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Originally published In Press as doi:10.1074/jbc.M011569200 on February 23, 2001

J. Biol. Chem., Vol. 276, Issue 20, 17474-17478, May 18, 2001
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Molecular Cloning and Characterization of a Novel Mammalian Endo-apyrase (LALP1)*

Jing-Da ShiDagger §, Thomas Kukar§||, Cong-Yi WangDagger §, Quan-Zhen LiDagger , Pedro E. CruzDagger , Abdoreza Davoodi-SemiromiDagger , Ping YangDagger , Yunrong Gu, Wei Lian, Donghai H. Wu, and Jin-Xiong SheDagger **

From the Dagger  Department of Pathology, Immunology and Laboratory Medicine, Center for Mammalian Genetics and Diabetes Center of Excellence, College of Medicine, and  Department of Medicinal Chemistry, College of Pharmacy and The McKnight Brain Institute, University of Florida, Gainesville, Florida 32610

Here we describe the cloning, localization, and characterization of a novel mammalian endo-apyrase (LALP1) in human and mouse. The predicted human LALP1 gene encodes a 604-amino acid protein, whereas the mouse Lalp1 gene encodes a 606-amino acid protein. The human and mouse genes have 88% amino acid sequence identity. These genes share considerable homologies with hLALP70, a recently discovered mammalian lysosomal endo-apyrase. The human LALP1 gene resides on chromosome 10q23-q24 and contains 12 exons and 11 introns covering a genomic region of ~46 kilobase pairs. The subcellular localization and enzymatic activity of LALP1 indicated that LALP1 is indeed an endo-apyrase with substrate preference for nucleoside triphosphates UTP, GTP, and CTP.

* This research was supported in part by National Institutes of Health Grants 1R01DK53266-01 (to J.-X. S) and AG015688 (to D. H. W).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

§ These authors contributed equally to this work.

|| Supported by a University of Florida Alumni Fellowship.

** To whom correspondence should be addressed: Dept. of Pathology, Immunology and Laboratory Medicine, Box 100275, University of Florida, Gainesville, FL 32610. Tel.: 352-392-0677; Fax: 352-392-3053; E-mail: she@ufl.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
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