JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/jbc.M100156200 on March 8, 2001

J. Biol. Chem., Vol. 276, Issue 21, 17920-17931, May 25, 2001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
276/21/17920    most recent
M100156200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Le Naour, F.
Right arrow Articles by Beretta, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Le Naour, F.
Right arrow Articles by Beretta, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Profiling Changes in Gene Expression during Differentiation and Maturation of Monocyte-derived Dendritic Cells Using Both Oligonucleotide Microarrays and Proteomics*

François Le NaourDagger , Lyndon Hohenkirk§, Annabelle GrolleauDagger , David E. Misek, Pascal Lescure, James D. Geiger§, Samir Hanash, and Laura BerettaDagger ||

From the Dagger  Department of Microbiology and Immunology, the  Department of Pediatrics, and the § Department of Surgery, University of Michigan, Ann Arbor, Michigan 48109-0666

Dendritic cells (DCs) are antigen-presenting cells that play a major role in initiating primary immune responses. We have utilized two independent approaches, DNA microarrays and proteomics, to analyze the expression profile of human CD14+ blood monocytes and their derived DCs. Analysis of gene expression changes at the RNA level using oligonucleotide microarrays complementary to 6300 human genes showed that ~40% of the genes were expressed in DCs. A total of 255 genes (4%) were found to be regulated during DC differentiation or maturation. Most of these genes were not previously associated with DCs and included genes encoding secreted proteins as well as genes involved in cell adhesion, signaling, and lipid metabolism. Protein analysis of the same cell populations was done using two-dimensional gel electrophoresis. A total of 900 distinct protein spots were included, and 4% of them exhibited quantitative changes during DC differentiation and maturation. Differentially expressed proteins were identified by mass spectrometry and found to represent proteins with Ca2+ binding, fatty acid binding, or chaperone activities as well as proteins involved in cell motility. In addition, proteomic analysis provided an assessment of post-translational modifications. The chaperone protein, calreticulin, was found to undergo cleavage, yielding a novel form. The combined oligonucleotide microarray and proteomic approaches have uncovered novel genes associated with DC differentiation and maturation and has allowed analysis of post-translational modifications of specific proteins as part of these processes.

* The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

|| To whom correspondence should be addressed: Dept. of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109-0666. Tel.: 734-615-5964; Fax: 734-615-6150; E-mail: berettal@umich.edu.

Copyright © 2001 by The American Society for Biochemistry and Molecular Biology, Inc.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol Cancer ResHome page
A. Ricciardi, A. R. Elia, P. Cappello, M. Puppo, C. Vanni, P. Fardin, A. Eva, D. Munroe, X. Wu, M. Giovarelli, et al.
Transcriptome of Hypoxic Immature Dendritic Cells: Modulation of Chemokine/Receptor Expression
Mol. Cancer Res., February 1, 2008; 6(2): 175 - 185.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Illario, M. L. Giardino-Torchia, U. Sankar, T. J. Ribar, M. Galgani, L. Vitiello, A. M. Masci, F. R. Bertani, E. Ciaglia, D. Astone, et al.
Calmodulin-dependent kinase IV links Toll-like receptor 4 signaling with survival pathway of activated dendritic cells
Blood, January 15, 2008; 111(2): 723 - 731.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
I. Szatmari, D. Torocsik, M. Agostini, T. Nagy, M. Gurnell, E. Barta, K. Chatterjee, and L. Nagy
PPAR{gamma} regulates the function of human dendritic cells primarily by altering lipid metabolism
Blood, November 1, 2007; 110(9): 3271 - 3280.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
A. Lehtonen, H. Ahlfors, V. Veckman, M. Miettinen, R. Lahesmaa, and I. Julkunen
Gene expression profiling during differentiation of human monocytes to macrophages or dendritic cells
J. Leukoc. Biol., September 1, 2007; 82(3): 710 - 720.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
I. K. Demedts, K. R. Bracke, G. Van Pottelberge, D. Testelmans, G. M. Verleden, F. E. Vermassen, G. F. Joos, and G. G. Brusselle
Accumulation of Dendritic Cells and Increased CCL20 Levels in the Airways of Patients with Chronic Obstructive Pulmonary Disease
Am. J. Respir. Crit. Care Med., May 15, 2007; 175(10): 998 - 1005.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
N. Radoja, A. Gazel, T. Banno, S. Yano, and M. Blumenberg
Transcriptional profiling of epidermal differentiation
Physiol Genomics, January 12, 2007; 27(1): 65 - 78.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. S. Rolph, T. R. Young, B. O. V. Shum, C. Z. Gorgun, C. Schmitz-Peiffer, I. A. Ramshaw, G. S. Hotamisligil, and C. R. Mackay
Regulation of Dendritic Cell Function and T Cell Priming by the Fatty Acid-Binding Protein aP2
J. Immunol., December 1, 2006; 177(11): 7794 - 7801.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. A. Fulcher, S. T. Hashimi, E. L. Levroney, M. Pang, K. B. Gurney, L. G. Baum, and B. Lee
Galectin-1-Matured Human Monocyte-Derived Dendritic Cells Have Enhanced Migration through Extracellular Matrix
J. Immunol., July 1, 2006; 177(1): 216 - 226.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. Luft, E. Rodionova, E. Maraskovsky, M. Kirsch, M. Hess, C. Buchholtz, M. Goerner, M. Schnurr, R. Skoda, and A. D. Ho
Adaptive functional differentiation of dendritic cells: integrating the network of extra- and intracellular signals
Blood, June 15, 2006; 107(12): 4763 - 4769.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
S. Talwar, P. J. Munson, J. Barb, C. Fiuza, A. P. Cintron, C. Logun, M. Tropea, S. Khan, D. Reda, J. H. Shelhamer, et al.
Gene expression profiles of peripheral blood leukocytes after endotoxin challenge in humans
Physiol Genomics, April 13, 2006; 25(2): 203 - 215.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. Rivollier, L. Perrin-Cocon, S. Luche, H. Diemer, J.-M. Strub, D. Hanau, A. van Dorsselaer, V. Lotteau, C. Rabourdin-Combe, T. Rabilloud, et al.
High Expression of Antioxidant Proteins in Dendritic Cells: Possible Implications in Atherosclerosis
Mol. Cell. Proteomics, April 1, 2006; 5(4): 726 - 736.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K. Murata, T. Hayashibara, K. Sugahara, A. Uemura, T. Yamaguchi, H. Harasawa, H. Hasegawa, K. Tsuruda, T. Okazaki, T. Koji, et al.
A Novel Alternative Splicing Isoform of Human T-Cell Leukemia Virus Type 1 bZIP Factor (HBZ-SI) Targets Distinct Subnuclear Localization
J. Virol., March 1, 2006; 80(5): 2495 - 2505.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
I K Demedts, A Morel-Montero, S Lebecque, Y Pacheco, D Cataldo, G F Joos, R A Pauwels, and G G Brusselle
Elevated MMP-12 protein levels in induced sputum from patients with COPD
Thorax, March 1, 2006; 61(3): 196 - 201.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
C. M. Meyers, M. Geanacopoulos, L. B. Holzman, and D. J. Salant
Glomerular Disease Workshop
J. Am. Soc. Nephrol., December 1, 2005; 16(12): 3472 - 3476.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
C. A. Ryan, G. F. Gerberick, L. A. Gildea, B. C. Hulette, C. J. Betts, M. Cumberbatch, R. J. Dearman, and I. Kimber
Interactions of Contact Allergens with Dendritic Cells: Opportunities and Challenges for the Development of Novel Approaches to Hazard Assessment
Toxicol. Sci., November 1, 2005; 88(1): 4 - 11.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
D. McIlroy, S. Tanguy-Royer, N. Le Meur, I. Guisle, P.-J. Royer, J. Leger, K. Meflah, and M. Gregoire
Profiling dendritic cell maturation with dedicated microarrays
J. Leukoc. Biol., September 1, 2005; 78(3): 794 - 803.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K. Vermaelen and R. Pauwels
Pulmonary Dendritic Cells
Am. J. Respir. Crit. Care Med., September 1, 2005; 172(5): 530 - 551.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
D. L. Corcoran, E. Feingold, J. Dominick, M. Wright, J. Harnaha, M. Trucco, N. Giannoukakis, and P. V. Benos
Footer: A quantitative comparative genomics method for efficient recognition of cis-regulatory elements
Genome Res., June 1, 2005; 15(6): 840 - 847.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
T. Tamura, P. Tailor, K. Yamaoka, H. J. Kong, H. Tsujimura, J. J. O'Shea, H. Singh, and K. Ozato
IFN Regulatory Factor-4 and -8 Govern Dendritic Cell Subset Development and Their Functional Diversity
J. Immunol., March 1, 2005; 174(5): 2573 - 2581.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. J. Nauta, G. Castellano, W. Xu, A. M. Woltman, M. C. Borrias, M. R. Daha, C. van Kooten, and A. Roos
Opsonization with C1q and Mannose-Binding Lectin Targets Apoptotic Cells to Dendritic Cells
J. Immunol., September 1, 2004; 173(5): 3044 - 3050.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S.-W. Qiao, E. Bergseng, O. Molberg, J. Xia, B. Fleckenstein, C. Khosla, and L. M. Sollid
Antigen Presentation to Celiac Lesion-Derived T Cells of a 33-Mer Gliadin Peptide Naturally Formed by Gastrointestinal Digestion
J. Immunol., August 1, 2004; 173(3): 1757 - 1762.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
G. Castellano, A. M. Woltman, A. J. Nauta, A. Roos, L. A. Trouw, M. A. Seelen, F. P. Schena, M. R. Daha, and C. van Kooten
Maturation of dendritic cells abrogates C1q production in vivo and in vitro
Blood, May 15, 2004; 103(10): 3813 - 3820.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M. Huber, I. Bahr, J. R. Kratzschmar, A. Becker, E.-C. Muller, P. Donner, H.-D. Pohlenz, M. R. Schneider, and A. Sommer
Comparison of Proteomic and Genomic Analyses of the Human Breast Cancer Cell Line T47D and the Antiestrogen-resistant Derivative T47D-r
Mol. Cell. Proteomics, January 1, 2004; 3(1): 43 - 55.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Puig-Kroger, T. Sanchez-Elsner, N. Ruiz, E. J. Andreu, F. Prosper, U. B. Jensen, J. Gil, P. Erickson, H. Drabkin, Y. Groner, et al.
RUNX/AML and C/EBP factors regulate CD11a integrin expression in myeloid cells through overlapping regulatory elements
Blood, November 1, 2003; 102(9): 3252 - 3261.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Grolleau, D. E. Misek, R. Kuick, S. Hanash, and J. J. Mule
Inducible Expression of Macrophage Receptor Marco by Dendritic Cells Following Phagocytic Uptake of Dead Cells Uncovered by Oligonucleotide Arrays
J. Immunol., September 15, 2003; 171(6): 2879 - 2888.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. L. Wilson and H. C. O'Neill
Identification of differentially expressed genes representing dendritic cell precursors and their progeny
Blood, September 1, 2003; 102(5): 1661 - 1669.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Chaussabel, R. T. Semnani, M. A. McDowell, D. Sacks, A. Sher, and T. B. Nutman
Unique gene expression profiles of human macrophages and dendritic cells to phylogenetically distinct parasites
Blood, July 15, 2003; 102(2): 672 - 681.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. M. Milner and A. J. Day
TSG-6: a multifunctional protein associated with inflammation
J. Cell Sci., May 15, 2003; 116(10): 1863 - 1873.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
D. von Bubnoff, H. Matz, J.-P. Cazenave, D. Hanau, T. Bieber, and H. de la Salle
Kinetics of Gene Induction After Fc{epsilon}RI Ligation of Atopic Monocytes Identified by Suppression Subtractive Hybridization
J. Immunol., December 1, 2002; 169(11): 6170 - 6177.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Fleckenstein, O. Molberg, S.-W. Qiao, D. G. Schmid, F. von der Mulbe, K. Elgstoen, G. Jung, and L. M. Sollid
Gliadin T Cell Epitope Selection by Tissue Transglutaminase in Celiac Disease. ROLE OF ENZYME SPECIFICITY AND pH INFLUENCE ON THE TRANSAMIDATION VERSUS DEAMIDATION REACTIONS
J. Biol. Chem., September 6, 2002; 277(37): 34109 - 34116.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. H. Ahn, Y. Lee, C. Jeon, S.-J. Lee, B.-H. Lee, K. D. Choi, and Y.-S. Bae
Identification of the genes differentially expressed in human dendritic cell subsets by cDNA subtraction and microarray analysis
Blood, August 13, 2002; 100(5): 1742 - 1754.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. W. Sanders, E. C. de Jong, C. E. Baldwin, J. H. N. Schuitemaker, M. L. Kapsenberg, and B. Berkhout
Differential Transmission of Human Immunodeficiency Virus Type 1 by Distinct Subsets of Effector Dendritic Cells
J. Virol., June 27, 2002; 76(15): 7812 - 7821.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
E. A. Roig, E. Richer, F. Canonne-Hergaux, P. Gros, and M. F. M. Cellier
Regulation of NRAMP1 gene expression by 1{alpha},25-dihydroxy-vitamin D3 in HL-60 phagocytes
J. Leukoc. Biol., May 1, 2002; 71(5): 890 - 904.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
F. Le Naour, F. Brichory, D. E. Misek, C. Brechot, S. M. Hanash, and L. Beretta
A Distinct Repertoire of Autoantibodies in Hepatocellular Carcinoma Identified by Proteomic Analysis
Mol. Cell. Proteomics, March 1, 2002; 1(3): 197 - 203.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Ehrlich, K. L. Buchanan, F. Tsien, G. Jiang, B. Sun, W. Uicker, C. M.R. Weemaes, D. Smeets, K. Sperling, B. H. Belohradsky, et al.
DNA methyltransferase 3B mutations linked to the ICF syndrome cause dysregulation of lymphogenesis genes
Hum. Mol. Genet., December 1, 2001; 10(25): 2917 - 2931.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.