JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M100406200 on February 2, 2001

J. Biol. Chem., Vol. 276, Issue 19, 15728-15735, May 11, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/19/15728    most recent
M100406200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Feng, Z.-P.
Right arrow Articles by Zamponi, G. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Feng, Z.-P.
Right arrow Articles by Zamponi, G. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Residue Gly1326 of the N-type Calcium Channel alpha 1B Subunit Controls Reversibility of omega -Conotoxin GVIA and MVIIA Block*

Zhong-Ping FengDagger §||, Jawed HamidDagger , Clinton DoeringDagger §, Gregory M. Bosey**, Terrance P. Snutch**Dagger Dagger , and Gerald W. ZamponiDagger §§

From the Dagger  Departments of Physiology & Biophysics and Pharmacology & Therapeutics, Neuroscience Research Group, University of Calgary, Calgary, Alberta T2N 4N1, Canada, § NeuroMed Technologies Inc., Vancouver V6T 1Z4, Canada, and the ** Biotechnology Laboratory, University of British Columbia, Vancouver V6T 1Z3, Canada

We recently reported that amino acid residues contained within a putative EF hand motif in the domain III S5-H5 region of the alpha 1B subunit affected the relative barium:calcium permeability of N-type calcium channels (Feng, Z. P., Hamid, J., Doering, C., Jarvis, S. E., Bosey, G. M., Bourinet, E., Snutch, T. P., and Zamponi, G. W. (2001) J. Biol. Chem. 276, 5726-5730). Since this region partially overlaps with residues previously implicated in block of the channel by omega -conotoxin GVIA, we assessed the effects of mutations in the putative EF hand domain on channel block by omega -conotoxin GVIA and the structurally related omega -conotoxin MVIIA. Both of the toxins irreversibly block the activity of wild type alpha 1B N-type channels. We find that in addition to previously identified amino acid residues, residues in positions 1326 and 1332 are important determinants of omega -conotoxin GVIA blockade. Substitution of residue Glu1332 to arginine slows the time course of development of block. Point mutations in position Gly1326 to either arginine, glutamic acid, or proline dramatically decrease the time constant for development of the block. Additionally, in the G1326P mutant channel activity was almost completely recovered following washout. A qualitatively similar result was obtained with omega -conotoxin MVIIA, suggesting that common molecular determinants underlie block by these two toxins. Taken together the data suggest that residue Gly1326 may form a barrier, which controls the access of peptide toxins to their blocking site within the outer vestibule of the channel pore and also stabilizes the toxin-channel interaction.

* This work was supported by operating grants (to G. W. Z.) from the Canadian Institutes of Health Research (CIHR) and the Heart and Stroke Foundation of Alberta and the Northwest Territories and through a Scholarship Award (to G. W. Z.) from the EJLB Foundation.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

These authors contributed equally to this study.

|| Recipient of a postdoctoral fellowship from the Natural Science and Engineering Research Council of Canada.

Dagger Dagger Holds CIHR operating funds and a Senior Scientist award from the CIHR.

§§ Holds Faculty Scholarships from the Alberta Heritage Foundation for Medical Research (AHFMR) and the CIHR and is the Novartis Investigator in Schizophrenia Research. To whom correspondence should be addressed: Dept. of Physiology and Biophysics, University of Calgary, 3330 Hospital Dr. NW, Calgary, Alberta T2N 4N1, Canada. Tel.: 403-220-8687; Fax: 403-210-8106; E-mail: zamponi@ucalgary.ca.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. J. Liao, P. Safa, Y.-R. Chen, R. A. Sobel, E. S. Boyden, and R. W. Tsien
Anti-Ca2+ channel antibody attenuates Ca2+ currents and mimics cerebellar ataxia in vivo
PNAS, February 19, 2008; 105(7): 2705 - 2710.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
H.-S. Sun, K. Hui, D. W. K. Lee, and Z.-P. Feng
Zn2+ Sensitivity of High- and Low-Voltage Activated Calcium Channels
Biophys. J., August 15, 2007; 93(4): 1175 - 1183.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Liu, J. Dai, L. Dai, M. Deng, Z. Hu, W. Hu, and S. Liang
Function and Solution Structure of Huwentoxin-X, a Specific Blocker of N-type Calcium Channels, from the Chinese Bird Spider Ornithoctonus huwena
J. Biol. Chem., March 31, 2006; 281(13): 8628 - 8635.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. Li, D. Lee, L. Wang, M. Khvotchev, S. K. Chiew, L. Arunachalam, T. Collins, Z.-P. Feng, and S. Sugita
N-Terminal Insertion and C-Terminal Ankyrin-Like Repeats of {alpha}-Latrotoxin Are Critical for Ca2+-Dependent Exocytosis
J. Neurosci., November 2, 2005; 25(44): 10188 - 10197.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Mould, T. Yasuda, C. I. Schroeder, A. M. Beedle, C. J. Doering, G. W. Zamponi, D. J. Adams, and R. J. Lewis
The {alpha}2{delta} Auxiliary Subunit Reduces Affinity of {omega}-Conotoxins for Recombinant N-type (Cav2.2) Calcium Channels
J. Biol. Chem., August 13, 2004; 279(33): 34705 - 34714.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
R. L. Brown, L. L. Lynch, T. L. Haley, and R. Arsanjani
Pseudechetoxin Binds to the Pore Turret of Cyclic Nucleotide-gated Ion Channels
J. Gen. Physiol., November 24, 2003; 122(6): 749 - 760.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. D. Spafford, L. Chen, Z.-P. Feng, A. B. Smit, and G. W. Zamponi
Expression and Modulation of an Invertebrate Presynaptic Calcium Channel {alpha}1 Subunit Homolog
J. Biol. Chem., June 6, 2003; 278(23): 21178 - 21187.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z.-P. Feng, C. J. Doering, R. J. Winkfein, A. M. Beedle, J. D. Spafford, and G. W. Zamponi
Determinants of Inhibition of Transiently Expressed Voltage-gated Calcium Channels by {omega}-Conotoxins GVIA and MVIIA
J. Biol. Chem., May 23, 2003; 278(22): 20171 - 20178.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. J. Adams, A. B. Smith, C. I. Schroeder, T. Yasuda, and R. J. Lewis
omega -Conotoxin CVID Inhibits a Pharmacologically Distinct Voltage-sensitive Calcium Channel Associated with Transmitter Release from Preganglionic Nerve Terminals
J. Biol. Chem., January 31, 2003; 278(6): 4057 - 4062.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Liang and K. S. Elmslie
Rapid and Reversible Block of N-Type Calcium Channels (CaV 2.2) by omega -Conotoxin GVIA in the Absence of Divalent Cations
J. Neurosci., October 15, 2002; 22(20): 8884 - 8890.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
T. R. Cummins, F. Aglieco, and S. D. Dib-Hajj
Critical Molecular Determinants of Voltage-Gated Sodium Channel Sensitivity to {micro}-Conotoxins GIIIA/B
Mol. Pharmacol., May 1, 2002; 61(5): 1192 - 1201.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Kaneko, C. B. Cooper, N. Nishioka, H. Yamasaki, A. Suzuki, S. E. Jarvis, A. Akaike, M. Satoh, and G. W. Zamponi
Identification and Characterization of Novel Human Cav2.2 (alpha 1B) Calcium Channel Variants Lacking the Synaptic Protein Interaction Site
J. Neurosci., January 1, 2002; 22(1): 82 - 92.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.