|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 19, 16216-16222, May 11, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the The Saccharomyces cerevisiae ALR1
(YOL130w) gene product Alr1p is the first known candidate for a
Mg2+ transport system in eukaryotic cells and is distantly
related to the bacterial CorA Mg2+ transporter family. Here
we provide the first experimental evidence for the location of Alr1p in
the yeast plasma membrane and for the tight control of its expression
and turnover by Mg2+. Using well characterized
npi1 and end3 mutants deficient in the
endocytic pathway, we demonstrate that Alr1 protein turnover is
dependent on ubiquitination and endocytosis. Furthermore,
cells lacking the vacuolar protease Pep4p accumulated Alr1p in the
vacuole. Mutants lacking Alr1p (
The Yeast Plasma Membrane Protein Alr1 Controls
Mg2+ Homeostasis and Is Subject to
Mg2+-dependent Control of Its Synthesis and
Degradation*
§,§,,
,**
Vienna Biocenter, Institute of Microbiology
and Genetics, University of Vienna, A-1030 Vienna, the
¶ Biomembrane Research Center (SFB), Department of
Biochemistry, Technical University of Graz, A-8010 Graz, and the
Institute of Forest Ecology, University of Agricultural
Sciences, Vienna, A-1180 Vienna, Austria
alr1) showed
a 60% reduction of total intracellular Mg2+ compared with
the wild type and failed to grow in standard media. When starved
of Mg2+, mutant and wild-type cells had similar low levels
of intracellular Mg2+; but upon addition of
Mg2+, wild-type cells replenished the intracellular
Mg2+ pool within a few hours, whereas
alr1 mutant cells did not. Expression of the
bacterial Mg2+ transporter CorA in the yeast
alr1 mutant partially restored growth in
standard media. The results are discussed in terms of Alr1p being a
plasma membrane transporter with high selectivity for
Mg2+.
*
This work was supported by Austrian Science Fund
(FWF) Project F706, Austrian National Bank Project P7273,
European Union Grant BIO4-CT97-2294 (EUROFAN II), the Austrian Ministry
of Education, Science, and Culture EUROFAN II Supplement Project, and
AUSTROFAN Grant GZ 200.042/2-Pr/4/2000 (to S. D. K. and R. J. S.).The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. S. Moomaw and M. E. Maguire The Unique Nature of Mg2+ Channels Physiology, October 1, 2008; 23(5): 275 - 285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-G. Li, L. N. Sokolov, Y.-H. Yang, D.-P. Li, J. Ting, G. K. Pandy, and S. Luan A Mitochondrial Magnesium Transporter Functions in Arabidopsis Pollen Development Mol Plant, July 1, 2008; 1(4): 675 - 685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Payandeh, C. Li, M. Ramjeesingh, E. Poduch, C. E. Bear, and E. F. Pai Probing Structure-Function Relationships and Gating Mechanisms in the CorA Mg2+ Transport System J. Biol. Chem., April 25, 2008; 283(17): 11721 - 11733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Schumacher, I. F. de Larrinoa, and B. Tudzynski Calcineurin-Responsive Zinc Finger Transcription Factor CRZ1 of Botrytis cinerea Is Required for Growth, Development, and Full Virulence on Bean Plants Eukaryot. Cell, April 1, 2008; 7(4): 584 - 601. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wiesenberger, K. Steinleitner, R. Malli, W. F. Graier, J. Vormann, R. J. Schweyen, and J. A. Stadler Mg2+ Deprivation Elicits Rapid Ca2+ Uptake and Activates Ca2+/Calcineurin Signaling in Saccharomyces cerevisiae Eukaryot. Cell, April 1, 2007; 6(4): 592 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Uemura, K. Kashiwagi, and K. Igarashi Polyamine Uptake by DUR3 and SAM3 in Saccharomyces cerevisiae J. Biol. Chem., March 9, 2007; 282(10): 7733 - 7741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wongwisansri and P. J. Laybourn Disruption of Histone Deacetylase Gene RPD3 Accelerates PHO5 Activation Kinetics through Inappropriate Pho84p Recycling Eukaryot. Cell, August 1, 2005; 4(8): 1387 - 1395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. I. Wolf TRPM7: Channeling the Future of Cellular Magnesium Homeostasis? Sci. Signal., May 18, 2004; 2004(233): pe23 - pe23. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Muller, D. Gunzel, and W.-R. Schlue Activation of AMPA/Kainate Receptors but Not Acetylcholine Receptors Causes Mg2+ Influx into Retzius Neurones of the Leech Hirudo medicinalis J. Gen. Physiol., November 24, 2003; 122(6): 727 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Hicks, Z. Wang, Y. Wei, R. Kent, A. A. Guffanti, H. Banciu, D. H. Bechhofer, and T. A. Krulwich A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake PNAS, September 2, 2003; 100(18): 10213 - 10218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Haynes, C. Kung, Y. Saimi, and R. R. Preston An exchanger-like protein underlies the large Mg2+ current in Paramecium PNAS, November 26, 2002; 99(24): 15717 - 15722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Stadler and R. J. Schweyen The Yeast Iron Regulon Is Induced upon Cobalt Stress and Crucial for Cobalt Tolerance J. Biol. Chem., October 11, 2002; 277(42): 39649 - 39654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gregan, M. Kolisek, and R. J. Schweyen Mitochondrial Mg2+ homeostasis is critical for group II intron splicing in vivo Genes & Dev., September 1, 2001; 15(17): 2229 - 2237. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |