JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M009659200 on January 30, 2001

J. Biol. Chem., Vol. 276, Issue 19, 16511-16519, May 11, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/19/16511    most recent
M009659200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, C. M.
Right arrow Articles by Ho, C. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, C. M.
Right arrow Articles by Ho, C. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

A Plant Gene Encoding a Myb-like Protein That Binds Telomeric GGTTTAG Repeats in Vitro*

Chung Mong ChenDagger §, Chi Ting WangDagger , and Chia Hsing Ho

From the Dagger  Institute of Botany, Academia Sinica, Taipei 115 and the  Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan, Republic of China

A gene (AtTRP1) encoding a telomeric repeat-binding protein has been isolated from Arabidopsis thaliana. AtTRP1 is a single copy gene located on chromosome 5 of A. thaliana. The protein AtTRP1 encoded by this gene is not only homologous to the Myb DNA-binding motifs of other telomere-binding proteins but also is similar to several initiator-binding proteins in plants. Gel retardation assay revealed that the 115 residues on the C terminus of this protein, including the Myb motif, are sufficient for binding to the double-stranded plant telomeric sequence. The isolated DNA-binding domain of AtTRP1 recognizes each telomeric repeat centered on the sequence GGTTTAG. The almost full-length protein of AtTRP1 does not form any complex at all with the DNA fragments carrying four or fewer GGTTTAG repeats. However, it forms a complex with the sequence (GGTTTAG)8 more efficiently than with the sequence (GGTTTAG)5. These data suggest that the minimum length of a telomeric DNA for AtTRP1 binding consists of five GGTTTAG repeats and that the optimal AtTRP1 binding may require eight or more GGTTTAG repeats. It also implies that this protein AtTRP1 may bind in vivo primarily to the ends of plant chromosomes, which consist of long stretches of telomeric repeats.

* This work was supported by Grant NSC 88-2311-B-001-080 from the National Science Council and Academia Sinica in the Republic of China.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) ATH17722.

§ To whom correspondence should be addressed, Tel.: 886-2-27899590 (Ext. 216); Fax: 886-2-27827954; E-mail: bocmchen@ccvax.sinica.edu.tw.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. Ko, S.-H. Jun, H. Bae, J.-S. Byun, W. Han, H. Park, S. W. Yang, S.-Y. Park, Y. H. Jeon, C. Cheong, et al.
Structure of the DNA-binding domain of NgTRF1 reveals unique features of plant telomere-binding proteins
Nucleic Acids Res., May 1, 2008; 36(8): 2739 - 2755.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J.-P. Hong, M. Y. Byun, D.-H. Koo, K. An, J.-W. Bang, I. K. Chung, G. An, and W. T. Kim
Suppression of RICE TELOMERE BINDING PROTEIN1 Results in Severe and Gradual Developmental Defects Accompanied by Genome Instability in Rice
PLANT CELL, June 1, 2007; 19(6): 1770 - 1781.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Fang and D. L. Spector
Centromere Positioning and Dynamics in Living Arabidopsis Plants
Mol. Biol. Cell, December 1, 2005; 16(12): 5710 - 5718.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. V. Shakirov, Y. V. Surovtseva, N. Osbun, and D. E. Shippen
The Arabidopsis Pot1 and Pot2 Proteins Function in Telomere Length Homeostasis and Chromosome End Protection
Mol. Cell. Biol., September 1, 2005; 25(17): 7725 - 7733.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. W. Yang, S. K. Kim, and W. T. Kim
Perturbation of NgTRF1 Expression Induces Apoptosis-Like Cell Death in Tobacco BY-2 Cells and Implicates NgTRF1 in the Control of Telomere Length and Stability
PLANT CELL, December 1, 2004; 16(12): 3370 - 3385.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. N. Karamysheva, Y. V. Surovtseva, L. Vespa, E. V. Shakirov, and D. E. Shippen
A C-terminal Myb Extension Domain Defines a Novel Family of Double-strand Telomeric DNA-binding Proteins in Arabidopsis
J. Biol. Chem., November 12, 2004; 279(46): 47799 - 47807.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Q.-H. Zhu, K. Ramm, R. Shivakkumar, E. S. Dennis, and N. M. Upadhyaya
The ANTHER INDEHISCENCE1 Gene Encoding a Single MYB Domain Protein Is Involved in Anther Development in Rice
Plant Physiology, July 1, 2004; 135(3): 1514 - 1525.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
S. H. Yoshimura, H. Maruyama, F. Ishikawa, R. Ohki, and K. Takeyasu
Molecular mechanisms of DNA end-loop formation by TRF2
Genes Cells, March 1, 2004; 9(3): 205 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. O. Marian, S. J. Bordoli, M. Goltz, R. A. Santarella, L. P. Jackson, O. Danilevskaya, M. Beckstette, R. Meeley, and H. W. Bass
The Maize Single myb histone 1 Gene, Smh1, Belongs to a Novel Gene Family and Encodes a Protein That Binds Telomere DNA Repeats in Vitro
Plant Physiology, November 1, 2003; 133(3): 1336 - 1350.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. W. Yang, D. H. Kim, J. J. Lee, Y. J. Chun, J.-H. Lee, Y. J. Kim, I. K. Chung, and W. T. Kim
Expression of the Telomeric Repeat Binding Factor Gene NgTRF1 Is Closely Coordinated with the Cell Division Program in Tobacco BY-2 Suspension Culture Cells
J. Biol. Chem., June 6, 2003; 278(24): 21395 - 21407.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. E. Gallego, N. Jalut, and C. I. White
Telomerase Dependence of Telomere Lengthening in ku80 Mutant Arabidopsis
PLANT CELL, March 1, 2003; 15(3): 782 - 789.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
P. Bundock and P. Hooykaas
Severe Developmental Defects, Hypersensitivity to DNA-Damaging Agents, and Lengthened Telomeres in Arabidopsis MRE11 Mutants
PLANT CELL, October 1, 2002; 14(10): 2451 - 2462.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Bundock, H. van Attikum, and P. Hooykaas
Increased telomere length and hypersensitivity to DNA damaging agents in an Arabidopsis KU70 mutant
Nucleic Acids Res., August 1, 2002; 30(15): 3395 - 3400.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.