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Originally published In Press as doi:10.1074/jbc.M011059200 on January 30, 2001

J. Biol. Chem., Vol. 276, Issue 19, 16289-16295, May 11, 2001
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Polyamine Enhancement of the Synthesis of Adenylate Cyclase at the Translational Level and the Consequential Stimulation of the Synthesis of the RNA Polymerase sigma 28 Subunit*

Madoka YoshidaDagger , Keiko KashiwagiDagger , Gota Kawai§, Akira Ishihama, and Kazuei IgarashiDagger ||

From the Dagger  Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, the § Department of Industrial Chemistry, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino-shi, Chiba 275-8588, and the  Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411-0801, Japan

The effects of polyamines on the synthesis of various sigma  subunits of RNA polymerase were studied using Western blot analysis. Synthesis of sigma 28 was stimulated 4.0-fold and that of sigma 38 was stimulated 2.3-fold by polyamines, whereas synthesis of other sigma  subunits was not influenced by polyamines. Stimulation of sigma 28 synthesis was due to an increase in the level of cAMP, which occurred through polyamine stimulation of the synthesis of adenylate cyclase at the level of translation. Polyamines were found to increase the translation of adenylate cyclase mRNA by facilitating the UUG codon-dependent initiation. Analysis of RNA secondary structure suggests that exposure of the Shine-Dalgarno sequence of mRNA is a prerequisite for polyamine stimulation of the UUG codon-dependent initiation.

* This work was supported by a grant-in-aid for scientific research from the Ministry of Education, Science, Sports and Culture, Japan, and by Research for the Future Program Grant JSPS-RFTF 97L00503 from the Japan Society for the Promotion of Science.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed. Tel.: 81-43-290-2897; Fax: 81-43-290-2900; E-mail: iga16077@p.chiba-u.ac.jp.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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