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J. Biol. Chem., Vol. 276, Issue 20, 17117-17124, May 18, 2001
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From the Divisions of The general transcription factor IID consists of
the TATA-binding protein (TBP) and multiple TBP-associated factors
(TAFs). Here we report the isolation of two related TAF genes from the fission yeast Schizosaccharomyces pombe as multicopy
suppressors of a temperature-sensitive mutation in the
ubiquitin-conjugating enzyme gene ubcP4+.
The ubcP4ts mutation causes cell cycle arrest in
mitosis, probably due to defects in ubiquitination mediated by the
anaphase-promoting complex/cyclosome. One multicopy suppressor
is the previously reported gene taf72+,
whereas the other is a previously unidentified gene named
taf73+. We show that the
taf73+ gene, like
taf72+, is essential for cell viability. The
taf72+ and taf73+ genes
encode proteins homologous to WD repeat-containing TAFs such as human
TAF100, Drosophila TAF80/85, and Saccharomyces
cerevisiae TAF90. We demonstrate that TAF72 and TAF73 proteins
are present in the same complex with TBP and other TAFs and that TAF72,
but not TAF73, is associated with the putative histone acetylase Gcn5. We also show that overexpression of TAF72 or TAF73 suppresses the cell
cycle arrest in mitosis caused by a mutation in the anaphase-promoting complex/cyclosome subunit gene cut9+. These
results suggest that TAF72 and TAF73 may regulate the expression of
genes involved in ubiquitin-dependent proteolysis during
mitosis. Our study thus provides evidence for a possible role of WD
repeat-containing TAFs in the expression of genes involved in
progression through the M phase of the cell cycle.
The nucleotide sequence reported in this paper has been submitted
to the DDBJ/GenBankTM/EBI Data Bank under
accession number AB039954.
Two WD Repeat-containing TATA-binding Protein-associated
Factors in Fission Yeast That Suppress Defects in the
Anaphase-promoting Complex*
§,
Molecular Genetics and
¶ Mutagenesis, Department of Molecular Genetics, National
Institute of Genetics, Mishima, Shizuoka 411-8540, Japan
*
This work was supported by grants from the Ministry of
Education, Science, Sports, and Culture of Japan and the Japan Science and Technology Corp.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
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