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J. Biol. Chem., Vol. 276, Issue 20, 17515-17523, May 18, 2001
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From the Institute of Molecular Agrobiology, The National
University of Singapore, 1 Research Link, Singapore 117604
One missing link in the coronavirus assembly is
the physical interaction between two crucial structural proteins, the
membrane (M) and envelope (E) proteins. In this study, we demonstrate
that the coronavirus infectious bronchitis virus E can
physically interact, via a putative peripheral domain, with M. Deletion
of this domain resulted in a drastic reduction in the incorporation of
M into virus-like particles. Immunofluorescent staining of cells
coexpressing M and E supports that E interacts with M and relocates M
to the same subcellular compartments that E resides in. E was retained in the pre-Golgi membranes, prior to being translocated to the Golgi
apparatus and the secretory vesicles; M was observed to exhibit similar
localization and translocation profiles as E when coexpressed with E. Deletion studies identified the C-terminal 6-residue RDKLYS as the
endoplasmic reticulum retention signal of E, and site-directed
mutagenesis of the
The Missing Link in Coronavirus Assembly
RETENTION OF THE AVIAN CORONAVIRUS INFECTIOUS BRONCHITIS VIRUS
ENVELOPE PROTEIN IN THE PRE-GOLGI COMPARTMENTS AND PHYSICAL INTERACTION
BETWEEN THE ENVELOPE AND MEMBRANE PROTEINS*
4 lysine residue to glutamine resulted in the
accumulation of E in the Golgi apparatus. The third domain of E that
plays a crucial role in virus budding is a putative transmembrane
domain present at the N-terminal region, because deletion of the domain
resulted in a free distribution of the mutant protein and in
dysfunctional viral assembly.
*
This work is supported by a grant from the National Science
and Technology Board of Singapore.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Tel.:
65-8727468; Fax: 65-8727007; E-mail: liudx@ima.org.sg.
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