JBC Advanced Peptides, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M007958200 on February 13, 2001

J. Biol. Chem., Vol. 276, Issue 18, 15082-15089, May 4, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/18/15082    most recent
M007958200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tanaka, M.
Right arrow Articles by Komatsu, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tanaka, M.
Right arrow Articles by Komatsu, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Forkhead Family Transcription Factor FKHRL1 Is Expressed in Human Megakaryocytes
REGULATION OF CELL CYCLING AS A DOWNSTREAM MOLECULE OF THROMBOPOIETIN SIGNALING*

Masaru TanakaDagger , Keita KiritoDagger , Yoshifumi KashiiDagger §, Mie UchidaDagger , Tomoko WatanabeDagger , Hitoshi Endo, Tomoyuki Endoh||, Ken-ichi Sawada||, Keiya OzawaDagger , and Norio KomatsuDagger **

From the Departments of Dagger  Hematology, § Pediatrics, and  Biochemistry, Jichi Medical School, Tochigi, 329-0498 Japan and the || Department of Internal Medicine II, Hokkaido University School of Medicine, Sapporo, Japan

FKHRL1, a member of the Forkhead transcription factor family, is one of the downstream molecules of phosphatidylinositol 3-kinase-Akt. This molecule is a mammalian homolog of DAF-16, which plays an important role in the longevity of Caenorhabditis elegans. In this study we found that Akt and FKHRL1 proteins were detectable in highly purified normal human megakaryocytes and that these molecules were actually phosphorylated by thrombopoietin (TPO). To clarify the functional role of FKHRL1 in TPO signaling, we established a tetracycline-inducible system in the human TPO-dependent leukemia cell line UT-7/TPO. Induced expression of active FKHRL1 led to cell cycle arrest at G0/G1 phase in this cell line. These results suggest that FKHRL1 plays an important role in the cell cycle of megakaryocytic cells as one of the downstream target molecules of phosphatidylinositol 3-kinase-Akt, presumably mediated through the activation or inactivation of cell cycle-associated gene(s).

* This work was supported by grants-in-aid for cancer research and scientific research from the Ministry of Education, Science and Culture of Japan.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

** To whom correspondence should be addressed: Dept. of Hematology, Jichi Medical School Minamikawachi-machi, Kawachi-gun, Tochigi-ken 329-0498, Japan. Tel.: 81-285-58-7353; Fax: 81-285-44-5258; E-mail: nkomatsu@ms.jichi.ac.jp.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Physiol. GenomicsHome page
P. G. Fuhrken, C. Chen, P. A. Apostolidis, M. Wang, W. M. Miller, and E. T. Papoutsakis
Gene Ontology-driven transcriptional analysis of CD34+ cell-initiated megakaryocytic cultures identifies new transcriptional regulators of megakaryopoiesis
Physiol Genomics, April 1, 2008; 33(2): 159 - 169.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
A. L. Drayer, S. G. M. Olthof, and E. Vellenga
Mammalian Target of Rapamycin Is Required for Thrombopoietin-Induced Proliferation of Megakaryocyte Progenitors
Stem Cells, January 1, 2006; 24(1): 105 - 114.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Daitoku, M. Hatta, H. Matsuzaki, S. Aratani, T. Ohshima, M. Miyagishi, T. Nakajima, and A. Fukamizu
Silent information regulator 2 potentiates Foxo1-mediated transcription through its deacetylase activity
PNAS, July 6, 2004; 101(27): 10042 - 10047.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. A. Cunningham, Q. Zhu, and J. M. Hammond
FoxO1a Can Alter Cell Cycle Progression by Regulating the Nuclear Localization of p27kip in Granulosa Cells
Mol. Endocrinol., July 1, 2004; 18(7): 1756 - 1767.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
M. Uchida, T. Watanabe, M. Kunitama, M. Mori, S. Kikuchi, K. Yoshida, K. Kirito, T. Nagai, K. Ozawa, and N. Komatsu
Erythropoietin Overcomes Imatinib-Induced Apoptosis and Induces Erythroid Differentiation in TF-1/bcr-abl Cells
Stem Cells, July 1, 2004; 22(4): 609 - 616.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
W.-C. Tsai, N. Bhattacharyya, L.-Y. Han, J. A. Hanover, and M. M. Rechler
Insulin Inhibition of Transcription Stimulated by the Forkhead Protein Foxo1 Is Not Solely due to Nuclear Exclusion
Endocrinology, December 1, 2003; 144(12): 5615 - 5622.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Perrot and M. M. Rechler
Characterization of Insulin Inhibition of Transactivation by a C-terminal Fragment of the Forkhead Transcription Factor Foxo1 in Rat Hepatoma Cells
J. Biol. Chem., July 3, 2003; 278(28): 26111 - 26119.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
H. Tran, A. Brunet, E. C. Griffith, and M. E. Greenberg
The Many Forks in FOXO's Road
Sci. Signal., March 4, 2003; 2003(172): re5 - re5.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Komatsu, T. Watanabe, M. Uchida, M. Mori, K. Kirito, S. Kikuchi, Q. Liu, T. Tauchi, K. Miyazawa, H. Endo, et al.
A Member of Forkhead Transcription Factor FKHRL1 Is a Downstream Effector of STI571-induced Cell Cycle Arrest in BCR-ABL-expressing Cells
J. Biol. Chem., February 14, 2003; 278(8): 6411 - 6419.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y.-W. Qiang, E. Kopantzev, and S. Rudikoff
Insulinlike growth factor-I signaling in multiple myeloma: downstream elements, functional correlates, and pathway cross-talk
Blood, May 13, 2002; 99(11): 4138 - 4146.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. S. Richards, S. C. Sharma, A. E. Falender, and Y. H. Lo
Expression of FKHR, FKHRL1, and AFX Genes in the Rodent Ovary: Evidence for Regulation by IGF-I, Estrogen, and the Gonadotropins
Mol. Endocrinol., March 1, 2002; 16(3): 580 - 599.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Kirito, T. Watanabe, K.-i. Sawada, H. Endo, K. Ozawa, and N. Komatsu
Thrombopoietin Regulates Bcl-xL Gene Expression through Stat5 and Phosphatidylinositol 3-Kinase Activation Pathways
J. Biol. Chem., March 1, 2002; 277(10): 8329 - 8337.
[Abstract] [Full Text] [PDF]

Home page
 Recent Prog Horm ResHome page
J. S. Richards, D. L. Russell, S. Ochsner, M. Hsieh, K. H. Doyle, A. E. Falender, Y. K. Lo, and S. C. Sharma
Novel Signaling Pathways That Control Ovarian Follicular Development, Ovulation, and Luteinization
Recent Prog. Horm. Res., January 1, 2002; 57(1): 195 - 220.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
P. C. Hollenhorst, G. Pietz, and C. A. Fox
Mechanisms controlling differential promoter-occupancy by the yeast forkhead proteins Fkh1p and Fkh2p: implications for regulating the cell cycle and differentiation
Genes & Dev., September 15, 2001; 15(18): 2445 - 2456.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.