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J. Biol. Chem., Vol. 276, Issue 20, 17597-17602, May 18, 2001

This Article Full Text Full Text (PDF) All Versions of this Article: 276/20/17597    most recent M011769200v1 Submit a Letter to Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bolduc, L. Articles by Chabot, B. Search for Related Content PubMed PubMed Citation Articles by Bolduc, L. Articles by Chabot, B. Social Bookmarking                      What's this?

Dimethyl Sulfoxide Affects the Selection of Splice Sites^*

Lucie Bolduc, Benoit Labrecque, Mélanie Cordeau, Marco Blanchette^§, and Benoit Chabot^¶

From the Département de Microbiologie et d'Infectiologie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4

Depending on the cell lines and cell types, dimethyl sulfoxide (Me₂SO) can induce or block cell differentiation and apoptosis. Although Me₂SO treatment alters many levels of gene expression, the molecular processes that are directly affected by Me₂SO have not been clearly identified. Here, we report that Me₂SO affects splice site selection on model pre-mRNAs incubated in a nuclear extract prepared from HeLa cells. A shift toward the proximal pair of splice sites was observed on pre-mRNAs carrying competing 5'-splice sites or competing 3'-splice sites. Because the activity of recombinant hnRNP A1 protein was similar when added to extracts containing or lacking Me₂SO, the activity of endogenous A1 proteins is probably not affected by Me₂SO. Notably, in a manner reminiscent of SR proteins, Me₂SO activated splicing in a HeLa S100 extract. Moreover, the activity of recombinant SR proteins in splice site selection in vitro was improved by Me₂SO. Polar solvents like DMF and formamide similarly modulated splice site selection in vitro but formamide did not activate a HeLa S100 extract. We propose that Me₂SO improves ionic interactions between splicing factors that contain RS-domains. The direct impact of Me₂SO on alternative splicing may explain, at least in part, the different and sometimes opposite effects of Me₂SO on cell differentiation and apoptosis.

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