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Originally published In Press as doi:10.1074/jbc.M100623200 on March 6, 2001

J. Biol. Chem., Vol. 276, Issue 20, 17022-17029, May 18, 2001
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Excision of 3' Termini by the Trex1 and TREX2 3'right-arrow 5' Exonucleases
CHARACTERIZATION OF THE RECOMBINANT PROTEINS*

Dan J. Mazur and Fred W. PerrinoDagger

From the Department of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157

The excision of nucleotides from DNA 3' termini is an important step in DNA replication, repair, and recombination pathways to generate correctly base paired termini for subsequent processing. The mammalian TREX1 and TREX2 proteins contain potent 3'right-arrow5' exonucleases capable of functioning in this capacity. To study the activities of these exonucleases we have developed strategies to express and purify the recombinant mouse Trex1 and human TREX2 proteins in Escherichia coli in quantities sufficient for biochemical characterization. The Trex1 and TREX2 proteins are homodimers that exhibit robust 3' excision activities with very similar preferred reaction conditions and preferences for specific DNA substrates. In a steady-state kinetic analysis, oligonucleotide substrates were used to measure 3' nucleotide excision by Trex1 and TREX2. The Michaelis constants derived from these data indicate similar apparent kcat values of 22 s-1 for Trex1 and 16 s-1 for TREX2 using single-stranded oligonucleotides. The apparent KM values of 19 nM for Trex1 and 190 nM for TREX2 suggest relatively high affinities for DNA for both Trex1 and TREX2. An exonuclease competition assay was designed using heparin as a nonsubstrate inhibitor with a series of partial duplex DNAs to delineate the substrate structure preferences for 3' nucleotide excision by Trex1 and TREX2. The catalytic properties of the TREX proteins suggest roles for these enzymes in the 3' end-trimming processes necessary for producing correctly base paired 3' termini.

* This work was supported by National Institutes of Health Grants CA75350 and CA12197.The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Dept. of Biochemistry, Wake Forest University School of Medicine, Winston-Salem, NC 27157. Tel.: 336-716-4349; Fax: 336-716-7200; E-mail: fperrino@wfubmc.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.


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