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J. Biol. Chem., Vol. 276, Issue 17, 14289-14298, April 27, 2001
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From the We analyzed three chromosomal loci of the
trypanosomatid Leptomonas collosoma encoding box C/D small
nucleolar RNAs (snoRNAs). All the snoRNAs that were analyzed here carry
two sequences complementary to rRNA target sites and obey the +5 rule
for guide methylation. Studies on transgenic parasites carrying the
snoRNA-2 gene in the episomal expression vector (pX-neo)
indicated that no promoter activity was found immediately adjacent to
this gene. Deleting the flanking sequences of snoRNA-2 affected the
expression; in the absence of the 3'-flanking (but not 5'-flanking)
sequence, the expression was almost completely abolished. The snoRNA
genes are transcribed as polycistronic RNA. All snoRNAs can be folded into a common stem-loop structure, which may play a role in processing the polycistronic transcript. snoRNA B2, a member of a snoRNA cluster,
was expressed when cloned into the episomal vector, suggesting that
each gene within a cluster is individually processed. Studies with
permeable cells indicated that snoRNA gene transcription was relatively
sensitive to The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF331656
Expression Studies on Clustered Trypanosomatid Box C/D Small
Nucleolar RNAs*
§,
Department of Biological Chemistry, Weizmann
Institute of Science, Rehovot 76100, Israel and the ¶ Faculty of
Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
-amanitin, thus supporting transcription by RNA
polymerase II. We propose that snoRNA gene expression, similar to
protein-coding genes in this family, is regulated at the processing level.
*
This work was supported by a grant from the Israeli Ministry
of Health.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
972-3-531-8068; Fax: 972-3-535-1824; E-mail:
michaes@mail.biu.ac.il.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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