JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M009435200 on October 20, 2000

J. Biol. Chem., Vol. 276, Issue 2, 1119-1126, January 12, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/2/1119    most recent
M009435200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Westmark, C. J.
Right arrow Articles by Malter, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Westmark, C. J.
Right arrow Articles by Malter, J. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Up-regulation of Nucleolin mRNA and Protein in Peripheral Blood Mononuclear Cells by Extracellular-regulated Kinase*

Cara J. Westmark and James S. MalterDagger

From the Institute on Aging and Department of Pathology and Laboratory Medicine, University of Wisconsin Medical School, Madison, Wisconsin 53792

The signal transduction pathways regulating nucleolin mRNA and protein production have yet to be elucidated. Peripheral blood mononuclear cells treated with phorbol 12-myristate 13-acetate showed steady state levels of nucleolin mRNA that were 2-2.5-fold greater than untreated control cells. The up-regulation of nucleolin mRNA was substantially repressed by U0126, a specific inhibitor that blocks phosphorylation of extracellular-regulated kinase (ERK). Calcium ionophores A23187 and ionomycin also activated ERK and substantially elevated nucleolin mRNA levels, demonstrating phorbol 12-myristate 13-acetate and calcium signaling converge on ERK. Drugs that affected protein kinase C, protein kinase A, and phospholipase C signal transduction pathways did not alter nucleolin mRNA levels significantly. The half-life of nucleolin mRNA increased from 1.8 h in resting cells to 3.2 h with phorbol ester activation, suggesting ERK-mediated posttranscriptional regulation. Concomitantly, full-length nucleolin protein was increased. The higher levels of nucleolin protein were accompanied by increased binding of a 70-kDa nucleolin fragment to the 29-base instability element in the 3'-untranslated region of amyloid precursor protein (APP) mRNA in gel mobility shift assays. Supplementation of rabbit reticulocyte lysate with nucleolin decreased APP mRNA stability and protein production. These data suggest ERK up-regulates nucleolin posttranscriptionally thereby controlling APP production.

* This work was supported by National Institutes of Health Grant RO1AG10675 (to J. S. M.) and NIA Research Service Award AG00213 from the National Institutes of Health (to C. J. W.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: University of Wisconsin Hospital and Clinics, Rm. K4/812, 600 Highland Ave., Madison, WI 53792. Tel.: 608-263-6043; Fax: 608-265-6215; E-mail: jsmalter@facstaff.wisc.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Xie, I. Kobayashi, T. Kiyoshima, H. Yamaza, J.-y. Honda, K. Takahashi, N. Enoki, A. Akamine, and H. Sakai
Functional Implication of Nucleolin in the Mouse First Molar Development
J. Biol. Chem., August 10, 2007; 282(32): 23275 - 23283.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. K. Slevin, F. Gourronc, and R. S. Hartley
ElrA binding to the 3'UTR of cyclin E1 mRNA requires polyadenylation elements
Nucleic Acids Res., April 1, 2007; 35(7): 2167 - 2176.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Otake, S. Soundararajan, T. K. Sengupta, E. A. Kio, J. C. Smith, M. Pineda-Roman, R. K. Stuart, E. K. Spicer, and D. J. Fernandes
Overexpression of nucleolin in chronic lymphocytic leukemia cells induces stabilization of bcl2 mRNA
Blood, April 1, 2007; 109(7): 3069 - 3075.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Wang, J. Zhang, S. Theel, J. J. Barb, P. J. Munson, and R. L. Danner
Nitric oxide activation of Erk1/2 regulates the stability and translation of mRNA transcripts containing CU-rich elements
Nucleic Acids Res., June 6, 2006; 34(10): 3044 - 3056.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. P. Huddleson, N. Ahmad, and J. B. Lingrel
Up-regulation of the KLF2 Transcription Factor by Fluid Shear Stress Requires Nucleolin
J. Biol. Chem., June 2, 2006; 281(22): 15121 - 15128.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
J. Fan, N. M. Heller, M. Gorospe, U. Atasoy, and C. Stellato
The role of post-transcriptional regulation in chemokine gene expression in inflammation and allergy
Eur. Respir. J., November 1, 2005; 26(5): 933 - 947.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Z. Xu, S. Di Marco, I. Gallouzi, M. Rola-Pleszczynski, and D. Radzioch
RNA-Binding Protein HuR Is Required for Stabilization of SLC11A1 mRNA and SLC11A1 Protein Expression
Mol. Cell. Biol., September 15, 2005; 25(18): 8139 - 8149.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
D. E. Sullivan, M. Ferris, D. Pociask, and A. R. Brody
Tumor Necrosis Factor-{alpha} Induces Transforming Growth Factor-{beta}1 Expression in Lung Fibroblasts Through the Extracellular Signal-Regulated Kinase Pathway
Am. J. Respir. Cell Mol. Biol., April 1, 2005; 32(4): 342 - 349.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
Y. Otake, T. K. Sengupta, S. Bandyopadhyay, E. K. Spicer, and D. J. Fernandes
Retinoid-Induced Apoptosis in HL-60 Cells Is Associated with Nucleolin Down-Regulation and Destabilization of Bcl-2 mRNA
Mol. Pharmacol., January 1, 2005; 67(1): 319 - 326.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
C. Stellato
Post-transcriptional and Nongenomic Effects of Glucocorticoids
Proceedings of the ATS, November 1, 2004; 1(3): 255 - 263.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. F. Sinclair and A. D. O'Brien
Intimin Types {alpha}, {beta}, and {gamma} Bind to Nucleolin with Equivalent Affinity but Lower Avidity than to the Translocated Intimin Receptor
J. Biol. Chem., August 6, 2004; 279(32): 33751 - 33758.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Singh, J. Laughlin, P. A. Kosinski, and L. R. Covey
Nucleolin Is a Second Component of the CD154 mRNA Stability Complex That Regulates mRNA Turnover in Activated T Cells
J. Immunol., July 15, 2004; 173(2): 976 - 985.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. K. Sengupta, S. Bandyopadhyay, D. J. Fernandes, and E. K. Spicer
Identification of Nucleolin as an AU-rich Element Binding Protein Involved in bcl-2 mRNA Stabilization
J. Biol. Chem., March 19, 2004; 279(12): 10855 - 10863.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Esnault and J. S. Malter
Hyaluronic Acid or TNF-{alpha} Plus Fibronectin Triggers Granulocyte Macrophage-Colony-Stimulating Factor mRNA Stabilization in Eosinophils Yet Engages Differential Intracellular Pathways and mRNA Binding Proteins
J. Immunol., December 15, 2003; 171(12): 6780 - 6787.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. Hasler, D. Mordasini, M. Bianchi, A. Vandewalle, E. Feraille, and P.-Y. Martin
Dual Influence of Aldosterone on AQP2 Expression in Cultured Renal Collecting Duct Principal Cells
J. Biol. Chem., June 6, 2003; 278(24): 21639 - 21648.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
T. Yanagita, H. Kobayashi, Y. Uezono, H. Yokoo, T. Sugano, T. Saitoh, S.-I. Minami, S. Shiraishi, and A. Wada
Destabilization of Nav1.7 Sodium Channel alpha -Subunit mRNA by Constitutive Phosphorylation of Extracellular Signal-Regulated Kinase: Negative Regulation of Steady-State Level of Cell Surface Functional Sodium Channels in Adrenal Chromaffin Cells
Mol. Pharmacol., May 1, 2003; 63(5): 1125 - 1136.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. Wang, J. Fan, X. Yang, S. Furer-Galban, I. Lopez de Silanes, C. von Kobbe, J. Guo, S. N. Georas, F. Foufelle, D. G. Hardie, et al.
AMP-Activated Kinase Regulates Cytoplasmic HuR
Mol. Cell. Biol., May 15, 2002; 22(10): 3425 - 3436.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Esnault and J. S. Malter
Extracellular signal-regulated kinase mediates granulocyte-macrophage colony-stimulating factor messenger RNA stabilization in tumor necrosis factor-alpha plus fibronectin-activated peripheral blood eosinophils
Blood, May 13, 2002; 99(11): 4048 - 4052.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Yang and F. Carrier
The UV-inducible RNA-binding Protein A18 (A18 hnRNP) Plays a Protective Role in the Genotoxic Stress Response
J. Biol. Chem., December 7, 2001; 276(50): 47277 - 47284.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.