JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M010809200 on February 13, 2001

J. Biol. Chem., Vol. 276, Issue 18, 14884-14889, May 4, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/18/14884    most recent
M010809200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Galigniana, M. D.
Right arrow Articles by Pratt, W. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Galigniana, M. D.
Right arrow Articles by Pratt, W. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Evidence That the Peptidylprolyl Isomerase Domain of the hsp90-binding Immunophilin FKBP52 Is Involved in Both Dynein Interaction and Glucocorticoid Receptor Movement to the Nucleus*

Mario D. GalignianaDagger , Christine Radanyi§, Jack-Michel Renoir§, Paul R. Housley, and William B. PrattDagger ||

From the Dagger  Department of Pharmacology, The University of Michigan Medical School, Ann Arbor, Michigan 48109, the § Faculté de Pharmacie, UMR 8612 CNRS, Pharmacologie Cellulaire, 5 rue Jean-Baptiste Clément, Chatenay-Malabry Cedex, France, and the  Department of Pharmacology and Physiology, University of South Carolina School of Medicine, Columbia, South Carolina 29208

We have previously shown that immunoadsorption of the FKBP52 immunophilin component of steroid receptor·hsp90 heterocomplexes is accompanied by coadsorption of cytoplasmic dynein, a motor protein involved in retrograde transport of vesicles toward the nucleus. Coimmunoadsorption of dynein is competed by an expressed fragment of FKBP52 comprising its peptidylprolyl isomerase (PPIase) domain (Silverstein, A. M., Galigniana, M. D., Kanelakis, K. C., Radanyi, C., Renoir, J.-M., and Pratt, W. B. (1999) J. Biol. Chem. 52, 36980-36986). Here we show that cotransfection of 3T3 cells with the FKBP52 PPIase domain and a green fluorescent protein (GFP) glucocorticoid receptor (GR) chimera inhibits dexamethasone-dependent movement of the GFP-GR from the cytoplasm to the nucleus. Cotransfection with FKBP12 does not affect GFP-GR movement. Inhibition of movement by the FKBP52 PPIase domain is abrogated in cells treated with colcemid to eliminate microtubules prior to steroid addition. After withdrawal of colcemid, microtubules reform, and PPIase inhibition of GFP-GR movement is restored. These observations are consistent with the notion that FKBP52 targets retrograde movement of the GFP-GR along microtubules by linking the receptor to the dynein motor. Here, we also show that native GR·hsp90 heterocomplexes immunoadsorbed from L cell cytosol contain dynein and that GR·hsp90 heterocomplexes assembled in reticulocyte lysate contain cytoplasmic dynein in a manner that is competed by the PPIase domain of FKBP52.

* This work was supported by National Institutes of Health Grants CA28010 (to W.B.P.) and DK47951 (to P.R.H.), a grant from the Ligue Nationale contre le Cancer (Comités des Yvelines et du Cher), and Association pour la Recherche contre le Cancer Contract 9863 (to J.-M. R.). Cell Biology Core Laboratory services were supported in part by grant number NIH SP60 DK205972.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed: Dept. of Pharmacology, University of Michigan Medical School, MSRB III, Ann Arbor, MI 48109-0632. Tel.: 734-764-5414; Fax: 734-763-4450.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
H. D. McKeen, K. McAlpine, A. Valentine, D. J. Quinn, K. McClelland, C. Byrne, M. O'Rourke, S. Young, C. J. Scott, H. O. McCarthy, et al.
A Novel FK506-Like Binding Protein Interacts with the Glucocorticoid Receptor and Regulates Steroid Receptor Signaling
Endocrinology, November 1, 2008; 149(11): 5724 - 5734.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Becker, A. Sturm, J. E. Bron, K. Schirmer, and N. R. Bury
The A/B Domain of the Teleost Glucocorticoid Receptors Influences Partial Nuclear Localization in the Absence of Hormone
Endocrinology, September 1, 2008; 149(9): 4567 - 4576.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
X. Zhang, A. F. Clark, and T. Yorio
FK506-Binding Protein 51 Regulates Nuclear Transport of the Glucocorticoid Receptor {beta} and Glucocorticoid Responsiveness
Invest. Ophthalmol. Vis. Sci., March 1, 2008; 49(3): 1037 - 1047.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
B. Chambraud, H. Belabes, V. Fontaine-Lenoir, A. Fellous, and E. E. Baulieu
The immunophilin FKBP52 specifically binds to tubulin and prevents microtubule formation
FASEB J, September 1, 2007; 21(11): 2787 - 2797.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. E. Walker, R. Atanasiu, H. Lam, and A. Shrier
Co-chaperone FKBP38 Promotes HERG Trafficking
J. Biol. Chem., August 10, 2007; 282(32): 23509 - 23516.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
A. McMaster and D. W. Ray
Modelling the glucocorticoid receptor and producing therapeutic agents with anti-inflammatory effects but reduced side-effects
Exp Physiol, March 1, 2007; 92(2): 299 - 309.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Yong, Z. Yang, S. Periyasamy, H. Chen, S. Yucel, W. Li, L. Y. Lin, I. M. Wolf, M. J. Cohn, L. S. Baskin, et al.
Essential Role for Co-chaperone Fkbp52 but Not Fkbp51 in Androgen Receptor-mediated Signaling and Physiology
J. Biol. Chem., February 16, 2007; 282(7): 5026 - 5036.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
J. Hong, S. T. Kim, S. Tranguch, D. F Smith, and S. K Dey
Deficiency of co-chaperone immunophilin FKBP52 compromises sperm fertilizing capacity
Reproduction, February 1, 2007; 133(2): 395 - 403.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. M. Presman, E. Hoijman, N. R. Ceballos, M. D. Galigniana, and A. Pecci
Melatonin Inhibits Glucocorticoid Receptor Nuclear Translocation in Mouse Thymocytes
Endocrinology, November 1, 2006; 147(11): 5452 - 5459.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Smyczynski, F. Roudier, L. Gissot, E. Vaillant, O. Grandjean, H. Morin, T. Masson, Y. Bellec, D. Geelen, and J.-D. Faure
The C Terminus of the Immunophilin PASTICCINO1 Is Required for Plant Development and for Interaction with a NAC-like Transcription Factor
J. Biol. Chem., September 1, 2006; 281(35): 25475 - 25484.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
D. Gkika, C. N. Topala, J. G. J. Hoenderop, and R. J. M. Bindels
The immunophilin FKBP52 inhibits the activity of the epithelial Ca2+ channel TRPV5
Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1253 - F1259.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
X. Zhang, A. F. Clark, and T. Yorio
Heat Shock Protein 90 Is an Essential Molecular Chaperone for Nuclear Transport of Glucocorticoid Receptor {beta}
Invest. Ophthalmol. Vis. Sci., February 1, 2006; 47(2): 700 - 708.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Hinzpeter, J. Lipecka, F. Brouillard, M. Baudoin-Legros, M. Dadlez, A. Edelman, and J. Fritsch
Association between Hsp90 and the ClC-2 chloride channel upregulates channel function
Am J Physiol Cell Physiol, January 1, 2006; 290(1): C45 - C56.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
X. Zhang, A. F. Clark, and T. Yorio
Regulation of Glucocorticoid Responsiveness in Glaucomatous Trabecular Meshwork Cells by Glucocorticoid Receptor-{beta}
Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4607 - 4616.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. M. Wochnik, J. Ruegg, G. A. Abel, U. Schmidt, F. Holsboer, and T. Rein
FK506-binding Proteins 51 and 52 Differentially Regulate Dynein Interaction and Nuclear Translocation of the Glucocorticoid Receptor in Mammalian Cells
J. Biol. Chem., February 11, 2005; 280(6): 4609 - 4616.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. D. Galigniana, Y. Morishima, P. A. Gallay, and W. B. Pratt
Cyclophilin-A Is Bound through Its Peptidylprolyl Isomerase Domain to the Cytoplasmic Dynein Motor Protein Complex
J. Biol. Chem., December 31, 2004; 279(53): 55754 - 55759.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. M. Harrell, P. J. M. Murphy, Y. Morishima, H. Chen, J. F. Mansfield, M. D. Galigniana, and W. B. Pratt
Evidence for Glucocorticoid Receptor Transport on Microtubules by Dynein
J. Biol. Chem., December 24, 2004; 279(52): 54647 - 54654.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. J. M. Murphy, M. D. Galigniana, Y. Morishima, J. M. Harrell, R. P. S. Kwok, M. Ljungman, and W. B. Pratt
Pifithrin-{alpha} Inhibits p53 Signaling after Interaction of the Tumor Suppressor Protein with hsp90 and Its Nuclear Translocation
J. Biol. Chem., July 16, 2004; 279(29): 30195 - 30201.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Sanokawa-Akakura, H. Dai, S. Akakura, D. Weinstein, J. E. Fajardo, S. E. Lang, S. Wadsworth, J. Siekierka, and R. B. Birge
A Novel Role for the Immunophilin FKBP52 in Copper Transport
J. Biol. Chem., July 2, 2004; 279(27): 27845 - 27848.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
L. Yan, R. L. Cerny, and J. D. Cirillo
Evidence that hsp90 Is Involved in the Altered Interactions of Acanthamoeba castellanii Variants with Bacteria
Eukaryot. Cell, June 1, 2004; 3(3): 567 - 578.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Wu, P. Li, Y. Liu, Z. Lou, Y. Ding, C. Shu, S. Ye, M. Bartlam, B. Shen, and Z. Rao
3D structure of human FK506-binding protein 52: Implications for the assembly of the glucocorticoid receptor/Hsp90/immunophilin heterocomplex
PNAS, June 1, 2004; 101(22): 8348 - 8353.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. D. Galigniana, J. M. Harrell, H. M. O'Hagen, M. Ljungman, and W. B. Pratt
Hsp90-binding Immunophilins Link p53 to Dynein During p53 Transport to the Nucleus
J. Biol. Chem., May 21, 2004; 279(21): 22483 - 22489.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
N. Z. Gerges, I. C. Tran, D. S. Backos, J. M. Harrell, M. Chinkers, W. B. Pratt, and J. A. Esteban
Independent Functions of hsp90 in Neurotransmitter Release and in the Continuous Synaptic Cycling of AMPA Receptors
J. Neurosci., May 19, 2004; 24(20): 4758 - 4766.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. D. Freedman and K. R. Yamamoto
Importin 7 and Importin {alpha}/Importin {beta} Are Nuclear Import Receptors for the Glucocorticoid Receptor
Mol. Biol. Cell, May 1, 2004; 15(5): 2276 - 2286.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Zhong, K. Qing, Y. Si, L. Chen, M. Tan, and A. Srivastava
Heat-shock Treatment-mediated Increase in Transduction by Recombinant Adeno-associated Virus 2 Vectors Is Independent of the Cellular Heat-shock Protein 90
J. Biol. Chem., March 26, 2004; 279(13): 12714 - 12723.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. Gonzalez-Alegre and H. L. Paulson
Aberrant Cellular Behavior of Mutant TorsinA Implicates Nuclear Envelope Dysfunction in DYT1 Dystonia
J. Neurosci., March 17, 2004; 24(11): 2593 - 2601.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Thomas, N. Dadgar, A. Aphale, J. M. Harrell, R. Kunkel, W. B. Pratt, and A. P. Lieberman
Androgen Receptor Acetylation Site Mutations Cause Trafficking Defects, Misfolding, and Aggregation Similar to Expanded Glutamine Tracts
J. Biol. Chem., February 27, 2004; 279(9): 8389 - 8395.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. M. Perez-Perez, M. R. Ponce, and J. L. Micol
The ULTRACURVATA2 Gene of Arabidopsis Encodes an FK506-Binding Protein Involved in Auxin and Brassinosteroid Signaling
Plant Physiology, January 1, 2004; 134(1): 101 - 117.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Kunz, R. Sandoval, P. Carlsson, J. Carlstedt-Duke, J. W. Bloom, and R. L. Miesfeld
Identification of a Novel Glucocorticoid Receptor Mutation in Budesonide-Resistant Human Bronchial Epithelial Cells
Mol. Endocrinol., December 1, 2003; 17(12): 2566 - 2582.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. S. Dwivedi, A. Breiman, and E. M. Herman
Differential distribution of the cognate and heat-stress-induced isoforms of high Mr cis-trans prolyl peptidyl isomerase (FKBP) in the cytoplasm and nucleoplasm
J. Exp. Bot., December 1, 2003; 54(393): 2679 - 2689.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. M. Verrills, B. J. Walsh, G. S. Cobon, P. G. Hains, and M. Kavallaris
Proteome Analysis of Vinca Alkaloid Response and Resistance in Acute Lymphoblastic Leukemia Reveals Novel Cytoskeletal Alterations
J. Biol. Chem., November 14, 2003; 278(46): 45082 - 45093.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Lees, D. J. Peet, and M. L. Whitelaw
Defining the Role for XAP2 in Stabilization of the Dioxin Receptor
J. Biol. Chem., September 19, 2003; 278(38): 35878 - 35888.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F. Mascia, V. Mariani, G. Girolomoni, and S. Pastore
Blockade of the EGF Receptor Induces a Deranged Chemokine Expression in Keratinocytes Leading to Enhanced Skin Inflammation
Am. J. Pathol., July 1, 2003; 163(1): 303 - 312.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. R. Hubler, W. B. Denny, D. L. Valentine, J. Cheung-Flynn, D. F. Smith, and J. G. Scammell
The FK506-Binding Immunophilin FKBP51 Is Transcriptionally Regulated by Progestin and Attenuates Progestin Responsiveness
Endocrinology, June 1, 2003; 144(6): 2380 - 2387.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
W. B. Pratt and D. O. Toft
Regulation of Signaling Protein Function and Trafficking by the hsp90/hsp70-Based Chaperone Machinery
Experimental Biology and Medicine, February 1, 2003; 228(2): 111 - 133.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. S. Edinger, S. C. Watkins, D. Pearce, and J. P. Johnson
Effect of immunosuppressive agents on glucocorticoid receptor function in A6 cells
Am J Physiol Renal Physiol, August 1, 2002; 283(2): F254 - F261.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Piwien-Pilipuk, A. Ayala, A. Machado, and M. D. Galigniana
Impairment of Mineralocorticoid Receptor (MR)-dependent Biological Response by Oxidative Stress and Aging. CORRELATION WITH POST-TRANSLATIONAL MODIFICATION OF MR AND DECREASED ADP-RIBOSYLATABLE LEVEL OF ELONGATION FACTOR 2 IN KIDNEY CELLS
J. Biol. Chem., March 29, 2002; 277(14): 11896 - 11903.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.