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J. Biol. Chem., Vol. 276, Issue 21, 17878-17886, May 25, 2001
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From the The DNA architectural proteins FIS and CRP
are global regulators of transcription in Escherichia coli
involved in the adjustment of cellular metabolism to varying growth
conditions. We have previously demonstrated that FIS modulates the
expression of the crp gene by functioning as its
transcriptional repressor. Here we show that in turn, CRP is required
to maintain the growth phase pattern of fis expression. We
demonstrate the existence of a divergent promoter in the
fis regulatory region, which reduces transcription of the
fis promoter. In the absence of FIS, CRP activates
fis transcription, thereby displacing the polymerase from
the divergent promoter, whereas together FIS and CRP synergistically
repress fis gene expression. These results provide evidence
for a direct cross-talk between global regulators of cellular
transcription during the growth phase. This cross-talk is manifested in
alternate formation of functional nucleoprotein complexes exerting
either activating or repressing effects on transcription.
CRP Modulates fis Transcription by Alternate
Formation of Activating and Repressing Nucleoprotein Complexes*
,§,
Institut für Genetik und
Mikrobiologie, Ludwig-Maximilians-Univesitaet,
Maria-Ward-Strasse 1a, 80638 München, Germany and the
¶ Medical Research Council Laboratory of Molecular Biology,
Hills Road, Cambridge CB2 2QH, United Kingdom
*
This work was supported by a grant from the
Département des Sciences de la Vie du CNRS (to W. N.) and by
the Deutsche Forschungsgemeinschaft through Grant SFB 190.The costs of publication of this
article were defrayed in part by the
payment of page charges. The article must therefore be hereby marked
"advertisement" in
accordance with 18 U.S.C. Section
1734 solely to indicate this fact.
To whom correspondence should be addressed. Present address:
Max-Planck-Institute for Terrestrial Microbiology,
Karl-von-Frisch-Strasse, D-35043 Marburg, Germany. Tel.:
49-89-2180-6161; Fax: 49-89-2180-6160; E-mail:
Georgi.Muskheli@lrz.uni-muenchen.de.
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