JBC Ideal method for primary cell transfection

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M008377200 on October 30, 2000

J. Biol. Chem., Vol. 276, Issue 2, 1138-1145, January 12, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/2/1138    most recent
M008377200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nguyên, D.-T.
Right arrow Articles by Raymond, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nguyên, D.-T.
Right arrow Articles by Raymond, M.

Multiple Yap1p-binding Sites Mediate Induction of the Yeast Major Facilitator FLR1 Gene in Response to Drugs, Oxidants, and Alkylating Agents*

Duc-Thang Nguyên, Anne-Marie Alarco, and Martine RaymondDagger

From the Institut de Recherches Cliniques de Montréal, Montréal, Québec, H2W 1R7, Canada

The bZip transcription factor Yap1p plays an important role in oxidative stress response and multidrug resistance in Saccharomyces cerevisiae. We have previously demonstrated that the FLR1 gene, encoding a multidrug transporter of the major facilitator superfamily, is a transcriptional target of Yap1p. The FLR1 promoter contains three potential Yap1p response elements (YREs) at positions -148 (YRE1), -167 (YRE2), and -364 (YRE3). To address the function of these YREs, the three sites have been individually mutated and tested in transactivation assays. Our results show that (i) each of the three YREs is functional and important for the optimal transactivation of FLR1 by Yap1p and that (ii) the three YREs are not functionally equivalent, mutation of YRE3 being the most deleterious, followed by YRE2 and YRE1. Simultaneous mutation of the three YREs abolished transactivation of the promoter by Yap1p, demonstrating that the three sites are essential for the regulation of FLR1 by Yap1p. Gel retardation assays confirmed that Yap1p differentially binds to the three YREs (YRE3 > YRE2 > YRE1). We show that the transcription of FLR1 is induced upon cell treatment with the oxidizing agents diamide, diethylmaleate, hydrogen peroxide, and tert-butyl hydroperoxide, the antimitotic drug benomyl, and the alkylating agent methylmethane sulfonate and that this induction is mediated by Yap1p through the three YREs. Finally, we show that FLR1 overexpression confers resistance to diamide, diethylmaleate, and menadione but hypersensitivity to H2O2, demonstrating that the Flr1p transporter participates in Yap1p-mediated oxidative stress response in S. cerevisiae.

* This work was supported by Medical Research Council of Canada Grant MT-15679 (to M. R.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Supported by a scholarship from le Fonds de la Recherche en Santé du Québec (FRSQ). To whom correspondence should be addressed: Institut de Recherches Cliniques de Montréal, 110 Pine Ave. West, Montréal, Québec, Canada H2W 1R7. Tel.: 514-987-5770; Fax: 514-987-5764; E-mail: raymonm@ircm.qc.ca.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Omberg, G. H. Golub, and O. Alter
A tensor higher-order singular value decomposition for integrative analysis of DNA microarray data from different studies
PNAS, November 20, 2007; 104(47): 18371 - 18376.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
B. Rognon, Z. Kozovska, A. T. Coste, G. Pardini, and D. Sanglard
Identification of promoter elements responsible for the regulation of MDR1 from Candida albicans, a major facilitator transporter involved in azole resistance
Microbiology, December 1, 2006; 152(12): 3701 - 3722.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Romero, P. Desai, N. DeLillo, and A. Vancura
Expression of FLR1 Transporter Requires Phospholipase C and Is Repressed by Mediator
J. Biol. Chem., March 3, 2006; 281(9): 5677 - 5685.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
T. Srikantha, R. Zhao, K. Daniels, J. Radke, and D. R. Soll
Phenotypic Switching in Candida glabrata Accompanied by Changes in Expression of Genes with Deduced Functions in Copper Detoxification and Stress
Eukaryot. Cell, August 1, 2005; 4(8): 1434 - 1445.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J. B. Harry, B. G. Oliver, J. L. Song, P. M. Silver, J. T. Little, J. Choiniere, and T. C. White
Drug-Induced Regulation of the MDR1 Promoter in Candida albicans
Antimicrob. Agents Chemother., July 1, 2005; 49(7): 2785 - 2792.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Lucau-Danila, G. Lelandais, Z. Kozovska, V. Tanty, T. Delaveau, F. Devaux, and C. Jacq
Early Expression of Yeast Genes Affected by Chemical Stress
Mol. Cell. Biol., March 1, 2005; 25(5): 1860 - 1868.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. Lev, R. Hadar, P. Amedeo, S. E. Baker, O. C. Yoder, and B. A. Horwitz
Activation of an AP1-Like Transcription Factor of the Maize Pathogen Cochliobolus heterostrophus in Response to Oxidative Stress and Plant Signals
Eukaryot. Cell, February 1, 2005; 4(2): 443 - 454.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
P. M. Silver, B. G. Oliver, and T. C. White
Role of Candida albicans Transcription Factor Upc2p in Drug Resistance and Sterol Metabolism
Eukaryot. Cell, December 1, 2004; 3(6): 1391 - 1397.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Maeta, S. Izawa, S. Okazaki, S. Kuge, and Y. Inoue
Activity of the Yap1 Transcription Factor in Saccharomyces cerevisiae Is Modulated by Methylglyoxal, a Metabolite Derived from Glycolysis
Mol. Cell. Biol., October 1, 2004; 24(19): 8753 - 8764.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Wysocki, P.-K. Fortier, E. Maciaszczyk, M. Thorsen, A. Leduc, A. Odhagen, G. Owsianik, S. Ulaszewski, D. Ramotar, and M. J. Tamas
Transcriptional Activation of Metalloid Tolerance Genes in Saccharomyces cerevisiae Requires the AP-1-like Proteins Yap1p and Yap8p
Mol. Biol. Cell, May 1, 2004; 15(5): 2049 - 2060.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
C. Gauthier, S. Weber, A.-M. Alarco, O. Alqawi, R. Daoud, E. Georges, and M. Raymond
Functional Similarities and Differences between Candida albicans Cdr1p and Cdr2p Transporters
Antimicrob. Agents Chemother., May 1, 2003; 47(5): 1543 - 1554.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Hikkel, A. Lucau-Danila, T. Delaveau, P. Marc, F. Devaux, and C. Jacq
A General Strategy to Uncover Transcription Factor Properties Identifies a New Regulator of Drug Resistance in Yeast
J. Biol. Chem., March 21, 2003; 278(13): 11427 - 11432.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Le Crom, F. Devaux, P. Marc, X. Zhang, W. S. Moye-Rowley, and C. Jacq
New Insights into the Pleiotropic Drug Resistance Network from Genome-Wide Characterization of the YRR1 Transcription Factor Regulation System
Mol. Cell. Biol., April 15, 2002; 22(8): 2642 - 2649.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.