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Originally published In Press as doi:10.1074/jbc.M009609200 on February 5, 2001

J. Biol. Chem., Vol. 276, Issue 19, 16561-16566, May 11, 2001
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Cotranscription and Intergenic Splicing of Human P2Y11 and SSF1 Genes*

Didier CommuniDagger §, Nathalie Suarez-HuertaDagger , Danielle Dussossoy||, Pierre Savi**, and Jean-Marie Boeynaemsdagger dagger

From the Dagger  Institute of Interdisciplinary Research, School of Medicine, Université Libre de Bruxelles, 808 Route de Lennik, 1070 Brussels, Belgium, || Sanofi-Synthelabo, 371 Rue du professeur Joseph Blayac, 34084 Montpellier, France, ** Sanofi-Synthelabo, 195 Route d'Espagne, 31036 Toulouse, France, and the dagger dagger  Department of Medical Chemistry, Erasme Hospital, Université Libre de Bruxelles, 808 Route de Lennik, 1070 Brussels, Belgium

The P2Y11 receptor is an ATP receptor positively coupled to the cAMP and phosphoinositide pathways. Ssf1 is a Saccharomyces cerevisiae nuclear protein, which plays an important role in mating. The gene encoding the human orthologue of SSF1 is adjacent to the P2Y11 gene on chromosome 19. During the screening of placenta cDNA libraries, we isolated a chimeric clone resulting from the intergenic splicing between the P2Y11 and SSF1 genes. The fusion protein was stably expressed in CHO-K1 cells where it generated a cAMP response to ATP qualitatively indistinguishable from that of the P2Y11 receptor. According to both Western blotting and cAMP response, the expression of the fusion protein in the transfected cells was clearly lower than that of the P2Y11 receptor. Both P2Y11 and SSF1 probes detected a 5.6-kb messenger RNA with a similar pattern of intensity in each of 11 human tissues. The ubiquitous presence of chimeric transcripts and their up-regulation during granulocytic differentiation indicate that the transgenic splicing between the P2Y11 and the SSF1 genes is a common and regulated phenomenon. There are very few examples of intergenic splicing in mammalian cells, and this is the first case involving a G-protein-coupled receptor.

* This work was supported by an Action de Recherche Concertée of the Communauté Française de Belgique, by the Belgian Program on Interuniversity Poles of Attraction initiated by the Belgian State, Prime Minister's Office, Federal Service for Science, Technology, and Culture, by grants of the Fonds de la Recherche Scientifique Médicale, the Bekales Foundation, the Fonds Médical Reine Elisabeth and Boehringer Ingelheim, and Fonds Emile DEFAY.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AJ298334 and AJ300588.

§ Chargé de recherches of the FNRS. To whom correspondence should be addressed: Inst. of Interdisciplinary Research, Campus Erasme, Bld. C, 5th Floor (local C5-145), 808 Route de Lennik, 1070 Brussels, Belgium. Tel.: 32-2-555-41-59; Fax: 32-2-555-46-55; E-mail: communid@ulb.ac.be.

Supported by Euroscreen.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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