JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M011408200 on February 14, 2001

J. Biol. Chem., Vol. 276, Issue 19, 16083-16091, May 11, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/19/16083    most recent
M011408200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Southall, M. D.
Right arrow Articles by Vasko, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Southall, M. D.
Right arrow Articles by Vasko, M. R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Prostaglandin Receptor Subtypes, EP3C and EP4, Mediate the Prostaglandin E2-induced cAMP Production and Sensitization of Sensory Neurons*

M. D. SouthallDagger and M. R. VaskoDagger §

From the Dagger  Department of Pharmacology and Toxicology and  Department of Anesthesia, School of Medicine, Indiana University, Indianapolis, Indiana 46202-5126

Although a number of prostaglandin E2 (PGE2) receptor subtypes have been cloned, limited studies have been performed to elucidate subtypes that subserve specific actions of this eicosanoid, in part because of a paucity of selective receptor antagonists. Using reverse transcription-polymerase chain reaction (PCR) and antisense oligonucleotides, we examined which prostaglandin E2 receptor (EP receptor) subtypes are expressed in sensory neurons and which mediate the PGE2-induced increase in cAMP production and augmentation of peptide release. Reverse transcription-PCR of cDNA isolated from rat sensory neurons grown in culture revealed PCR products for the EP1, EP2, EP3C, and EP4 receptor subtypes but not the EP3A or EP3B. Preexposing neuronal cultures for 48 h to antisense oligonucleotides of EP3C and EP4 mRNA diminished expression of the respective receptors by ~80%, abolished the PGE2-stimulated production of cAMP, and blocked the ability of PGE2 to augment release of immunoreactive substance P and calcitonin gene-related peptide. Pretreating with individual antisense against the EP2, EP3C, or EP4 receptors or combinations of missense oligonucleotides had no effect on PGE2-induced activity. Treatment with antisense to EP3C and EP4 receptor subtypes did not alter the ability of forskolin to increase cAMP or enhance peptide release. These results demonstrate that sensory neurons are capable of expressing multiple EP receptor subtypes but that only the EP3C and EP4 receptors mediate PGE2-induced sensitization of sensory neurons.

* This work was supported in part by National Institutes of Health Grant RO1-NS34159 (to M. R. V.) and by a fellowship from the American Heart Association, Indiana Affiliate (to M. D. S.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ To whom correspondence should be addressed: Dept. of Pharmacology & Toxicology, 635 Barnhill Dr., School of Medicine, Indiana University, Indianapolis, IN 46202-5126. Tel.: 317-274-1575; Fax: 317-274-1560; E-mail: vaskom@iupui.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
S. Jain, G. Chakraborty, R. Raja, S. Kale, and G. C. Kundu
Prostaglandin E2 Regulates Tumor Angiogenesis in Prostate Cancer
Cancer Res., October 1, 2008; 68(19): 7750 - 7759.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Schnizler, L. P. Shutov, M. J. Van Kanegan, M. A. Merrill, B. Nichols, G. S. McKnight, S. Strack, J. W. Hell, and Y. M. Usachev
Protein Kinase A Anchoring via AKAP150 Is Essential for TRPV1 Modulation by Forskolin and Prostaglandin E2 in Mouse Sensory Neurons
J. Neurosci., May 7, 2008; 28(19): 4904 - 4917.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
A.M. Patwardhan, J. Vela, J. Farugia, K. Vela, and K.M. Hargreaves
Trigeminal Nociceptors Express Prostaglandin Receptors
J. Dent. Res., March 1, 2008; 87(3): 262 - 266.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. Wang, Y. Gu, G.-W. Li, and L.-Y. M. Huang
A critical role of the cAMP sensor Epac in switching protein kinase signalling in prostaglandin E2-induced potentiation of P2X3 receptor currents in inflamed rats
J. Physiol., October 1, 2007; 584(1): 191 - 203.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. Meisdalen, O. F. Dajani, T. Christoffersen, and D. Sandnes
Prostaglandins Enhance Epidermal Growth Factor-Induced DNA Synthesis in Hepatocytes by Stimulation of E Prostanoid 3 and F Prostanoid Receptors
J. Pharmacol. Exp. Ther., September 1, 2007; 322(3): 1044 - 1050.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. Nakao, A. Murase, H. Ohshiro, T. Okumura, K. Taniguchi, Y. Murata, M. Masuda, T. Kato, Y. Okumura, and J. Takada
CJ-023,423, a Novel, Potent and Selective Prostaglandin EP4 Receptor Antagonist with Antihyperalgesic Properties
J. Pharmacol. Exp. Ther., August 1, 2007; 322(2): 686 - 694.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
A. Dogrul, S. E. Gulmez, M. S. Deveci, H. Gul, M. H. Ossipov, F. Porreca, and F. C. Tulunay
The Local Antinociceptive Actions of Nonsteroidal Antiinflammatory Drugs in the Mouse Radiant Heat Tail-Flick Test
Anesth. Analg., April 1, 2007; 104(4): 927 - 935.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Han, J. D. Ritzenthaler, B. Wingerd, H. N. Rivera, and J. Roman
Extracellular Matrix Fibronectin Increases Prostaglandin E2 Receptor Subtype EP4 in Lung Carcinoma Cells through Multiple Signaling Pathways: THE ROLE OF AP-2
J. Biol. Chem., March 16, 2007; 282(11): 7961 - 7972.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
C.-R. Lin, F. Amaya, L. Barrett, H. Wang, J. Takada, T. A. Samad, and C. J. Woolf
Prostaglandin E2 Receptor EP4 Contributes to Inflammatory Pain Hypersensitivity
J. Pharmacol. Exp. Ther., December 1, 2006; 319(3): 1096 - 1103.
[Abstract] [Full Text] [PDF]

Home page
 Molecular Cancer TherapeuticsHome page
T. Payner, H. A. Leaver, B. Knapp, I. R. Whittle, O. C. Trifan, S. Miller, and M. T. Rizzo
Microsomal prostaglandin E synthase-1 regulates human glioma cell growth via prostaglandin E2-dependent activation of type II protein kinase A.
Mol. Cancer Ther., July 1, 2006; 5(7): 1817 - 1826.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
T. Schmitz, B. A Levine, and P. W Nathanielsz
Localization and steroid regulation of prostaglandin E2 receptor protein expression in ovine cervix.
Reproduction, April 1, 2006; 131(4): 743 - 750.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
X.-J. Song, Z.-B. Wang, Q. Gan, and E. T. Walters
cAMP and cGMP Contribute to Sensory Neuron Hyperexcitability and Hyperalgesia in Rats With Dorsal Root Ganglia Compression
J Neurophysiol, January 1, 2006; 95(1): 479 - 492.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Han, J. D. Ritzenthaler, B. Wingerd, and J. Roman
Activation of Peroxisome Proliferator-activated Receptor {beta}/{delta} (PPAR{beta}/{delta}) Increases the Expression of Prostaglandin E2 Receptor Subtype EP4: THE ROLES OF PHOSPHATIDYLINOSITOL 3-KINASE AND CCAAT/ENHANCER-BINDING PROTEIN {beta}
J. Biol. Chem., September 30, 2005; 280(39): 33240 - 33249.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C.-H. Tang, R.-S. Yang, and W.-M. Fu
Prostaglandin E2 Stimulates Fibronectin Expression through EP1 Receptor, Phospholipase C, Protein Kinase C{alpha}, and c-Src Pathway in Primary Cultured Rat Osteoblasts
J. Biol. Chem., June 17, 2005; 280(24): 22907 - 22916.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
U. C. Kopp, M. Z. Cicha, K. Nakamura, R. M. Nusing, L. A. Smith, and T. Hokfelt
Activation of EP4 receptors contributes to prostaglandin E2-mediated stimulation of renal sensory nerves
Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1269 - F1282.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Cheng, H. Jia, M. Lohr, A. Bagherzadeh, D. I. R. Holmes, D. Selwood, and I. Zachary
Anti-chemorepulsive Effects of Vascular Endothelial Growth Factor and Placental Growth Factor-2 in Dorsal Root Ganglion Neurons Are Mediated via Neuropilin-1 and Cyclooxygenase-derived Prostanoid Production
J. Biol. Chem., July 16, 2004; 279(29): 30654 - 30661.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. R. Ghilardi, C. I. Svensson, S. D. Rogers, T. L. Yaksh, and P. W. Mantyh
Constitutive Spinal Cyclooxygenase-2 Participates in the Initiation of Tissue Injury-Induced Hyperalgesia
J. Neurosci., March 17, 2004; 24(11): 2727 - 2732.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K.-J. Bar, G. Natura, A. Telleria-Diaz, P. Teschner, R. Vogel, E. Vasquez, H.-G. Schaible, and A. Ebersberger
Changes in the Effect of Spinal Prostaglandin E2 during Inflammation: Prostaglandin E (EP1-EP4) Receptors in Spinal Nociceptive Processing of Input from the Normal or Inflamed Knee Joint
J. Neurosci., January 21, 2004; 24(3): 642 - 651.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. P. Mohapatra and C. Nau
Desensitization of Capsaicin-activated Currents in the Vanilloid Receptor TRPV1 Is Decreased by the Cyclic AMP-dependent Protein Kinase Pathway
J. Biol. Chem., December 12, 2003; 278(50): 50080 - 50090.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. Durand, B. Fromy, M. Tartas, A. Jardel, J. L. Saumet, and P. Abraham
Prolonged aspirin inhibition of anodal vasodilation is not due to the trafficking delay of neural mediators
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2003; 285(1): R155 - R161.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Hatae, A. Kita, S. Tanaka, Y. Sugimoto, and A. Ichikawa
Induction of Adherent Activity in Mastocytoma P-815 Cells by the Cooperation of Two Prostaglandin E2 Receptor Subtypes, EP3 and EP4
J. Biol. Chem., May 9, 2003; 278(20): 17977 - 17981.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
C. Distler, P. K. Rathee, K. S. Lips, O. Obreja, W. Neuhuber, and M. Kress
Fast Ca2+-Induced Potentiation of Heat-Activated Ionic Currents Requires cAMP/PKA Signaling and Functional AKAP Anchoring
J Neurophysiol, May 1, 2003; 89(5): 2499 - 2505.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Walch, E. Clavarino, and P. L. Morris
Prostaglandin (PG) FP and EP1 Receptors Mediate PGF2{alpha} and PGE2 Regulation of Interleukin-1{beta} Expression in Leydig Cell Progenitors
Endocrinology, April 1, 2003; 144(4): 1284 - 1291.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Q. Gu, K. Kwong, and L.-Y. Lee
Ca2+ Transient Evoked by Chemical Stimulation Is Enhanced by PGE2 in Vagal Sensory Neurons: Role of cAMP/PKA Signaling Pathway
J Neurophysiol, April 1, 2003; 89(4): 1985 - 1993.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. D. Southall, T. Li, L. S. Gharibova, Y. Pei, G. D. Nicol, and J. B. Travers
Activation of Epidermal Vanilloid Receptor-1 Induces Release of Proinflammatory Mediators in Human Keratinocytes
J. Pharmacol. Exp. Ther., January 1, 2003; 304(1): 217 - 222.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Dohadwala, R. K. Batra, J. Luo, Y. Lin, K. Krysan, M. Pold, S. Sharma, and S. M. Dubinett
Autocrine/Paracrine Prostaglandin E2 Production by Non-small Cell Lung Cancer Cells Regulates Matrix Metalloproteinase-2 and CD44 in Cyclooxygenase-2-dependent Invasion
J. Biol. Chem., December 20, 2002; 277(52): 50828 - 50833.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Kwong and L.-Y. Lee
PGE2 sensitizes cultured pulmonary vagal sensory neurons to chemical and electrical stimuli
J Appl Physiol, October 1, 2002; 93(4): 1419 - 1428.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
J. D. Richardson and M. R. Vasko
Cellular Mechanisms of Neurogenic Inflammation
J. Pharmacol. Exp. Ther., September 1, 2002; 302(3): 839 - 845.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. K. Rathee, C. Distler, O. Obreja, W. Neuhuber, G. K. Wang, S.-Y. Wang, C. Nau, and M. Kress
PKA/AKAP/VR-1 Module: A Common Link of Gs-Mediated Signaling to Thermal Hyperalgesia
J. Neurosci., June 1, 2002; 22(11): 4740 - 4745.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. L. Borgland, M. Connor, R. M. Ryan, H. J. Ball, and M. J. Christie
Prostaglandin E2 inhibits calcium current in two sub-populations of acutely isolated mouse trigeminal sensory neurons
J. Physiol., March 1, 2002; 539(2): 433 - 444.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.