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Originally published In Press as doi:10.1074/jbc.M007442200 on October 19, 2000

J. Biol. Chem., Vol. 276, Issue 2, 1099-1106, January 12, 2001
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A Novel Mechanism of Cooperation between c-Kit and Erythropoietin Receptor
STEM CELL FACTOR INDUCES THE EXPRESSION OF Stat5 AND ERYTHROPOIETIN RECEPTOR, RESULTING IN EFFICIENT PROLIFERATION AND SURVIVAL BY ERYTHROPOIETIN*

Reuben KapurDagger and Lei Zhang

From the Herman B Wells Center for Pediatric Research, Department of Pediatrics, James Whitcomb Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, Indiana 46202

Optimal production of red cells in vivo requires collaboration between c-Kit, erythropoietin receptor (Epo-R), and GATA-1. However, the mechanism(s) of collaboration remain unclear. Utilizing an embryonic stem cell-derived erythroid progenitor cell line from mice deficient in GATA-1, we have examined the role of c-Kit and Epo-R in erythroid cell proliferation, survival, and differentiation. In the absence of GATA-1, we demonstrate an essential role for c-Kit in survival and proliferation of erythroid progenitors via the regulation of Bcl-2 expression. In addition, we demonstrate that Epo-R and Stat5 are regulated by a second, novel mechanism. We demonstrate that c-Kit stimulation by stem cell factor is essential for the maintenance of Epo-R and Stat5 protein expression, which results in significantly enhanced Bcl-xL induction and survival of erythroid progenitors in response to Epo stimulation. Restoration of GATA-1 function results in terminal erythroid maturation and up-regulation of Epo-R and Bcl-xL expression, leading also to significantly enhanced survival of terminally differentiating erythroid progenitors in the presence of only Epo. These results demonstrate that c-Kit and Epo-R have unique role(s) during distinct phases of erythroid maturation, and both stem cell factor and Epo contribute to the regulation of the Epo-R-Stat5-Bcl-xL pathway to ensure optimal survival, proliferation, and differentiation of erythroid progenitors.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Recipient of an American Society of Hematology Junior Faculty Scholar Award. To whom correspondence should be addressed: Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, Cancer Research Inst., 1044 W. Walnut St., Rm. 425, Indianapolis, IN 46202. Tel.: 317-274-4658; Fax: 317-274-8679; E-mail: rkapur@iupui.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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