|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 2, 1107-1113, January 12, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Transthyretin (TTR) acts physiologically in the
transport of retinol in the circulation. We previously reported the
generation and partial characterization of TTR-deficient
(TTR
Biochemical Basis for Depressed Serum Retinol Levels in
Transthyretin-deficient Mice*
,,§,
, and§**
Institute of Human Nutrition, the
§ Department of Medicine, and the ¶ Institute of
Cancer Research of the College of Physicians and Surgeons, Columbia
University, New York, New York 10032 and the Medical
Research Council, Hammersmith Hospital,
London W12 0NN, United Kingdom
) mice. TTR mice have very low
circulating levels of retinol and its specific transport protein,
retinol-binding protein (RBP). We have examined the biochemical basis
for the low plasma retinol-RBP levels. Cultured primary hepatocytes
isolated from wild type (WT) and TTR mice accumulated RBP
in their media to an identical degree, suggesting that RBP was being
secreted from the hepatocytes at the same rate. In vivo
experiments support this conclusion. For the first 11 h after
complete nephrectomy, the levels retinol and RBP rose in the
circulations of WT and TTR mice at nearly identical
rates. However, human retinol-RBP injected intravenously was more
rapidly cleared from the circulation (t1/2 = 0.5 h for TTR versus
t1/2 >6 h for WT) and accumulated faster in the
kidneys of TTR compared with WT mice. The rate of
infiltration of the retinol-RBP complex from the circulation to tissue
interstitial fluids was identical in both strains. Taken together,
these data indicate that low circulating retinol-RBP levels in
TTR mice arise from increased renal filtration of the
retinol-RBP complex.
*
This work was supported by Grants EY12858 and DK52444 from
the National Institutes of Health and grants from the United States Department of Agriculture.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Mody, T. E. Graham, Y. Tsuji, Q. Yang, and B. B. Kahn Decreased clearance of serum retinol-binding protein and elevated levels of transthyretin in insulin-resistant ob/ob mice Am J Physiol Endocrinol Metab, April 1, 2008; 294(4): E785 - E793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Feart, J Vallortigara, D Higueret, B Gatta, A Tabarin, V Enderlin, P Higueret, and V Pallet Decreased expression of retinoid nuclear receptor (RAR{alpha} and RAR{gamma}) mRNA determined by real-time quantitative RT-PCR in peripheral blood mononuclear cells of hypothyroid patients J. Mol. Endocrinol., June 1, 2005; 34(3): 849 - 858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Gieng, J. Raila, and F. J. Rosales Accumulation of retinol in the liver after prolonged hyporetinolemia in the vitamin A-sufficient rat J. Lipid Res., April 1, 2005; 46(4): 641 - 649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Zimmermann, R. Wegmuller, C. Zeder, N. Chaouki, and T. Torresani The Effects of Vitamin A Deficiency and Vitamin A Supplementation on Thyroid Function in Goitrous Children J. Clin. Endocrinol. Metab., November 1, 2004; 89(11): 5441 - 5447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Quadro, W. S. Blaner, L. Hamberger, P. M. Novikoff, S. Vogel, R. Piantedosi, M. E. Gottesman, and V. Colantuoni The role of extrahepatic retinol binding protein in the mobilization of retinoid stores J. Lipid Res., November 1, 2004; 45(11): 1975 - 1982. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Zhang, R. C. Bast Jr., Y. Yu, J. Li, L. J. Sokoll, A. J. Rai, J. M. Rosenzweig, B. Cameron, Y. Y. Wang, X.-Y. Meng, et al. Three Biomarkers Identified from Serum Proteomic Analysis for the Detection of Early Stage Ovarian Cancer Cancer Res., August 15, 2004; 64(16): 5882 - 5890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Quadro, W. S. Blaner, L. Hamberger, R. N. Van Gelder, S. Vogel, R. Piantedosi, P. Gouras, V. Colantuoni, and M. E. Gottesman Muscle Expression of Human Retinol-binding Protein (RBP). SUPPRESSION OF THE VISUAL DEFECT OF RBP KNOCKOUT MICE J. Biol. Chem., August 9, 2002; 277(33): 30191 - 30197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Shang, K. Lai, A. I. Packer, J. Paik, W. S. Blaner, M. de Morais Vieira, P. Gouras, and D. J. Wolgemuth Targeted disruption of the mouse cis-retinol dehydrogenase gene: visual and nonvisual functions J. Lipid Res., April 1, 2002; 43(4): 590 - 597. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |