| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J. Biol. Chem., Vol. 276, Issue 20, 16649-16654, May 18, 2001
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
From the Department of Medicine, Section of Hematology/Oncology,
University of Chicago, Chicago, Illinois 60637
We previously demonstrated that activation of the
glucocorticoid receptor (GR) initiates an antiapoptotic signal in the
immortalized human mammary epithelial cell line MCF10A that is
dependent on the GR's transcriptional activity. In this study, we show
that the survival role of GR activation extends to protecting human breast cancer cells undergoing apoptosis after growth factor
deprivation. Serum and glucocorticoid-regulated kinase-1
(sgk), a gene previously identified as a direct
transcriptional target of the activated GR in a rat mammary tumor cell
line, was rapidly induced after GR activation in human mammary
epithelial cells. Furthermore, in the absence of all growth factors,
ectopic sgk expression inhibited apoptosis, suggesting that
SGK is a survival kinase. Finally, kinase-dead SGK expression inhibited
the protection from apoptosis usually seen after GR activation. These
findings suggest that SGK is an important downstream target of
GR-mediated survival signaling and that it is distinct from other
survival kinases because it can be primarily regulated at the level of transcription.
Glucocorticoid Receptor-mediated Protection from Apoptosis Is
Associated with Induction of the Serine/Threonine Survival Kinase
Gene, sgk-1*
*
This work was supported in part by National Institutes of
Health Grant R21 CA66132 (NCI), The National Women's Cancer Research Alliance, a Cancer Research Foundation Young Investigator's Award (to
S. D. C.), and The Susan G. Komen Foundation.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed: Dept. of Medicine,
University of Chicago, 5841 South Maryland Ave., MC 2115, Chicago IL
60637. Tel.: 773-834-2604; Fax: 773-834-0188; E-mail:
sconzen@medicine.bsd.uchicago.edu.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. D. Conzen Minireview: Nuclear Receptors and Breast Cancer Mol. Endocrinol., October 1, 2008; 22(10): 2215 - 2228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dehner, M. Hadjihannas, J. Weiske, O. Huber, and J. Behrens Wnt Signaling Inhibits Forkhead Box O3a-induced Transcription and Apoptosis through Up-regulation of Serum- and Glucocorticoid-inducible Kinase 1 J. Biol. Chem., July 11, 2008; 283(28): 19201 - 19210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Belova, D. R. Brickley, B. Ky, S. K. Sharma, and S. D. Conzen Hsp90 Regulates the Phosphorylation and Activity of Serum- and Glucocorticoid-regulated Kinase-1 J. Biol. Chem., July 4, 2008; 283(27): 18821 - 18831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Robbins, H. Critchlow, A. Lloyd, J. Cilia, J. Clarke, B. Bond, D. Jones, and P. Maycox Differential expression of IEG mRNA in rat brain following acute treatment with clozapine or haloperidol: a semi-quantitative RT--PCR study J Psychopharmacol, July 1, 2008; 22(5): 536 - 542. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Pew, M. Zou, D. R. Brickley, and S. D. Conzen Glucocorticoid (GC)-Mediated Down-Regulation of Urokinase Plasminogen Activator Expression via the Serum and GC Regulated Kinase-1/Forkhead Box O3a Pathway Endocrinology, May 1, 2008; 149(5): 2637 - 2645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D Conzen Environmental Stress and the Neuroendocrine Response: Is There a Cancer Connection? Am. Assoc. Cancer Res. Educ. Book, April 12, 2008; 2008(1): 255 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Zhao, R. Lehr, A. M. Smallwood, T. F. Ho, K. Maley, T. Randall, M. S. Head, K. K. Koretke, and C. G. Schnackenberg Crystal structure of the kinase domain of serum and glucocorticoid-regulated kinase 1 in complex with AMP PNP Protein Sci., December 1, 2007; 16(12): 2761 - 2769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Martel, D. Michael, G. Fejes-Toth, and A. Naray-Fejes-Toth Melanophilin, a novel aldosterone-induced gene in mouse cortical collecting duct cells Am J Physiol Renal Physiol, September 1, 2007; 293(3): F904 - F913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Huang and D. J. Tindall Dynamic FoxO transcription factors J. Cell Sci., August 1, 2007; 120(15): 2479 - 2487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Pao, J. A. McCormick, H. Li, J. Siu, C. Govaerts, V. Bhalla, R. Soundararajan, and D. Pearce NH2 terminus of serum and glucocorticoid-regulated kinase 1 binds to phosphoinositides and is essential for isoform-specific physiological functions Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1741 - F1750. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Cordas, A. Naray-Fejes-Toth, and G. Fejes-Toth Subcellular location of serum- and glucocorticoid-induced kinase-1 in renal and mammary epithelial cells Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1971 - C1981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. E. Tissing, M. L. den Boer, J. P. P. Meijerink, R. X. Menezes, S. Swagemakers, P. J. van der Spek, S. E. Sallan, S. A. Armstrong, and R. Pieters Genomewide identification of prednisolone-responsive genes in acute lymphoblastic leukemia cells Blood, May 1, 2007; 109(9): 3929 - 3935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Tomlinson, A. Boudreau, D. Wu, E. Atlas, and R. J. G. Hache Modulation of Early Human Preadipocyte Differentiation by Glucocorticoids Endocrinology, November 1, 2006; 147(11): 5284 - 5293. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Lang, C. Bohmer, M. Palmada, G. Seebohm, N. Strutz-Seebohm, and V. Vallon (Patho)physiological Significance of the Serum- and Glucocorticoid-Inducible Kinase Isoforms. Physiol Rev, October 1, 2006; 86(4): 1151 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wu, M. Zou, D. R. Brickley, T. Pew, and S. D. Conzen Glucocorticoid Receptor Activation Signals through Forkhead Transcription Factor 3a in Breast Cancer Cells Mol. Endocrinol., October 1, 2006; 20(10): 2304 - 2314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Kobayashi, J. M. Voyich, A. R. Whitney, and F. R. DeLeo Spontaneous neutrophil apoptosis and regulation of cell survival by granulocyte macrophage-colony stimulating factor J. Leukoc. Biol., December 1, 2005; 78(6): 1408 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cobb and D. Duboule Comparative analysis of genes downstream of the Hoxd cluster in developing digits and external genitalia Development, July 1, 2005; 132(13): 3055 - 3067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. McClintock, S. D. Conzen, S. Gehlert, C. Masi, and F. Olopade Mammary Cancer and Social Interactions: Identifying Multiple Environments That Regulate Gene Expression Throughout the Life Span J. Gerontol. B. Psychol. Sci. Soc. Sci., March 1, 2005; 60(suppl_Special_Issue_1): 32 - 41. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wu, T. Pew, M. Zou, D. Pang, and S. D. Conzen Glucocorticoid Receptor-induced MAPK Phosphatase-1 (MPK-1) Expression Inhibits Paclitaxel-associated MAPK Activation and Contributes to Breast Cancer Cell Survival J. Biol. Chem., February 11, 2005; 280(6): 4117 - 4124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhou and P. M. Snyder Nedd4-2 Phosphorylation Induces Serum and Glucocorticoid-regulated Kinase (SGK) Ubiquitination and Degradation J. Biol. Chem., February 11, 2005; 280(6): 4518 - 4523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Wintermantel, D. Bock, V. Fleig, E. F. Greiner, and G. Schutz The Epithelial Glucocorticoid Receptor Is Required for the Normal Timing of Cell Proliferation during Mammary Lobuloalveolar Development but Is Dispensable for Milk Production Mol. Endocrinol., February 1, 2005; 19(2): 340 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang, R. Cui, X. Cheng, and J. Du Antiapoptotic Effect of Serum and Glucocorticoid-Inducible Protein Kinase Is Mediated by Novel Mechanism Activating I{kappa}B Kinase Cancer Res., January 15, 2005; 65(2): 457 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ueda, S. Wang, N. Dumont, J. Y. Yi, Y. Koh, and C. L. Arteaga Overexpression of HER2 (erbB2) in Human Breast Epithelial Cells Unmasks Transforming Growth Factor {beta}-induced Cell Motility J. Biol. Chem., June 4, 2004; 279(23): 24505 - 24513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wu, S. Chaudhuri, D. R. Brickley, D. Pang, T. Karrison, and S. D. Conzen Microarray Analysis Reveals Glucocorticoid-Regulated Survival Genes That Are Associated With Inhibition of Apoptosis in Breast Epithelial Cells Cancer Res., March 1, 2004; 64(5): 1757 - 1764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M Wasserman and J. N Topper Adaptation of the endothelium to fluid flow: in vitro analyses of gene expression and in vivo implications Vascular Medicine, February 1, 2004; 9(1): 35 - 45. [Abstract] [PDF]
|
|
|
|
|
|
G. Ghannam, A. Takeda, T. Camarata, M. A. Moore, A. Viale, and N. R. Yaseen The Oncogene Nup98-HOXA9 Induces Gene Transcription in Myeloid Cells J. Biol. Chem., January 9, 2004; 279(2): 866 - 875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. De Bosscher, W. Vanden Berghe, and G. Haegeman The Interplay between the Glucocorticoid Receptor and Nuclear Factor-{kappa}B or Activator Protein-1: Molecular Mechanisms for Gene Repression Endocr. Rev., August 1, 2003; 24(4): 488 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Herr, E. Ucur, K. Herzer, S. Okouoyo, R. Ridder, P. H. Krammer, M. von Knebel Doeberitz, and K.-M. Debatin Glucocorticoid Cotreatment Induces Apoptosis Resistance toward Cancer Therapy in Carcinomas Cancer Res., June 15, 2003; 63(12): 3112 - 3120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Gaudet, K.-S. Huang, Y. Zhang, W. Huang, D. Mark, and J. R. Sportsman A Homogeneous Fluorescence Polarization Assay Adaptable for a Range of Protein Serine/Threonine and Tyrosine Kinases J Biomol Screen, April 1, 2003; 8(2): 164 - 175. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Farjah, B. P. Roxas, D. L. Geenen, and R. S. Danziger Dietary Salt Regulates Renal SGK1 Abundance: Relevance to Salt Sensitivity in the Dahl Rat Hypertension, April 1, 2003; 41(4): 874 - 878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Helms, G. Fejes-Toth, and A. Naray-Fejes-Toth Hormone-regulated transepithelial Na+ transport in mammalian CCD cells requires SGK1 expression Am J Physiol Renal Physiol, March 1, 2003; 284(3): F480 - F487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. Maiyar, M. L.L. Leong, and G. L. Firestone Importin-alpha Mediates the Regulated Nuclear Targeting of Serum- and Glucocorticoid-inducible Protein Kinase (Sgk) by Recognition of a Nuclear Localization Signal in the Kinase Central Domain Mol. Biol. Cell, March 1, 2003; 14(3): 1221 - 1239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. L. Leong, A. C. Maiyar, B. Kim, B. A. O'Keeffe, and G. L. Firestone Expression of the Serum- and Glucocorticoid-inducible Protein Kinase, Sgk, Is a Cell Survival Response to Multiple Types of Environmental Stress Stimuli in Mammary Epithelial Cells J. Biol. Chem., February 14, 2003; 278(8): 5871 - 5882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chun, T. Kwon, D. J. Kim, I. Park, G. Chung, E. J. Lee, S. K. Hong, S.-I. Chang, H. Y. Kim, and S. S. Kang Inhibition of Mitogen-Activated Kinase Kinase Kinase 3 Activity through Phosphorylation by the Serum- and Glucocorticoid-Induced Kinase 1 J. Biochem., January 1, 2003; 133(1): 103 - 108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Grey, Q. Chen, K. Callon, X. Xu, I. R. Reid, and J. Cornish The Phospholipids Sphingosine-1-Phosphate and Lysophosphatidic Acid Prevent Apoptosis in Osteoblastic Cells via a Signaling Pathway Involving Gi Proteins and Phosphatidylinositol-3 Kinase Endocrinology, December 1, 2002; 143(12): 4755 - 4763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R. Brickley, C. A. Mikosz, C. R. Hagan, and S. D. Conzen Ubiquitin Modification of Serum and Glucocorticoid-induced Protein Kinase-1 (SGK-1) J. Biol. Chem., November 1, 2002; 277(45): 43064 - 43070. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. A. Itani, K. Z. Liu, K. L. Cornish, J. R. Campbell, and C. P. Thomas Glucocorticoids stimulate human sgk1 gene expression by activation of a GRE in its 5'-flanking region Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E971 - E979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gomez-Angelats and J. A. Cidlowski Invited Review: Cell Volume Control and Signal Transduction in Apoptosis Toxicol Pathol, August 1, 2002; 30(5): 541 - 551. [Abstract] [PDF]
|
|
|
|
|
|
J. D. Stockand New ideas about aldosterone signaling in epithelia Am J Physiol Renal Physiol, April 1, 2002; 282(4): F559 - F576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Richards, S. C. Sharma, A. E. Falender, and Y. H. Lo Expression of FKHR, FKHRL1, and AFX Genes in the Rodent Ovary: Evidence for Regulation by IGF-I, Estrogen, and the Gonadotropins Mol. Endocrinol., March 1, 2002; 16(3): 580 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Lang and P. Cohen Regulation and Physiological Roles of Serum- and Glucocorticoid-Induced Protein Kinase Isoforms Sci. Signal., November 13, 2001; 2001(108): re17 - re17. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
