JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M008048200 on February 26, 2001

J. Biol. Chem., Vol. 276, Issue 20, 16952-16959, May 18, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/20/16952    most recent
M008048200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Englund, G. D.
Right arrow Articles by Du, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Englund, G. D.
Right arrow Articles by Du, X.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Regulation of von Willebrand Factor Binding to the Platelet Glycoprotein Ib-IX by a Membrane Skeleton-dependent Inside-out Signal*

Graham D. EnglundDagger §, Richard J. BodnarDagger §, Zhenyu LiDagger , Zaverio M. Ruggeri, and Xiaoping DuDagger ||

From the Dagger  Department of Pharmacology, University of Illinois, College of Medicine, Chicago, Illinois 60612 and the  Departments of Molecular and Experimental Medicine and of Vascular Biology, The Scripps Research Institute, La Jolla, California 92037

The platelet receptor for von Willebrand factor (vWF), glycoprotein Ib-IX (GPIb-IX), mediates initial platelet adhesion and activation. We show here that the receptor function of GPIb-IX is regulated intracellularly via its link to the filamin-associated membrane skeleton. Deletion of the filamin binding site in GPIbalpha markedly enhances ristocetin- (or botrocetin)-induced vWF binding and allows GPIb-IX-expressing cells to adhere to immobilized vWF under both static and flow conditions. Cytochalasin D (CD) that depolymerizes actin also enhances vWF binding to wild type GPIb-IX. Thus, vWF binding to GPIb-IX is negatively regulated by the filamin-associated membrane skeleton. In contrast to native vWF, binding of the isolated recombinant vWF A1 domain to wild type and filamin binding-deficient mutants of GPIb-IX is comparable, suggesting that the membrane skeleton-associated GPIb-IX is in a state that prevents access to the A1 domain in macromolecular vWF. In platelets, there is a balance of membrane skeleton-associated and free forms of GPIb-IX. Treatment of platelets with CD increases the free form and enhances vWF binding. CD also reverses the inhibitory effects of prostaglandin E1 on vWF binding to GPIb-IX. Thus, GPIb-IX-dependent platelet adhesion is doubly controlled by vWF conformation and a membrane skeleton-dependent inside-out signal.

* This work was supported in part by National Institutes of Health Grants HL52547 and HL62350 (to X. D.) and HL31950, HL42846, and HL48728 (to Z. M. R.) and by a grant-in-aid from the American Heart Association.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ Both authors contributed equally to this work.

|| An Established Investigator of the American Heart Association. To whom correspondence should be addressed: Dept. of Pharmacology, University of Illinois, College of Medicine, 835 S. Wolcott Ave., Chicago, IL 60612. Tel.: 312-355-0237; Fax: 312-996-1225; E-mail: xdu@uic.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 BloodHome page
H. Yin, A. Stojanovic, N. Hay, and X. Du
The role of Akt in the signaling pathway of the glycoprotein Ib-IX induced platelet activation
Blood, January 15, 2008; 111(2): 658 - 665.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. J. Clemetson
How complex is the platelet GP Ib complex?
Blood, January 15, 2007; 109(2): 393 - 394.
[Full Text] [PDF]

Home page
 BloodHome page
S.-Z. Luo, X. Mo, V. Afshar-Kharghan, S. Srinivasan, J. A. Lopez, and R. Li
Glycoprotein Ib{alpha} forms disulfide bonds with 2 glycoprotein Ib{beta} subunits in the resting platelet
Blood, January 15, 2007; 109(2): 603 - 609.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Mo, N. Lu, A. Padilla, J. A. Lopez, and R. Li
The Transmembrane Domain of Glycoprotein Ibbeta Is Critical to Efficient Expression of Glycoprotein Ib-IX Complex in the Plasma Membrane
J. Biol. Chem., August 11, 2006; 281(32): 23050 - 23059.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. J. Reininger, H. F. G. Heijnen, H. Schumann, H. M. Specht, W. Schramm, and Z. M. Ruggeri
Mechanism of platelet adhesion to von Willebrand factor and microparticle formation under high shear stress
Blood, May 1, 2006; 107(9): 3537 - 3545.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
C. Weeterings, J. Adelmeijer, T. Myles, P. G. de Groot, and T. Lisman
Glycoprotein Ib{alpha}-Mediated Platelet Adhesion and Aggregation to Immobilized Thrombin Under Conditions of Flow
Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 670 - 675.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. Dai, R. Bodnar, M. C. Berndt, and X. Du
A critical role for 14-3-3{zeta} protein in regulating the VWF binding function of platelet glycoprotein Ib-IX and its therapeutic implications
Blood, September 15, 2005; 106(6): 1975 - 1981.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
R. Guerrini, D. Mei, S. Sisodiya, F. Sicca, B. Harding, Y. Takahashi, T. Dorn, A. Yoshida, J. Campistol, G. Kramer, et al.
Germline and mosaic mutations of FLN1 in men with periventricular heterotopia
Neurology, July 13, 2004; 63(1): 51 - 56.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. L. Cranmer and S. P. Jackson
GPIb, filamin, and platelet activation: a view from within
Blood, September 15, 2003; 102(6): 1937 - 1937.
[Full Text] [PDF]

Home page
 BloodHome page
S. Feng, J. C. Resendiz, X. Lu, and M. H. Kroll
Filamin A binding to the cytoplasmic tail of glycoprotein Ib{alpha} regulates von Willebrand factor-induced platelet activation
Blood, September 15, 2003; 102(6): 2122 - 2129.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. M. Dyson, A. D. Munday, A. M. Kong, R. D. Huysmans, M. Matzaris, M. J. Layton, H. H. Nandurkar, M. C. Berndt, and C. A. Mitchell
SHIP-2 forms a tetrameric complex with filamin, actin, and GPIb-IX-V: localization of SHIP-2 to the activated platelet actin cytoskeleton
Blood, August 1, 2003; 102(3): 940 - 948.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
X. Xi, R. J. Bodnar, Z. Li, S. C.-T. Lam, and X. Du
Critical roles for the COOH-terminal NITY and RGT sequences of the integrin {beta}3 cytoplasmic domain in inside-out and outside-in signaling
J. Cell Biol., July 21, 2003; 162(2): 329 - 339.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Z. Li, J. Ajdic, M. Eigenthaler, and X. Du
A predominant role for cAMP-dependent protein kinase in the cGMP-induced phosphorylation of vasodilator-stimulated phosphoprotein and platelet inhibition in humans
Blood, June 1, 2003; 101(11): 4423 - 4429.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Bodnar, X. Xi, Z. Li, M. C. Berndt, and X. Du
Regulation of Glycoprotein Ib-IX-von Willebrand Factor Interaction by cAMP-dependent Protein Kinase-mediated Phosphorylation at Ser 166 of Glycoprotein Ibbeta
J. Biol. Chem., November 27, 2002; 277(49): 47080 - 47087.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Navdaev and K. J. Clemetson
Glycoprotein Ib Cross-linking/Ligation on Echicetin-coated Surfaces or Echicetin-IgMkappa in Stirred Suspension Activates Platelets by Cytoskeleton Modulated Calcium Release
J. Biol. Chem., November 22, 2002; 277(48): 45928 - 45934.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Kanaji, S. Russell, and J. Ware
Amelioration of the macrothrombocytopenia associated with the murine Bernard-Soulier syndrome
Blood, August 28, 2002; 100(6): 2102 - 2107.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kasirer-Friede, J. Ware, L. Leng, P. Marchese, Z. M. Ruggeri, and S. J. Shattil
Lateral Clustering of Platelet GP Ib-IX Complexes Leads to Up-regulation of the Adhesive Function of Integrin alpha IIbbeta 3
J. Biol. Chem., March 29, 2002; 277(14): 11949 - 11956.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Williamson, I. Pikovski, S. L. Cranmer, P. Mangin, N. Mistry, T. Domagala, S. Chehab, F. Lanza, H. H. Salem, and S. P. Jackson
Interaction between Platelet Glycoprotein Ibalpha and Filamin-1 Is Essential for Glycoprotein Ib/IX Receptor Anchorage at High Shear
J. Biol. Chem., January 11, 2002; 277(3): 2151 - 2159.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.