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Originally published In Press as doi:10.1074/jbc.M007462200 on January 24, 2001

J. Biol. Chem., Vol. 276, Issue 20, 16731-16738, May 18, 2001
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Nickel Resistance and Chromatin Condensation in Saccharomyces cerevisiae Expressing a Maize High Mobility Group I/Y Protein*

Céline Forzani, Clarisse Loulergue, Stéphane Lobréaux, Jean-François Briat, and Michel LebrunDagger

From Biochimie et Physiologie Moléculaire des Plantes, CNRS Unité Mixte de Recherche 5004, Université Montpellier 2, Institut National de la Recherche Agronomique, Ecole Nationale Supérieure d'Agronomie, 2 place Viala, F-34060 Montpellier Cedex 1, France

Expression of a maize cDNA encoding a high mobility group (HMG) I/Y protein enables growth of transformed yeast on a medium containing toxic nickel concentrations. No difference in the nickel content was measured between yeast cells expressing either the empty vector or the ZmHMG I/Y2 cDNA. The ZmHMG I/Y2 protein contains four AT hook motifs known to be involved in binding to the minor groove of AT-rich DNA regions. HMG I/Y proteins may act as architectural elements modifying chromatin structure. Indeed, a ZmHMG I/Y2-green fluorescent protein fusion protein was observed in yeast nuclei. Nickel toxicity has been suggested to occur through an epigenetic mechanism related to chromatin condensation and DNA methylation, leading to the silencing of neighboring genes. Therefore, the ZmHMG I/Y2 protein could prevent nickel toxicity by interfering with chromatin structure. Yeast cell growth in the presence of nickel and yeast cells expressing the ZmHMG I/Y2 cDNA increased telomeric URA3 gene silencing. Furthermore, ZmHMG I/Y2 restored a wild-type level of nickel sensitivity to the yeast Delta rpd3 mutant. Therefore, nickel resistance of yeast cells expressing the ZmHMG I/Y2 cDNA is likely achieved by chromatin structure modification, restricting nickel accessibility to DNA.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF291748.

Dagger To whom correspondence should be addressed. Tel.: 33-4-67-14-47-99; Fax: 33-4-67-14-36-37; E-mail: lebrun@arpb.univ-montp2.fr.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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