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J. Biol. Chem., Vol. 276, Issue 21, 17712-17717, May 25, 2001
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Laboratory of Cell Biology, Howard Hughes Medical Institute,
Rockefeller University, New York, New York 10021
In Saccharomyces cerevisiae, Spo12p
is involved in mitosis and is essential for meiosis. We found that
Spo12p is imported into the nucleus by the karyopherin Kap121p. A
complex containing Spo12p and Kap121p was isolated from cytosol and was
also reconstituted with recombinant proteins, indicating that this
interaction is direct. Spo12p was mislocalized to the cytosol in
pse1-1, a temperature-sensitive strain harboring a
mutation of Kap121p, at the permissive temperature, confirming an
essential role for Kap121p in Spo12p import. Spo12p was also
mislocalized in a pse1-1/pse1-1 homozygous strain,
suggesting it is imported via the same pathway in diploid cells.
Furthermore, we found that pse1-1/pse1-1 shows a
sporulation defect similar to that of
spo12
Nuclear Import of Spo12p, a Protein Essential for Meiosis*
and
/spo12. In addition, we
have characterized the Spo12p nuclear localization signal, mapped it to
residues 76-130, and identified residues within this region that are
important for nuclear localization signal function.
*
The costs of publication of this
article were defrayed in part by the
payment of page charges. The article
must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
Recipient of a fellowship from PRAXIS XXI, Portugal.
§
To whom correspondence should be addressed. Tel.: 212-327-8096;
Fax: 212-327-7880; E-mail:
blobel@rockvax.rockefeller.edu.
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