biol. Chem., Vol. 276, Issue 17, 13875-13880, April 27, 2001
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From the Elastase activity of Vibrio
vulnificus was highly dependent on growth phase, reached a
maximum during the stationary phase, and was regulated at the level of
transcription. The stationary phase production of elastase in
crp or rpoS mutants, which were constructed by
allelic exchanges, decreased about 3- and 10-fold, respectively.
However, the promoter activity of vvpE encoding elastase
was unaffected by those mutations in the log phase when analyzed using
a vvpE-lux fusion. A primer extension analysis revealed
that the transcription of vvpE begins at two different sites, consisting of putative promoter L (PL) and promoter S (PS). The
PL activity was constitutive through the log and stationary phases,
lower than the PS activity, and unaffected by the crp or
rpoS mutations. The transcription of PS, induced only in
the stationary phase, was dependent on RpoS. The mutation in
crp reduced the activity of PS; however, the additional
inactivation of crp did not influence the PS activity in
the rpoS mutant, indicating that CRP exerted its effects
through PS requiring RpoS. These results demonstrate that
vvpE expression is differentially directed by PL and PS
depending on the growth phase and elevated by RpoS and CRP in the
stationary phase.
Differential Expression of Vibrio vulnificus Elastase
Gene in a Growth Phase-dependent Manner by Two Different
Types of Promoters*
,,,
Department of Food Science and Technology,
Department of Molecular Biotechnology, Institute of Biotechnology,
Chonnam National University, Kwang-Ju 500-757, the
§ Department of Environmental Science, Hankuk University of
Foreign Studies, Yongin, Kyunggi-Do 449-791, and the ¶ Department
of Microbiology, Chonnam National University Medical School,
Kwang-Ju 500-190, South Korea
*
This work was supported by a grant to S. H. C. from the
Korea Research Foundation (2000-041-G00101), Republic of Korea.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Food
Science and Technology, Inst. of Biotechnology, Chonnam National University, Kwang-Ju 500-757, S. Korea. Tel.: 82-62-530-2146; Fax:
82-62-530-2149; E-mail: shchoi@chonnam.chonnam.ac.kr.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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