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J. Biol. Chem., Vol. 276, Issue 18, 15453-15457, May 4, 2001
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From the Exposure of Semliki Forest virus 1 to mildly
acidic conditions results in conformational changes of the viral spike
proteins, which in turn leads to a pore formation across its membrane.
The ability to form a pore has been ascribed to the ectodomain of the
Semliki Forest virus (SFV) E1 spike protein. To elucidate whether the
E1 protein per se is sufficient for low
pH-dependent pore formation, we expressed E1 in
Escherichia coli in an inducible manner using the pET11c
expression system. The data obtained clearly showed that the E1 protein
was expressed in the bacterial cell membrane and that exposure of
E. coli expressing the SFV E1 protein to low pH (<6.2)
resulted in a permeability change of the membrane. Thus, we conclude
that the E1 protein of SFV per se is sufficient to promote
pore formation under mildly acidic conditions.
Expression of Semliki Forest Virus E1 Protein in
Escherichia coli
LOW pH-INDUCED PORE FORMATION*
,§, and¶
Department of Chemistry and Biochemistry,
University of Bern, Freiestrasse 3, 3012 Bern, Switzerland and
¶ ZLB Bioplasma AG, Wankdorfstrasse 10, 3000 Bern
22, Switzerland
*
This work was supported in part by Swiss National Science
Foundation Grant 31-49217.96 (to C. K.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of
Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland. Tel.: 41-31-6314339; Fax: 41-31-6314887;
E-mail: kempf@ibc.unibe.ch.
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