HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

J. Biol. Chem., Vol. 276, Issue 17, 13917-13923, April 27, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/17/13917    most recent M007745200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Seth, K. A. Articles by Majzoub, J. A. Search for Related Content PubMed PubMed Citation Articles by Seth, K. A. Articles by Majzoub, J. A. Social Bookmarking                      What's this?

Repressor Element Silencing Transcription Factor/Neuron-restrictive Silencing Factor (REST/NRSF) Can Act as an Enhancer as Well as a Repressor of Corticotropin-releasing Hormone Gene Transcription^*

Kim A. Seth^§ and Joseph A. Majzoub^§¶

From the  Program in Neuroscience, Harvard Medical School, and the ^§ Division of Endocrinology, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115

The repressor element-1/neuron-restrictive silencing element (RE-1/NRSE) mediates transcriptional repression by the repressor element silencing transcription factor/neuron-restrictive silencing factor (REST/NRSF) in many neuron-specific genes. REST/NRSF is expressed most highly in non-neural tissues, where it is thought to repress gene transcription, but is also found in developing neurons and at low levels in the brain. Its null mutation in vivo results in embryonic lethality in mice. While the RE-1/NRSE-mediated repressive influence of REST/NRSF is well established, results in transgenic studies have suggested that the action of the system is more complex. Here, we report that transcription of the corticotropin releasing hormone (CRH) gene is regulated by REST/NRSF, in part through the RE-1/NRSE. Expression of transfected Crh-luciferase constructs was down-regulated by REST/NRSF in a RE-1/NRSE-dependent fashion in both muscle-derived L6 and REST/NRSF co-transfected neuronal PC12 cells. Treatment of L6 cells with trichostatin A revealed that REST/NRSF repression depends, in part, on histone deacetylase activity in these cells. In another neuronal cell line, NG108, REST/NRSF also repressed expression from constructs containing an intact RE-1/NRSE. However, unexpectedly, REST/NRSF up-regulated expression levels of constructs lacking an intact RE-1/NRSE. These results suggest that REST/NRSF can act as both a repressor of Crh transcription, via the Crh RE-1/NRSE, and an enhancer of Crh transcription, via a mechanism independent of the Crh RE-1/NRSE.

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				J. A Majzoub Corticotropin-releasing hormone physiology Eur. J. Endocrinol., November 1, 2006; 155(suppl_1): S71 - S76. [Abstract] [Full Text] [PDF]

				C. Conaco, S. Otto, J.-J. Han, and G. Mandel Reciprocal actions of REST and a microRNA promote neuronal identity PNAS, February 14, 2006; 103(7): 2422 - 2427. [Abstract] [Full Text] [PDF]

				M. Czesak, S. Lemonde, E. A. Peterson, A. Rogaeva, and P. R. Albert Cell-Specific Repressor or Enhancer Activities of Deaf-1 at a Serotonin 1A Receptor Gene Polymorphism J. Neurosci., February 8, 2006; 26(6): 1864 - 1871. [Abstract] [Full Text] [PDF]

				M. Qiang, C. S. S. Rani, and M. K. Ticku Neuron-Restrictive Silencer Factor Regulates the N-Methyl-D-aspartate Receptor 2B Subunit Gene in Basal and Ethanol-Induced Gene Expression in Fetal Cortical Neurons Mol. Pharmacol., June 1, 2005; 67(6): 2115 - 2125. [Abstract] [Full Text] [PDF]

				C. S. Kim, C. K. Hwang, H. S. Choi, K. Y. Song, P.-Y. Law, L.-N. Wei, and H. H. Loh Neuron-restrictive Silencer Factor (NRSF) Functions as a Repressor in Neuronal Cells to Regulate the {micro} Opioid Receptor Gene J. Biol. Chem., November 5, 2004; 279(45): 46464 - 46473. [Abstract] [Full Text] [PDF]

				N. D. Belyaev, I. C. Wood, A. W. Bruce, M. Street, J.-B. Trinh, and N. J. Buckley Distinct RE-1 Silencing Transcription Factor-containing Complexes Interact with Different Target Genes J. Biol. Chem., January 2, 2004; 279(1): 556 - 561. [Abstract] [Full Text] [PDF]

				D. M. Kemp, J. C. Lin, and J. F. Habener Regulation of Pax4 Paired Homeodomain Gene by Neuron-restrictive Silencer Factor J. Biol. Chem., September 12, 2003; 278(37): 35057 - 35062. [Abstract] [Full Text] [PDF]

				R. Armisen, R. Fuentes, P. Olguin, M. E. Cabrejos, and M. Kukuljan Repressor Element-1 Silencing Transcription/Neuron-Restrictive Silencer Factor Is Required for Neural Sodium Channel Expression during Development of Xenopus J. Neurosci., October 1, 2002; 22(19): 8347 - 8351. [Abstract] [Full Text] [PDF]

				A. Roopra, Y. Huang, and R. Dingledine Neurological Disease: Listening to Gene Silencers Mol. Interv., October 1, 2001; 1(4): 219 - 228. [Abstract] [Full Text] [PDF]