JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M100119200 on February 9, 2001

J. Biol. Chem., Vol. 276, Issue 19, 16335-16340, May 11, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/19/16335    most recent
M100119200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kawar, Z.
Right arrow Articles by Jarvis, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kawar, Z.
Right arrow Articles by Jarvis, D. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Insect Cells Encode a Class II alpha -Mannosidase with Unique Properties*

Ziad KawarDagger , Khanita Karaveg§, Kelley W. Moremen§, and Donald L. JarvisDagger

From the Dagger  Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-3944 and the § Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602

Previously, we cloned and characterized an insect (Sf9) cell cDNA encoding a class II alpha -mannosidase with amino acid sequence and biochemical similarities to mammalian Golgi alpha -mannosidase II. Since then, it has been demonstrated that other mammalian class II alpha -mannosidases can participate in N-glycan processing. Thus, the present study was performed to evaluate the catalytic properties of the Sf9 class II alpha -mannosidase and to more clearly determine its relationship to mammalian Golgi alpha -mannosidase II. The results showed that the Sf9 enzyme is cobalt-dependent and can hydrolyze Man5GlcNAc2 to Man3GlcNAc2, but it cannot hydrolyze GlcNAcMan5GlcNAc2. These data establish that the Sf9 enzyme is distinct from Golgi alpha -mannosidase II. This enzyme is not a lysosomal alpha -mannosidase because it is not active at acidic pH and it is localized in the Golgi apparatus. In fact, its sensitivity to swainsonine distinguishes the Sf9 enzyme from all other known mammalian class II alpha -mannosidases that can hydrolyze Man5GlcNAc2. Based on these properties, we designated this enzyme Sf9 alpha -mannosidase III and concluded that it probably provides an alternate N-glycan processing pathway in Sf9 cells.

* This work was supported by National Institutes of Health Grants GM49734 (to D. L. J.) and GM47533 and RR05351 (to K.  W.  M.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

To whom correspondence should be addressed: Dept. of Molecular Biology, Univ. of Wyoming, P. O. Box 3944, Laramie, Wyoming 82071-3944. Tel.: 307-766-4282; Fax: 307-766-5098; E-mail: DLJarvis@ uwyo.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Gutternigg, D. Kretschmer-Lubich, K. Paschinger, D. Rendic, J. Hader, P. Geier, R. Ranftl, V. Jantsch, G. Lochnit, and I. B. H. Wilson
Biosynthesis of Truncated N-Linked Oligosaccharides Results from Non-orthologous Hexosaminidase-mediated Mechanisms in Nematodes, Plants, and Insects
J. Biol. Chem., September 21, 2007; 282(38): 27825 - 27840.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Paschinger, M. Hackl, M. Gutternigg, D. Kretschmer-Lubich, U. Stemmer, V. Jantsch, G. Lochnit, and I. B. H. Wilson
A Deletion in the Golgi {alpha}-Mannosidase II Gene of Caenorhabditis elegans Results in Unexpected Non-wild-type N-Glycan Structures
J. Biol. Chem., September 22, 2006; 281(38): 28265 - 28277.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Sarkar, P. A. Leventis, C. I. Silvescu, V. N. Reinhold, H. Schachter, and G. L. Boulianne
Null Mutations in Drosophila N-Acetylglucosaminyltransferase I Produce Defects in Locomotion and a Reduced Life Span
J. Biol. Chem., May 5, 2006; 281(18): 12776 - 12785.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Leonard, D. Rendic, C. Rabouille, I. B. H. Wilson, T. Preat, and F. Altmann
The Drosophila fused lobes Gene Encodes an N-Acetylglucosaminidase Involved in N-Glycan Processing
J. Biol. Chem., February 24, 2006; 281(8): 4867 - 4875.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Vadaie and D. L. Jarvis
Molecular Cloning and Functional Characterization of a Lepidopteran Insect {beta}4-N-Acetylgalactosaminyltransferase with Broad Substrate Specificity, a Functional Role in Glycoprotein Biosynthesis, and a Potential Functional Role in Glycolipid Biosynthesis
J. Biol. Chem., August 6, 2004; 279(32): 33501 - 33518.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
S. Kim, S. K. Hwang, R. A. Dwek, P. M. Rudd, Y. H. Ahn, E.-H. Kim, C. Cheong, S. I. Kim, N. S. Park, and S. M. Lee
Structural determination of the N-glycans of a lepidopteran arylphorin reveals the presence of a monoglucosylated oligosaccharide in the storage protein
Glycobiology, March 1, 2003; 13(3): 147 - 157.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
B. R. Francis, L. Paquin, C. Weinkauf, and D. L. Jarvis
Biosynthesis and processing of Spodoptera frugiperda{alpha}-mannosidase III
Glycobiology, June 1, 2002; 12(6): 369 - 377.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.