| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 2, 1424-1433, January 12, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Ammonium transporters play a key functional role
in nitrogen uptake and assimilation in microorganisms and plants;
however, little is known about their structural counterpart in mammals. Here, we report the molecular cloning and biochemical characterization of Rh type B glycoproteins, human RhBG and
mouse Rhbg, two new members of the Rh family with distinct tissue
specificities. The RhBG orthologues possess a conserved
12-transmembrane topology and most resemble bacterial and archaeal
ammonium transporters. Human RHBG resides at chromosome
1q21.3, which harbors candidate genes for medullary cystic kidney
disease, whereas mouse Rhbg is syntenic on chromosome 3. Northern blot and in situ hybridization revealed that
RHBG and Rhbg are predominantly expressed in
liver, kidney, and skin, the specialized organs involving ammonia
genesis, excretion, or secretion. Confocal microscopy showed
that RhBG is located in the plasma membrane and in some intracellular
granules. Western blots of membrane proteins from stable HEK293 cells
and from mouse kidney and liver confirmed this distribution.
N-Glycanase digestion showed that RhBG/Rhbg has a
carbohydrate moiety probably attached at the NHS motif on exoloop 1. Phylogenetic clustering, tissue-specific expression, and plasma
membrane location suggest that RhBG homologous proteins are the long
sought major ammonium transporters in mammalians.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF193807 and AF193808.
Rh Type B Glycoprotein Is a New Member of the Rh Superfamily and
a Putative Ammonia Transporter in Mammals*
,,¶
Laboratory of Biochemistry and Molecular Genetics,
Lindsley F. Kimball Research Institute, New York Blood Center, New
York, New York 10021 and the § Program in Developmental Biology, the
Hospital for Sick Children and Department of Molecular and Medical
Genetics, University of Toronto, Ontario M5G 1X8, Canada
*
This work was supported by Institutional Fund D33210101 of
the New York Blood Center and in part by National Institutes of Health
Grant HL54459 (to C.-H.H).The costs of publication of this article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Sohet, Y. Colin, S. Genetet, P. Ripoche, S. Metral, C. Le Van Kim, and C. Lopez Phosphorylation and Ankyrin-G Binding of the C-terminal Domain Regulate Targeting and Function of the Ammonium Transporter RhBG J. Biol. Chem., September 26, 2008; 283(39): 26557 - 26567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Worrell, L. Merk, and J. B. Matthews Ammonium transport in the colonic crypt cell line, T84: role for Rhesus glycoproteins and NKCC1 Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G429 - G440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Nawata, C. C. Y. Hung, T. K. N. Tsui, J. M. Wilson, P. A. Wright, and C. M. Wood Ammonia excretion in rainbow trout (Oncorhynchus mykiss): evidence for Rh glycoprotein and H+-ATPase involvement Physiol Genomics, November 14, 2007; 31(3): 463 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Kim, C. Baylis, J. W. Verlander, K.-H. Han, S. Reungjui, M. E. Handlogten, and I. D. Weiner Effect of reduced renal mass on renal ammonia transporter family, Rh C glycoprotein and Rh B glycoprotein, expression Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1238 - F1247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Y. C. Hung, K. N. T. Tsui, J. M. Wilson, C. M. Nawata, C. M. Wood, and P. A. Wright Rhesus glycoprotein gene expression in the mangrove killifish Kryptolebias marmoratus exposed to elevated environmental ammonia levels and air J. Exp. Biol., July 15, 2007; 210(14): 2419 - 2429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Khademi and R. M. Stroud The Amt/MEP/Rh Family: Structure of AmtB and the Mechanism of Ammonia Gas Conduction. Physiology, December 1, 2006; 21(6): 419 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-H. Han, B. P. Croker, W. L. Clapp, D. Werner, M. Sahni, J. Kim, H.-Y. Kim, M. E. Handlogten, and I. D. Weiner Expression of the Ammonia Transporter, Rh C Glycoprotein, in Normal and Neoplastic Human Kidney J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2670 - 2679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yang, D. Zhao, E. Solenov, and A. S. Verkman Evidence from knockout mice against physiologically significant aquaporin 8-facilitated ammonia transport Am J Physiol Cell Physiol, September 1, 2006; 291(3): C417 - C423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Ji, S. Hashmi, Z. Liu, J. Zhang, Y. Chen, and C.-H. Huang CeRh1 (rhr-1) is a dominant Rhesus gene essential for embryonic development and hypodermal function in Caenorhabditis elegans PNAS, April 11, 2006; 103(15): 5881 - 5886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-O. D. Mak, B. Dang, I. D. Weiner, J. K. Foskett, and C. M. Westhoff Characterization of ammonia transport by the kidney Rh glycoproteins RhBG and RhCG Am J Physiol Renal Physiol, February 1, 2006; 290(2): F297 - F305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Seshadri, J. D. Klein, S. Kozlowski, J. M. Sands, Y.-H. Kim, K.-H. Han, M. E. Handlogten, J. W. Verlander, and I. D. Weiner Renal expression of the ammonia transporters, Rhbg and Rhcg, in response to chronic metabolic acidosis Am J Physiol Renal Physiol, February 1, 2006; 290(2): F397 - F408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Huang and J. Peng Evolutionary conservation and diversification of Rh family genes and proteins PNAS, October 25, 2005; 102(43): 15512 - 15517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Handlogten, S.-P. Hong, L. Zhang, A. W. Vander, M. L. Steinbaum, M. Campbell-Thompson, and I. D. Weiner Expression of the ammonia transporter proteins Rh B glycoprotein and Rh C glycoprotein in the intestinal tract Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G1036 - G1047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lopez, S. Metral, D. Eladari, S. Drevensek, P. Gane, R. Chambrey, V. Bennett, J.-P. Cartron, C. Le Van Kim, and Y. Colin The Ammonium Transporter RhBG: REQUIREMENT OF A TYROSINE-BASED SIGNAL AND ANKYRIN-G FOR BASOLATERAL TARGETING AND MEMBRANE ANCHORAGE IN POLARIZED KIDNEY EPITHELIAL CELLS J. Biol. Chem., March 4, 2005; 280(9): 8221 - 8228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Nakhoul, H. DeJong, S. M. Abdulnour-Nakhoul, E. L. Boulpaep, K. Hering-Smith, and L. L. Hamm Characteristics of renal Rhbg as an NH4+ transporter Am J Physiol Renal Physiol, January 1, 2005; 288(1): F170 - F181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zheng, D. Kostrewa, S. Berneche, F. K. Winkler, and X.-D. Li The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli PNAS, December 7, 2004; 101(49): 17090 - 17095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Ripoche, O. Bertrand, P. Gane, C. Birkenmeier, Y. Colin, and J.-P. Cartron Human Rhesus-associated glycoprotein mediates facilitated transport of NH3 into red blood cells PNAS, December 7, 2004; 101(49): 17222 - 17227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Wagner, K. E. Finberg, S. Breton, V. Marshansky, D. Brown, and J. P. Geibel Renal Vacuolar H+-ATPase Physiol Rev, October 1, 2004; 84(4): 1263 - 1314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Handlogten, S.-P. Hong, C. M. Westhoff, and I. D. Weiner Basolateral ammonium transport by the mouse inner medullary collecting duct cell (mIMCD-3) Am J Physiol Renal Physiol, October 1, 2004; 287(4): F628 - F638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cheval, J. P. Duong Van Huyen, P. Bruneval, J.-M. Verbavatz, J.-M. Elalouf, and A. Doucet Plasticity of mouse renal collecting duct in response to potassium depletion Physiol Genomics, September 16, 2004; 19(1): 61 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Ludewig Electroneutral ammonium transport by basolateral rhesus B glycoprotein J. Physiol., September 15, 2004; 559(3): 751 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. K. Johansson, J. Brodd, C. Eklof, M. Ferletta, G. Hesselager, C.-F. Tiger, L. Uhrbom, and B. Westermark Identification of candidate cancer-causing genes in mouse brain tumors by retroviral tagging PNAS, August 3, 2004; 101(31): 11334 - 11337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Westhoff, D. L. Siegel, C. G. Burd, and J. K. Foskett Mechanism of Genetic Complementation of Ammonium Transport in Yeast by Human Erythrocyte Rh-associated Glycoprotein J. Biol. Chem., April 23, 2004; 279(17): 17443 - 17448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Bakouh, F. Benjelloun, P. Hulin, F. Brouillard, A. Edelman, B. Cherif-Zahar, and G. Planelles NH3 Is Involved in the NH4 Transport Induced by the Functional Expression of the Human Rh C Glycoprotein J. Biol. Chem., April 16, 2004; 279(16): 15975 - 15983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Quentin, D. Eladari, L. Cheval, C. Lopez, D. Goossens, Y. Colin, J.-P. Cartron, M. Paillard, and R. Chambrey RhBG and RhCG, the Putative Ammonia Transporters, Are Expressed in the Same Cells in the Distal Nephron J. Am. Soc. Nephrol., March 1, 2003; 14(3): 545 - 554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Verlander, R. T. Miller, A. E. Frank, I. E. Royaux, Y.-H. Kim, and I. D. Weiner Localization of the ammonium transporter proteins RhBG and RhCG in mouse kidney Am J Physiol Renal Physiol, February 1, 2003; 284(2): F323 - F337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Eladari, L. Cheval, F. Quentin, O. Bertrand, I. Mouro, B. Cherif-Zahar, J.-P. Cartron, M. Paillard, A. Doucet, and R. Chambrey Expression of RhCG, a New Putative NH3/NH4+ Transporter, along the Rat Nephron J. Am. Soc. Nephrol., August 1, 2002; 13(8): 1999 - 2008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Patriarca, R. Tate, and M. Iaccarino Key Role of Bacterial NH4+ Metabolism in Rhizobium-Plant Symbiosis Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 203 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Soupene, N. King, E. Feild, P. Liu, K. K. Niyogi, C.-H. Huang, and S. Kustu Rhesus expression in a green alga is regulated by CO2 PNAS, May 28, 2002; 99(11): 7769 - 7773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Ludewig, N. von Wiren, and W. B. Frommer Uniport of NH4+ by the Root Hair Plasma Membrane Ammonium Transporter LeAMT1;1 J. Biol. Chem., April 12, 2002; 277(16): 13548 - 13555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Westhoff, M. Ferreri-Jacobia, D.-O. D. Mak, and J. K. Foskett Identification of the Erythrocyte Rh Blood Group Glycoprotein as a Mammalian Ammonium Transporter J. Biol. Chem., April 5, 2002; 277(15): 12499 - 12502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Garratty, M. J. Telen, and L. D. Petz Red Cell Antigens as Functional Molecules and Obstacles to Transfusion Hematology, January 1, 2002; 2002(1): 445 - 462. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
