JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M010955200 on February 27, 2001

J. Biol. Chem., Vol. 276, Issue 20, 16904-16910, May 18, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
276/20/16904    most recent
M010955200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Le Lay, S.
Right arrow Articles by Dugail, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Le Lay, S.
Right arrow Articles by Dugail, I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Cholesterol, a Cell Size-dependent Signal That Regulates Glucose Metabolism and Gene Expression in Adipocytes*,

Soazig Le Lay, Stéphane KriefDagger , Céline Farnier, Isabelle LefrèreDagger , Xavier Le Liepvre, Raymond Bazin, Pascal Ferré, and Isabelle Dugail§

From the U465 INSERM, Centre de Recherches Biomédicales des Cordeliers (Université Paris 6), 15 Rue de l'Ecole de Médecine, 75270 Paris Cedex 06, France and the Dagger  Centre de Recherche Smithkline Beecham, 4 Rue du Chesnay Beauregard, 35260 Saint Grégoire, France

Enlarged fat cells exhibit modified metabolic capacities, which could be involved in the metabolic complications of obesity at the whole body level. We show here that sterol regulatory element-binding protein 2 (SREBP-2) and its target genes are induced in the adipose tissue of several models of rodent obesity, suggesting cholesterol imbalance in enlarged adipocytes. Within a particular fat pad, larger adipocytes have reduced membrane cholesterol concentrations compared with smaller fat cells, demonstrating that altered cholesterol distribution is characteristic of adipocyte hypertrophy per se. We show that treatment with methyl-beta -cyclodextrin, which mimics the membrane cholesterol reduction of hypertrophied adipocytes, induces insulin resistance. We also produced cholesterol depletion by mevastatin treatment, which activates SREBP-2 and its target genes. The analysis of 40 adipocyte genes showed that the response to cholesterol depletion implicated genes involved in cholesterol traffic (caveolin 2, scavenger receptor BI, and ATP binding cassette 1 genes) but also adipocyte-derived secretion products (tumor necrosis factor alpha , angiotensinogen, and interleukin-6) and proteins involved in energy metabolism (fatty acid synthase, GLUT 4, and UCP3). These data demonstrate that altering cholesterol balance profoundly modifies adipocyte metabolism in a way resembling that seen in hypertrophied fat cells from obese rodents or humans. This is the first evidence that intracellular cholesterol might serve as a link between fat cell size and adipocyte metabolic activity.

* This work was supported by contract FAIR 97-3011 from the European Community.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

The on-line version of this article (available at http://www.jbc.org) contains supplemental data.

§ To whom correspondence should be addressed. Tel.: 33 1 42 34 69 22/23/24; Fax: 33 1 40 51 85 86; E-mail: idugail@bhdc.jussieu.fr.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 DiabetesHome page
M. Hao, W. S. Head, S. C. Gunawardana, A. H. Hasty, and D. W. Piston
Direct Effect of Cholesterol on Insulin Secretion: A Novel Mechanism for Pancreatic {beta}-Cell Dysfunction
Diabetes, September 1, 2007; 56(9): 2328 - 2338.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Izem and R. E. Morton
Possible Role for Intracellular Cholesteryl Ester Transfer Protein in Adipocyte Lipid Metabolism and Storage
J. Biol. Chem., July 27, 2007; 282(30): 21856 - 21865.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. J. Nixon, A. Carter, J. Wegner, C. Ferguson, M. Floetenmeyer, J. Riches, B. Key, M. Westerfield, and R. G. Parton
Caveolin-1 is required for lateral line neuromast and notochord development
J. Cell Sci., July 1, 2007; 120(13): 2151 - 2161.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. Lu, C. M. Boustany-Kari, A. Daugherty, and L. A. Cassis
Angiotensin II increases adipose angiotensinogen expression
Am J Physiol Endocrinol Metab, May 1, 2007; 292(5): E1280 - E1287.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
Z. H. Huang, C. A. Reardon, and T. Mazzone
Endogenous ApoE Expression Modulates Adipocyte Triglyceride Content and Turnover
Diabetes, December 1, 2006; 55(12): 3394 - 3402.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. Jernas, J. Palming, K. Sjoholm, E. Jennische, P.-A. Svensson, B. G. Gabrielsson, M. Levin, A. Sjogren, M. Rudemo, T. C. Lystig, et al.
Separation of human adipocytes by size: hypertrophic fat cells display distinct gene expression
FASEB J, July 1, 2006; 20(9): 1540 - 1542.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
X. Lu, F. Kambe, X. Cao, T. Yoshida, S. Ohmori, K. Murakami, T. Kaji, T. Ishii, D. Zadworny, and H. Seo
DHCR24-Knockout Embryonic Fibroblasts Are Susceptible to Serum Withdrawal-Induced Apoptosis Because of Dysfunction of Caveolae and Insulin-Akt-Bad Signaling
Endocrinology, June 1, 2006; 147(6): 3123 - 3132.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A.-L. Tondu, C. Robichon, L. Yvan-Charvet, N. Donne, X. Le Liepvre, E. Hajduch, P. Ferre, I. Dugail, and G. Dagher
Insulin and Angiotensin II Induce the Translocation of Scavenger Receptor Class B, Type I from Intracellular Sites to the Plasma Membrane of Adipocytes
J. Biol. Chem., September 30, 2005; 280(39): 33536 - 33540.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Venugopal, K. Hanashiro, Z.-Z. Yang, and Y. Nagamine
Identification and modulation of a caveolae-dependent signal pathway that regulates plasminogen activator inhibitor-1 in insulin-resistant adipocytes
PNAS, December 7, 2004; 101(49): 17120 - 17125.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Yue, N. Rasouli, G. Ranganathan, P. A. Kern, and T. Mazzone
Divergent Effects of Peroxisome Proliferator-activated Receptor {gamma} Agonists and Tumor Necrosis Factor {alpha} on Adipocyte ApoE Expression
J. Biol. Chem., November 12, 2004; 279(46): 47626 - 47632.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Morel, S. Fouquet, D. Chateau, L. Yvernault, Y. Frobert, M. Pincon-Raymond, J. Chambaz, T. Pillot, and M. Rousset
The Cellular Prion Protein PrPc Is Expressed in Human Enterocytes in Cell-Cell Junctional Domains
J. Biol. Chem., January 9, 2004; 279(2): 1499 - 1505.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
G. Dagher, N. Donne, C. Klein, P. Ferre, and I. Dugail
HDL-mediated cholesterol uptake and targeting to lipid droplets in adipocytes
J. Lipid Res., October 1, 2003; 44(10): 1811 - 1820.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
S. Le Lay, C. Robichon, X. Le Liepvre, G. Dagher, P. Ferre, and I. Dugail
Regulation of ABCA1 expression and cholesterol efflux during adipose differentiation of 3T3-L1 cells
J. Lipid Res., August 1, 2003; 44(8): 1499 - 1507.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
T. Chiba, T. Nakazawa, K. Yui, E. Kaneko, and K. Shimokado
VLDL Induces Adipocyte Differentiation in ApoE-Dependent Manner
Arterioscler. Thromb. Vasc. Biol., August 1, 2003; 23(8): 1423 - 1429.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
L. J. Pike
Lipid rafts: bringing order to chaos
J. Lipid Res., April 1, 2003; 44(4): 655 - 667.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. PLAT and R. P. MENSINK
Increased intestinal ABCA1 expression contributes to the decrease in cholesterol absorption after plant stanol consumption
FASEB J, August 1, 2002; 16(10): 1248 - 1253.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J.A. Paniagua, J. Lopez-Miranda, A. Escribano, F.J. Berral, C. Marin, D. Bravo, E. Paz-Rojas, P. Gomez, M. Barcos, J.A. Moreno, et al.
Cerivastatin Improves Insulin Sensitivity and Insulin Secretion in Early-State Obese Type 2 Diabetes
Diabetes, August 1, 2002; 51(8): 2596 - 2603.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
I. Guillet-Deniau, V. Mieulet, S. Le Lay, Y. Achouri, D. Carre, J. Girard, F. Foufelle, and P. Ferre
Sterol Regulatory Element Binding Protein-1c Expression and Action in Rat Muscles: Insulin-Like Effects on the Control of Glycolytic and Lipogenic Enzymes and UCP3 Gene Expression
Diabetes, June 1, 2002; 51(6): 1722 - 1728.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Y. Zhang, K.-Y. Guo, P. A. Diaz, M. Heo, and R. L. Leibel
Determinants of leptin gene expression in fat depots of lean mice
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2002; 282(1): R226 - R234.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.