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J. Biol. Chem., Vol. 276, Issue 19, 16100-16106, May 11, 2001
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From the The CD15 carbohydrate epitope is expressed in
mature human neutrophils, monocytes, and promyelocytes. We aimed to
determine the
CD15 Expression in Mature Granulocytes Is Determined by
1,3-Fucosyltransferase IX, but in Promyelocytes and Monocytes by
1,3-Fucosyltransferase IV*
,,,,,§,
,§§
Division of Cell Biology, Institute of Life
Science, Soka University, Tokyo 192-8577, the
Laboratory of Gene Function Analysis,
Institute of Molecular and Cell Biology, National Institute of Advanced
Industrial Science and Technology, Central-2, 1-1-1 Umezono, OSL-C2,
Tsuluba, Ibaraki 305-8568, the § Department of Forensic
Medicine, Yamagata University School of Medicine, Yamagata 990-9585, the ¶ Division of Hemopoiesis, Institute of Hematology, Jichi
Medical School, Tochigi 329-0498, the Division of Obstetrics,
Fussa Hospital, Tokyo 197-0012, and ** Tokyo Research Laboratory, Kyowa
Hakko Kogyo Co., Ltd., Tokyo 194-8533, Japan
1,3-fucosyltransferase responsible for the expression
of CD15 in each subpopulation of leukocytes. Three
1,3-fucosyltransferases, FUT4, FUT7, and FUT9, are expressed in
human leukocytes. We demonstrated that FUT9 exhibits 20-fold stronger
activity for CD15 synthesis than FUT4, whereas FUT4 exhibits 4.5-fold
stronger activity for CDw65 synthesis than FUT9. By competitive reverse
transcriptase-polymerase chain reaction, FUT9 was found to be strongly
expressed in mature granulocytes and peripheral blood mononuclear cell,
but not in monocytes. CD34+ and CD15+
cells in cord blood and myeloid cell lines (HL-60 and U937) did not
express FUT9 at all. FUT4 transcripts were ubiquitously expressed in
all blood cells and all cultured cell lines, with HL-60 and U937 cells
in particular expressing a number of FUT4 transcripts. Transfection of
the FUT9 gene into Jurkat and U937 cells demonstrated that
FUT9 has the potential to express CD15 in myeloid and lymphoid cells.
These findings suggest that the expression of CD15 in mature granulocytes is directed by FUT9, whereas it is determined in promyelocytes and monocytes by FUT4. Measurement of CD15 synthesizing activity in cell homogenates of each cell population using the polylactosamine acceptor further supported these conclusions.
*
This work was supported in part by Grant-in-Aid for
Scientific Research on Priority Areas 10178104 (to H. N.) from the
Ministry of Education, Science, and Culture of Japan.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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