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J. Biol. Chem., Vol. 276, Issue 20, 17069-17075, May 18, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/20/17069    most recent M009064200v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tye, A. J. Articles by Lim, B.-L. Search for Related Content PubMed PubMed Citation Articles by Tye, A. J. Articles by Lim, B.-L. Social Bookmarking                      What's this?

The Human gC1qR/p32 Gene, C1qBP
GENOMIC ORGANIZATION AND PROMOTER ANALYSIS^*

Angela J. Tye, Berhane Ghebrehiwet^§, Ning Guo^¶, Kedarnath N. Sastry^¶, Billy K. C. Chow, Ellinor I. B. Peerschke, and Boon-Leong Lim^***

 Department of Zoology, University of Hong Kong, Pokfulam, Hong Kong, China, ^§ Department of Medicine, State University of New York, Stony Brook, New York, 11794-8161, ^¶ Department of Pathology, Weill College of Medicine, Cornell University, New York, New York 10021, and  Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, Massachusetts 02118

gC1qR is an ubiquitously expressed cell protein that interacts with the globular heads of C1q (gC1q) and many other ligands. In this study, the 7.8-kilobase pair (kb) human gC1qR/p32 (C1qBP) gene was cloned and found to consist of 6 exons and 5 introns. Analysis of a 1.3-kb DNA fragment at the 5'-flanking region of this gene revealed the presence of multiple TATA, CCAAT, and Sp1 binding sites. Luciferase reporter assays performed in different human cell lines demonstrated that the reporter gene was ubiquitously driven by this 1.3-kb fragment. Subsequent 5' and 3' deletion of this fragment confined promoter elements to within 400 base pairs (bp) upstream of the translational start site. Because the removal of the 8-bp consensus TATATATA at 399 to 406 and CCAAT at 410 to 414 did not significantly affect the transcription efficiency of the promoter, GC-rich sequences between this TATA box and the translation start site may be very important for the promoter activity of the C1qBP gene. One of seven GC-rich sequences in this region binds specifically to PANC-1 nuclear extracts, and the transcription factor Sp1 was shown to bind to this GC-rich sequence by the supershift assay. Primer extension analysis mapped three major transcription start regions. The farthest transcription start site is 49 bp upstream of the ATG translation initiation codon and is in close proximity of the specific SP1 binding site.

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